BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,185,199	0	T	G	24.3%	240.8 / 19.8	116	T35P (ACC→CCC)	thiM	hydroxyethylthiazole kinase
Reads supporting (aligned to +/- strand): ref base T (15/72); new base G (28/0); total (44/72)
Fisher's exact test for biased strand distribution p-value = 3.33e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 119 total aligned reads displayed.

ATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATC  >  NC_000913/2184950‑2185447

aTAAAAGTTCATGACAAAAAGGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTGCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑tt                                                                                                                                                                                                                                                                     <  2:290831/251‑1 (MQ=255)

  aaaaGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTg                                                                                                                                                                                                                                                                   <  1:604918/251‑1 (MQ=255)

  aaaaGTTCATGACAAAAACGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTg                                                                                                                                                                                                                                                                   >  1:620281/1‑251 (MQ=255)

   aaaGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGc                                                                                                                                                                                                                                                                  <  1:265796/251‑1 (MQ=255)

        tCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTTAACGG‑TTTTcaccc                                                                                                                                                                                                                                                              >  1:583189/1‑250 (MQ=255)

         cATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACcaca                                                                                                                                                                                                                                                            <  1:465489/251‑1 (MQ=255)

                aaaaTGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTgg                                                                                                                                                                                                                                                    <  2:42241/251‑1 (MQ=255)

                aaaaTGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCAca‑tcctttg                                                                                                                                                                                                                                                    >  1:586379/1‑246 (MQ=255)

                  aaTGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTc                                                                                                                                                                                                                                                  <  1:571661/251‑1 (MQ=255)

                      gctgcgATAATCGAGCGCACCCACCGCTACAGGATCAAGCGTCCAGGGTGTTTGAGAGCGTTTTGCGTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGc                                                                                                                                                                                                                                              <  1:135521/248‑1 (MQ=255)

                         gcgATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCGGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGt                                                                                                                                                                                                                                           <  1:171762/251‑1 (MQ=255)

                         gcgATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAGACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGt                                                                                                                                                                                                                                           <  1:342938/251‑1 (MQ=255)

                          cgATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGGGTTTGC‑CCCCC‑TCATTTGTCATGCAgcg                                                                                                                                                                                                                                          >  1:531268/1‑249 (MQ=255)

                           gATAATCGAGCGCGCCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGc                                                                                                                                                                                                                                         <  1:464472/251‑1 (MQ=255)

                             tAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑TCATTGGTCATGCAGTGcac                                                                                                                                                                                                                                       >  1:488282/1‑251 (MQ=255)

                              aaTCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGcac                                                                                                                                                                                                                                       <  2:282630/250‑1 (MQ=255)

                                      gcACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTTCAACACA‑TCATTTGTCATGCAGTGCACAAG‑Aggggg                                                                                                                                                                                                                             >  2:211722/1‑250 (MQ=255)

                                         cccaccGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGTACCACC‑TCCTTGTGCAAGTACTGCACAAA‑AGGGGA‑ATg                                                                                                                                                                                                                         >  2:539158/1‑251 (MQ=255)

                                           caccGCTACTGGATCAAGCGTCCAGGGTGTTGGAGAGCTTTGTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGAGTCAGTGTGCCAGCTTTAATCAACAAGGCACTGTCGATAGCCGCAAACTGACTGGACTCTTCGGTTTCGATAACCATCGCTGTCGATGCACCGAGCGCCAGCAAGGTATTGGATGT‑AAAGT‑TGTTCACCACA‑TCTTTGGTCATGCAGTGCACATG‑AGGGGA‑ATG‑tt                                                                                                                                                                                                                      <  2:217859/251‑1 (MQ=255)

                                              cGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGt                                                                                                                                                                                                                   <  1:192729/251‑1 (MQ=255)

                                               gCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTg                                                                                                                                                                                                                  <  2:450059/251‑1 (MQ=255)

                                                     ggATCAAGAGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCTGCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑aaaa                                                                                                                                                                                                             <  2:387939/249‑1 (MQ=255)

                                                         cAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGgtg                                                                                                                                                                                                        <  1:278775/250‑1 (MQ=255)

                                                                 cAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTg                                                                                                                                                                                               <  1:178185/251‑1 (MQ=255)

                                                                 cAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑TCATTGGTCATGCCGTGCACAAG‑AGGGGG‑ATG‑GTGGTGGAAAAAGG‑GTAAcgcggg                                                                                                                                                                                               >  1:507220/1‑249 (MQ=255)

                                                                     gtgtTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTTCACCCCA‑ACAATGGTCATGCAGGGCCAAAG‑AGGGGG‑AGG‑TTGGGG‑AAAAAGGGGTAAcgcgggggcg                                                                                                                                                                                           >  1:507638/1‑245 (MQ=255)

                                                                           gagaGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCAACCCC‑TCCTTGGTCCTGCAGGGCACAAG‑AGGGGG‑ATG‑TTTGTG‑AAAAAGGGGTAAAGCGGGCGCAGATTgc                                                                                                                                                                                     >  1:512247/1‑251 (MQ=255)

                                                                               gCTTTTTGCTTGCTATACGGCCGCATGCCTCGCCT‑AGTCCCGTGGCTGCGTCAGTGTGCCCACGTTAATCACCAAGGCACTGGCGAGAGCCGCAAACTGACTGTCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGTGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCATATTgcgcgg                                                                                                                                                                                 <  2:324207/251‑3 (MQ=255)

                                                                                cTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAAG‑TTTGCTCCACA‑TCATTGGGCATGCAGAGCACACG‑AGGGGG‑ATG‑TTGGTG‑AAAAAAGGGTAACGCCTGCGCAGATTGCGC‑T‑gt                                                                                                                                                                              >  2:543124/1‑250 (MQ=255)

                                                                                   tttGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAAc                                                                                                                                                                            <  2:66090/250‑1 (MQ=255)

                                                                                     tgcttgctCAACGGCAGCACGCCTCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCCGATTGCGC‑T‑GAAccc                                                                                                                                                                          <  1:88254/250‑1 (MQ=255)

                                                                                      gcttgctCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCcag                                                                                                                                                                        <  1:488938/251‑1 (MQ=255)

                                                                                      gcttgctCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTTCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGG‑ATG‑TTGGGG‑AAAAAGGGGTAACGCGTGCGCAGATTGCGC‑T‑GGACCca                                                                                                                                                                         >  1:585563/1‑250 (MQ=255)

                                                                                       cttgctCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAAG‑GTTGCACCACC‑TCATTTGGCCTGCAGGGCCCAAG‑AGGGGG‑ATG‑TTGGGGGAAAAAGG‑GTAACGCGTGGGCAGATTGCGC‑T‑GGAccccgc                                                                                                                                                                       >  1:207916/1‑248 (MQ=255)

                                                                                       cttgctAAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGGCATGCAATGCACAAG‑AGGGGA‑GTG‑TTGGGG‑AAAAAAGGGTAACACTGGCGCCGATTGCGC‑T‑GAACCcacc                                                                                                                                                                       >  1:82620/1‑249 (MQ=255)

                                                                                        ttgctCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCCCC‑TCATTGGTCATGCAGGGCCAAAG‑AGGGGG‑ATG‑GTGGGG‑AAAAAAGTGTAACGCGGGAGCAGATTGAGC‑T‑GAACCcagc                                                                                                                                                                       >  1:323595/1‑250 (MQ=255)

                                                                                        ttgctCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGG‑AAAGG‑GTTGCACCACA‑ACATTGGGCATGGAGTGCACAAG‑AGGGGG‑GTG‑TTGGGG‑AAAAAGGGGTAACCCGGGGGCAGATTGTGC‑G‑GAAccccgac                                                                                                                                                                      >  2:621213/1‑247 (MQ=255)

                                                                                         tgctCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGGGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCcagca                                                                                                                                                                      <  2:437189/250‑1 (MQ=255)

                                                                                          gctCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCcagcag                                                                                                                                                                     <  1:289267/250‑1 (MQ=255)

                                                                                             cAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGt                                                                                                                                                                   <  2:352430/249‑1 (MQ=255)

                                                                                             cAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGGCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTc                                                                                                                                                                  <  1:471498/250‑1 (MQ=255)

                                                                                                 ggCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑TCATTGGTCCTTCAGTGCACCAG‑AGGGGG‑ATG‑TTGGTG‑AAAAAAGTGTAACGCGGGCGCCGATTTCGC‑T‑GAACCCAGCCGGGCGACtt                                                                                                                                                             >  1:598518/1‑251 (MQ=255)

                                                                                                 ggCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑TCAATGGGCATGCCGTGCACAAG‑AGGGGG‑ATG‑TTGGGG‑AAAAAGGGGTGACGCGTGCGCAGATTGCGC‑T‑GAAACCAGCAAGACGACtt                                                                                                                                                             >  1:629860/1‑251 (MQ=255)

                                                                                                 ggCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAAG‑TTTGCACCACA‑TCCTTGGTCCTGCAGGGCACCAG‑AGGGGG‑ATG‑TTGGTG‑GAAAAGGTGTAACGCGTGGGCAGATTgcggcggaacccgcaaggcgaatt                                                                                                                                                               >  2:59321/1‑230 (MQ=255)

                                                                                                   cagcaCGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGc                                                                                                                                                           <  1:97143/251‑1 (MQ=255)

                                                                                                    agcaCGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTGAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑AGG‑TTGGTG‑AAAAAGGTGTAACGCGGGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCa                                                                                                                                                          <  2:111101/251‑1 (MQ=255)

                                                                                                       aCGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAAGCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCATATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑g                                                                                                                                                     <  2:532787/251‑1 (MQ=255)

                                                                                                           aTCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGG‑AAAGG‑TTTGCACCAAA‑TCCTTTGTCAAGCAGTGCACAAA‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGGGTAACGCGTGCGCAGATTGCGG‑T‑GGACCCAGCAGGGCGGATTGCAT‑A‑Gtttt                                                                                                                                                 >  1:400146/1‑250 (MQ=255)

                                                                                                              gCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑TCATTTGTCATGCCGGGCACCAG‑AGGGGG‑ATG‑TTGGGG‑GAAAAGGTGGAACGCGTGCGCAGATTTCGC‑T‑GAACCCAGCAGGTCGGCTTGCAT‑A‑GTTTtc‑t‑c                                                                                                                                            >  1:301324/1‑251 (MQ=255)

                                                                                                                  gAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTgt                                                                                                                                        <  1:125719/251‑2 (MQ=255)

                                                                                                                  gAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGGCAATCAGGGCACAAA‑AGGGGG‑ATG‑TTGGTG‑AAAAAGGGGTAAAGCGGGCGGAGATTGCGC‑T‑GGACCCAGCAGGGCGACTTGAAT‑A‑GCTTGT‑T‑CCTGc                                                                                                                                        >  1:511905/1‑251 (MQ=255)

                                                                                                                      cgcgTGGCTGCGTCATTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGACTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCAt                                                                                                                                     <  2:411388/251‑1 (MQ=255)

                                                                                                                      cgcgTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑ACATTGGGCATACAGTGGACAAG‑AGGGGG‑ATG‑TTGGTG‑AAAAAGGAGTAAGGCGTGCGGACATTTCGC‑T‑GGACCCCGCAGGGCGACTTGCAT‑A‑GTTTTA‑T‑CCTGcc                                                                                                                                       >  1:312885/1‑249 (MQ=255)

                                                                                                                        ggTGGCTGCGTCAGTGTTCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATaa                                                                                                                                   <  2:584422/250‑1 (MQ=255)

                                                                                                                        cgTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TGTGCACCCCA‑ACATTGGGCAAGCCGTGCACAAG‑GGGGGA‑ATGGTGGGGG‑AAAAAGGGGAAAC‑CGTGCGCAAGGTGCGC‑T‑GAACCCAGCAGGGCGAATTGCAT‑A‑GTTTGT‑T‑CCTGCCATa                                                                                                                                    >  2:320788/1‑250 (MQ=255)

                                                                                                                        cgTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAAG‑GTTGCCCCACC‑TCATTTGGGATTCAGAGCACAAG‑AGGGGG‑ATG‑TTGGGG‑AAAAAGGGGTAAAGCGGGCGCAGCATGCGC‑T‑GAACCCCGCAGGTCGACTTGCCT‑AAGTTT‑C‑T‑CCTGCCATa                                                                                                                                    >  1:484203/1‑250 (MQ=255)

                                                                                                                           ggCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTTCACCACA‑TCATTGGTCATGGAGTGGACAAG‑AGGGGG‑ATGGTGGGGG‑AAAAAGG‑GTAAAGCGGGCGCAGATTGGGC‑C‑GAACCCAGCAGGGCGACTTGCAG‑A‑GGTTGC‑tacttacataac                                                                                                                                   >  1:192574/1‑238 (MQ=255)

                                                                                                                               gCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑Tga                                                                                                                             <  2:31905/249‑1 (MQ=255)

                                                                                                                                 gTCAGGGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCGTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGGGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGAAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑Tgaagaa                                                                                                                         <  1:523278/251‑1 (MQ=255)

                                                                                                                                    aGTGTGCCAACGTTAATCAACAAGGCACTGGCGATGGCCGCAAACTGACTGGCCTCTCCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑GCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGA‑T‑CCTGCCATAACG‑TGAAGAAGCa                                                                                                                      <  2:212680/251‑1 (MQ=255)

                                                                                                                                                  aaTCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TAATTTGGCATGCAGTGCACAAG‑GAGGGA‑ATG‑TTGGGG‑AAAAAGGGGTAAAGCGTGCGCAGATTGCGC‑T‑GAAACCCGCAGGTCGCCTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACC‑TGAAGAAGCAATGACCTGGTGGTc                                                                                                        >  1:276051/1‑251 (MQ=255)

                                                                                                                                                  aaTCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCCCACTC‑TTGGGCATTCAGTGACCAAG‑CGGGGG‑ATT‑TTGGTT‑AAAAAGGGGGCACGCGGGCGCCGCTTTCGC‑T‑GCACCCCCCAGGGCGTCTTTCATTA‑CTTT‑C‑T‑CCTGCCATCACG‑Tgaaagaacactgacctagggggc                                                                                                        >  2:421453/1‑231 (MQ=255)

                                                                                                                                                  aaTCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCAGCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTggtggt                                                                                                         <  2:123346/250‑1 (MQ=255)

                                                                                                                                                   aTCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGGCATGCAGTGCAAAAG‑AGGGGG‑ATG‑GTGGTG‑AAAAAGGGGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTAC‑T‑CCTTCCCTAACA‑TGAAGAAGCAATGACCTGGTGGTcc                                                                                                       >  1:327098/1‑251 (MQ=255)

                                                                                                                                                      aacaaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTg                                                                                                    <  1:115805/251‑1 (MQ=255)

                                                                                                                                                      aacaaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTg                                                                                                    <  1:420239/251‑1 (MQ=255)

                                                                                                                                                        caaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAAG‑GTTGCACCACA‑TCCTTGGTCCTGCAGGGGACAAG‑GGGGGG‑ATG‑TTGGTG‑AAAAAAGTGTAAAGCGTGCGCAGATTGCGC‑T‑GGACCCAGCCGGTCGGCTTGCCT‑A‑ATTTTC‑T‑CCTGCCCTCACG‑TGGAGAAACAAAGCCCtggggggtcggggc                                                                                                  >  2:72254/1‑240 (MQ=255)

                                                                                                                                                              aCTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑TCATTGTGCATGCAGGTCACAAG‑AGGGGGGAGG‑TTGGGG‑AAAAAG‑TGTAACTCGTGCGCAGATTGCGC‑TTGAACC‑AGCAGGGCGACGTTCAA‑A‑GTTTGC‑TTCTTGCAATAACG‑‑GAAGAAAAAAAGACGTGGTGGTCCGTGaatttcct                                                                                            >  2:602031/1‑244 (MQ=255)

                                                                                                                                                                 ggCGATCGCCGCAAACTGACTGGCCGCTTCGGTTTCGATAACCACCGCTGGCGATGCACCGAGCGCCAGCAAGGTATGGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGTCTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGAAATTACCTGGTGGTCCGTGACTTCCCTa                                                                                           <  2:458605/249‑1 (MQ=255)

                                                                                                                                                                 ggCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGTGCGCCATCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGTTCGCCTTGCAT‑A‑GTTTGC‑G‑CCTGCCATAACG‑TGAAGAAGCAATGTCCTGGTTGTCCGTGACTGCCCTAc                                                                                          <  2:496575/250‑1 (MQ=255)

                                                                                                                                                                 ggCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCAGCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTTCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACg                                                                                         <  2:629860/251‑1 (MQ=255)

                                                                                                                                                                   ggATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCt                                                                                       <  2:377199/250‑1 (MQ=255)

                                                                                                                                                                   ggATAGCCGCAAACGGACTGGCCTGTTCGGGTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCt                                                                                       <  2:605192/250‑1 (MQ=255)

                                                                                                                                                                    gATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACC‑ACCTTGGTCATGGAGTGCACAAGGAGGGGA‑‑TG‑TTGGTG‑AAAAAAGAGTAACGCGGGCGCAGATTGGGC‑T‑GAACCCAGCAGGGTGGCTTGCAT‑A‑GTTTGG‑T‑CCTGCCATCACGGTGA‑GAAGCAAAGACCTGGTGGTCCGTGGCTTCCCTACGccg                                                                                      >  1:614384/1‑249 (MQ=255)

                                                                                                                                                                              aaCTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCa                                                                            <  1:369537/251‑1 (MQ=255)

                                                                                                                                                                                 tgactgGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGGG‑AAAAAGGGGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGGGGTCCGTGACTTCCCTACGCTGGCATTATcc                                                                             >  1:388192/1‑247 (MQ=255)

                                                                                                                                                                                 tgactgGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAAG‑ATTGCAACACA‑TCATTTGGCATGCAGGGCACCAG‑AGGGGG‑ATG‑TTGGGG‑AAAAAAGGGTAAAGCGGGCGGAGATTGCGCCT‑GA‑CCCAGCAAGGCGACCTGCCT‑A‑GTTTGC‑T‑CCTGCCCTAAAA‑TGAAGAAGCAATGAACTGGGGGGCCGTGACTTCCCtccgatcgcattttcc                                                                             >  2:447485/1‑232 (MQ=255)

                                                                                                                                                                                   actgGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑TCATTGGGCCTGCAGTGCAAAAG‑AGGGGA‑ATG‑TTGGGG‑AAAAAGGGGTAACGCGTGCGCAGATTGCCC‑T‑GAACCACGCAGGGCGACTTGCAT‑A‑GTTTGC‑T‑CCTTCCATAACG‑TGAAGAAGCAATGACCTGGGGGGCCGTGTATTCCCTACGCTGGCATTATCAAGATc                                                                        >  1:517709/1‑250 (MQ=255)

                                                                                                                                                                                     tgGCCTCTTAGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑GAAAAGGCGTAACGCGTGCGGAGATTGCGC‑T‑GAACCCCGCAGGGCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAAAG‑TGAAGAAGCAATGACCTGGTGGTCCGTGGCTTCCCTACGCTGGCCTTATCCAGATCAgg                                                                     >  1:127165/1‑251 (MQ=255)

                                                                                                                                                                                       gcgttttCGGTTTCGATAACCAGCGTTGGAGATGAACCGAGGGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCTACACA‑TCATTGGTTATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGTTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGTAGGTTGACTTGCAT‑A‑GTTTGC‑T‑CCTGTCATAACG‑TGAAGAAGCAATGTCCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTg                                                                   <  2:439195/246‑1 (MQ=255)

                                                                                                                                                                                       gCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTg                                                                   <  1:223578/251‑1 (MQ=255)

                                                                                                                                                                                             tCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATAc                                                               <  1:306374/249‑1 (MQ=255)

                                                                                                                                                                                                     gATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTACCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTtct                                                     <  2:583189/251‑1 (MQ=255)

                                                                                                                                                                                                      aTAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑CGGGGG‑GTG‑TTGGGG‑GAAAAAGTGTAACGCGTGAGCAGATTTCGC‑T‑GAACCCAACAGGTCGACTTGCAT‑A‑ATTTGC‑T‑CCTGCCATAAAG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACCCCGGCATTATCCAAATCAGGGGGTACgggatcttctt                                                    >  1:607364/1‑243 (MQ=255)

                                                                                                                                                                                                        aaCCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAg                                                  <  1:61063/251‑1 (MQ=255)

                                                                                                                                                                                                           cATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGG‑AAAGG‑GTTGCACCACA‑TCCTTGGTCCTGCAGTGCACAAG‑AGGGGG‑ATG‑TTGGGG‑GAAAAGGGGTAAAGCGTGCGCAGAATGGGC‑T‑GAACCCAGAAGGTCGAATTGCAT‑A‑GATTTC‑T‑CCTGCCATAAAG‑TGCAGAAGCAATGACCTGGTGGACCGTGAATTCCCTACGCTGGCATTATCCAGATCAGGGGGTACGGGGAGTTCTCAGCCt                                               >  1:529482/1‑251 (MQ=255)

                                                                                                                                                                                                            agcGCTTGCGATGCACCGGTCGCCCGCAAGGTATTGGTGGT‑AAAGG‑TTTGCACCACC‑TCATTCGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGAGTGCGC‑T‑GAACCCAGCAGGTCTCCGTGCAT‑A‑GGTTGC‑T‑CCTGCCATAACG‑TGAAGAATCAATGACCTGGTGGTCCGTGACTTCCCTACGCTTGCAGTATCCAGATCCGGTGATACGGGTTTTTCTCAGCCtt                                              <  2:221786/249‑1 (MQ=255)

                                                                                                                                                                                                                  ggCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑TCATTGGTCATGCAGGGCACAAG‑AGGGGG‑ATG‑TTGGGG‑AAAAAGGGGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGGGATACGGGTATTTCTCAGCCTTCCCGc                                         >  1:461405/1‑250 (MQ=255)

                                                                                                                                                                                                                    ggaggCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑GCAGTGGTCATGCAGTGCACAAG‑TGGGGA‑ATG‑TTGGTG‑AAAAAGGTGCAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGTCTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACTAGTATTCCTCAGCCTTCACGCAGa                                      <  2:79957/247‑1 (MQ=255)

                                                                                                                                                                                                                     gATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑GTTGCACCACA‑ACACTGGGCATGCAGTGGCCAAA‑AAGGGA‑ATG‑GTGGGG‑AAAAAGGGGTAACGCGTGCTCAAATTGCGC‑T‑GAACCCACCAGGTCGACTTGCAT‑T‑GGTTGCGT‑CC‑GTCATAACG‑TGAAGAAGCAATGACCTGGTGGTCAGTGACTTCCCTACGCTGGCATTATCAAGAACAGGCGAAACGGGTATTTTTCAGCCTTCACGCAGa                                      >  1:104805/1‑250 (MQ=255)

                                                                                                                                                                                                                      aTGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAg                                    <  1:77170/251‑1 (MQ=255)

                                                                                                                                                                                                                         cACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGc                                 <  1:387577/251‑1 (MQ=255)

                                                                                                                                                                                                                             gAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACC‑CCATTGGTCCTGCAGGGCCCAAG‑AGGGGA‑ATG‑TTGGTGGAAAAAGG‑GTAACGCGGGCGCCGATTGCGC‑T‑GAACCCAGCAGGGCGGCTTTCAT‑A‑GTTTGC‑T‑CCTGCCATAAAG‑GGGAGAAGAAATGACCTGGTGGTCCTGGACGTCCCCACCCTGGCCTTTTCCCGATCAAGGGATACGGGTATTTTTCAGCCTTCCCGCAGAAGGGCAccc                             >  2:224510/1‑251 (MQ=255)

                                                                                                                                                                                                                                 ggcAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑ACATTGGTCATGCAGTGCAAAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAg                         <  2:320989/249‑1 (MQ=255)

                                                                                                                                                                                                                                 gcCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCAGGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAg                         <  1:149716/251‑1 (MQ=255)

                                                                                                                                                                                                                                  ccAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGt                        <  2:531268/251‑1 (MQ=255)

                                                                                                                                                                                                                                  ccAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGt                        <  1:280868/251‑1 (MQ=255)

                                                                                                                                                                                                                                       aaGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGGCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGttt                   <  2:472244/251‑1 (MQ=255)

                                                                                                                                                                                                                                               ggCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCg            >  1:571199/1‑250 (MQ=255)

                                                                                                                                                                                                                                                         gg‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATc  <  1:72254/251‑1 (MQ=255)

ATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAG‑CGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGT‑AAAGG‑TTTGCACCACA‑TCATTGGTCATGCAGTGCACAAG‑AGGGGA‑ATG‑TTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGC‑T‑GAACCCAGCAGGTCGACTTGCAT‑A‑GTTTGC‑T‑CCTGCCATAACG‑TGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATC  >  NC_000913/2184950‑2185447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`