| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,699,275 | C→T | intergenic (‑95/‑80) | malI ← / → malX | DNA‑binding transcriptional repressor MalI/PTS enzyme IIBC component MalX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,699,275 | 0 | C | T | 96.8% | 110.2 / ‑6.3 | 31 | intergenic (‑95/‑80) | malI/malX | DNA‑binding transcriptional repressor MalI/PTS enzyme IIBC component MalX |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (13/17); minor base G (1/0); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGCTCTTCAATGGCGGCGTTAACGCGTTCTCCTGTGGCGGTAGAGATTCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGCCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCT > NC_000913/1699036‑1699503 | ccAGCTCTTCAATGGCGGCGTTAACGCGTTCTCCTGTGGCGGTAAAGAGTCGACCTTTGACACTAAACACCAGCGAAAAGGGAATTAAAGCCAGGCCAGCAGACCGGGCAAAATCATGGAAGGTTATTTTTTTGGCGGTAGCCATGCAGAAGACCTACTCCCTGCTTATATGAAAGATAAAACGTTTTACCTTTTATTTATCTTATAACCGCTATTTTCGTGGCGTAATGAGAGTTATTGCTCACTAAAAt > 1:258887/1‑251 (MQ=255) aGCTCTTCAATGGCGGCGTTAACGCGTTCTCCTGTGGCGGTAGAGATTCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAAttt < 1:425880/251‑1 (MQ=255) gTTAACGCGTTCTCCTGTGGCGGTAGAGATTCGCCCTTTGCCACTAAGCCCCAGCGAAACGGGACTTACCGACCCGCCCGCCGCCAGCGCCACATCATGAATGGTTATTTTTTTGGCGGTAGCCCTGCCAATGACCTAACCCCTGGTTTTTTGAAAGATAAAACGTTTTACCTTTTATTTTTTTTTTACCCCCTGTTATCGTTGAGTAATGTGATTTATTTCTCACTCAAATTTTATAAAACGTTTTTtct > 2:122105/1‑251 (MQ=255) gTTAACGCGTTCTCCTGTGGCGGTAGAGATTCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTATTAtct < 1:158349/251‑1 (MQ=255) ggCGGTAGAGATTCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTg < 1:76729/250‑1 (MQ=255) cGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGttt > 1:223726/1‑215 (MQ=255) cGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTGTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGttt < 2:223726/215‑1 (MQ=255) cgttGCCACTAAGCCCCAGCGAAACGGTACTTACCGACACGCCCGCCGCGATCGCAACATCATGAATGTTTATTTTTTTGGCGGTAGCCATGCAGAGGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTGATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTa < 1:85323/249‑1 (MQ=255) cTTTGCCACTAAGCTCCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGACTGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAGTTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTctct > 1:242902/1‑250 (MQ=255) ttGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTAc < 1:350628/250‑1 (MQ=255) gCCACTTAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACg > 1:162849/1‑249 (MQ=255) gAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACg > 2:265021/1‑251 (MQ=255) aaaCGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACgg < 1:265021/251‑1 (MQ=255) ccGCAGCCAGCGCAACATCATGAATGGTTATTGTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCa < 2:3138/251‑1 (MQ=255) cagcGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTG‑TTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTgg > 2:446773/1‑251 (MQ=255) gcgcAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTggg < 1:58807/251‑1 (MQ=255) aCATCCTGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGAAAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGttc > 2:303081/1‑251 (MQ=255) ggTTATGTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCGGATTATGTGATAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAAGTTGATAAAACGTTTTAGCTTCGCTCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGCGTCGTTTTATGACGGCGAAAACAGCTCCGAAAGTCATGCTGTGGGAGTTCTTCCAGCAGtt < 2:2107/251‑1 (MQ=255) tGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGTGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAAcc < 1:303081/250‑1 (MQ=255) aTGCAGATGACCTAATCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGATGCGTAATGTGATTTATGTCCCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGTAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGtacccc > 2:484627/1‑246 (MQ=255) aCTCCCTGATTATGTGACAGATACAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGt > 1:462961/1‑251 (MQ=255) cccTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCg < 1:406813/250‑1 (MQ=255) ggATTATGTGACAGATAAAACGTTTTTCCTTTTATTTCTCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGGTTTATCTTCTCGCGCAAGTTACTGAATCCAGATTGGTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGGTAGGCAAAACCTTCATGTTCCCCGTGGCAGTATTGTCGTTCt < 2:258887/250‑1 (MQ=255) aaaCGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGc > 1:390972/1‑251 (MQ=255) ttttACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGttat > 1:565241/1‑116 (MQ=255) ttttACCTTTTATTTATCTTATACCCGCTATGATCGTTGCGTAATGTGATTTATGTCTCGCTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGttat < 2:565241/118‑3 (MQ=255) ttttATTTAGCTTATACCCTCTATTATCTTTTCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGATGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACTCTGGGGTAGTTCTTTCATGATTTAGGCAAATCCTTCAGGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGATAGTtc < 2:477321/250‑1 (MQ=255) ttatCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTg < 2:171945/217‑1 (MQ=255) tataCCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGcc < 1:446773/249‑1 (MQ=255) ataCCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCatg > 2:185656/1‑251 (MQ=255) tCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCt > 1:196833/1‑251 (MQ=255) | CCAGCTCTTCAATGGCGGCGTTAACGCGTTCTCCTGTGGCGGTAGAGATTCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGCCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCT > NC_000913/1699036‑1699503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |