breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 1,142,730 A→C P546P (CCT→CCG rne ← ribonuclease E
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,400,497 T→G H426P (CAC→CCC)  abgT ← p‑aminobenzoyl glutamate:H(+) symporter
MC JC 1,586,443 Δ555 bp [ydeP][ydeQ] [ydeP], [ydeQ]
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,695,565 C→T G169G (GGG→GGA uidA ← beta‑glucuronidase
RA 1,699,275 C→T intergenic (‑95/‑80) malI ← / → malX DNA‑binding transcriptional repressor MalI/PTS enzyme IIBC component MalX
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,823,288 C→T G161D (GGC→GAC)  recA ← DNA recombination/repair protein RecA
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,893,294 Δ1 bp coding (638/1272 nt) ygcN → putative oxidoreductase with FAD/NAD(P)‑binding domain
MC JC 3,090,238 688 bp→74 bp [yggI][endA] [yggI], [endA]
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
JC JC 3,806,046 IS1 (+) +9 bp coding (1014‑1022/1125 nt) waaG ← lipopolysaccharide glucosyltransferase I
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,585,480 G→A Q428* (CAA→TAA)  hsdR ← type I restriction enzyme EcoKI endonuclease subunit
RA 4,602,509 C→T W117* (TGG→TAG)  yjjP ← putative succinate exporter YjjP
RA 4,616,669 G→T L133I (CTA→ATA)  yjjI ← DUF3029 domain‑containing protein YjjI
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 275509 276408 900 26 [0] [2] 3 mmuP S‑methyl‑L‑methionine transporter
* * ÷ NC_000913 276648 277135 488 3 [2] [2] 3 [mmuP]–[mmuM] [mmuP],[mmuM]
* * ÷ NC_000913 277302 278363 1062 3 [2] [2] 3 [mmuM]–[afuC] [mmuM],[afuC]
* * ÷ NC_000913 278612 309899 31288 3 [2] [2] 4 [afuC]–[ecpB] [afuC],insB2,insA2,yagB,yagA,yagE,yagF,yagG,yagH,xynR,argF,argL,ykgS,insB3,insA3,yagJ,yagK,yagL,yagM,ykgV,yagN,intF,yagP,paoD,paoC,paoB,paoA,yagU,ykgJ,ecpE,ecpD,ecpC,[ecpB]
* * ÷ NC_000913 310053 322552 12500 4 [2] [2] 3 ecpA–[ykgF] ecpA,matA,ykgL,ykgO,ykgM,ykgR,insE1,insF1,rclC,rclB,rclA,rclR,ykgE,[ykgF]
* * ÷ NC_000913 322756 372740 49985 3 [2] [2] 3 [ykgF]–[mhpD] [ykgF],ykgG,ykgH,betA,betB,betI,betT,yahV,pdeL,yahB,yahC,yahD,yahE,yahF,yahG,yahH,yahI,yahJ,yahK,yahL,yahM,yahN,yahO,prpR,prpB,prpC,prpD,prpE,codB,codA,cynR,cynT,cynS,cynX,lacA,lacY,lacZ,lacI,mhpR,mhpA,mhpB,mhpC,[mhpD]
* * ÷ NC_000913 1694762 1695469 708 10 [1] [0] 5 uidA beta‑glucuronidase
* * ÷ NC_000913 3423903–3424234 3424415–3424238 5–513 3 [2] [2] 5 rrlD 23S ribosomal RNA
* * ÷ NC_000913 4628621 4629789 1169 29 [0] [1] 3 [ettA] [ettA]
* * ÷ NC_000913 4629841 4634846 5006 3 [2] [1] 29 [ettA]–[rob] [ettA],slt,trpR,yjjX,ytjC,[rob]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2755080 (0.000)26 (0.470) 25/462 0.8 96.3% coding (184/1404 nt) mmuP S‑methyl‑L‑methionine transporter
?NC_000913 372749 = 2 (0.040)coding (635/810 nt) mhpD 2‑hydroxypentadienoate hydratase
* ? NC_000913 1207790 =18 (0.310)19 (0.350) 17/450 1.2 57.8% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 11 (0.210)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780515 (0.260)20 (0.370) 19/450 1.1 61.5% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 11 (0.210)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)19 (0.340) 18/474 1.2 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 46286200 (0.000)28 (0.490) 28/478 0.7 100% intergenic (+73/+235) nadR/ettA DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA
?NC_000913 4634847 = 0 (0.000)coding (464/870 nt) rob DNA‑binding transcriptional dual regulator Rob