Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,652,3310TC95.5% 71.3 / ‑5.5 22intergenic (+2346/+397)ydfD/ynfPlysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (11/10);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGAT  >  NC_000913/1652106‑1652566
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           
aCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGaaa                                                                                                                                                                                                                     <  1:120980/250‑1 (MQ=255)
   ggAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGaaaaa                                                                                                                                                                                                                   <  1:99036/249‑1 (MQ=255)
                     cTGTGTGCACTTGGATGGGAGGACATAGAACTGCAACATGGCACAATGATGATCAGGCGCAATTTAGCACAAGACCGTTTCACGGTACAAAAAACACAGGCGGGCACAAAT‑CGGGGAATTCATATTATACAG‑CCCGAAACTC‑CCGCACACCGGAGAAAGATAGCATTAACGAGA‑CAGAGCAAAAAACCAATCCTTAGTGTTC‑ACCTCAGAGAATAAGGCAAAACCGGAAAACAAAAAAGAACATTTGtttt                                                                                                                                                                                               >  2:44111/1‑251 (MQ=255)
                                 ggATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGCGAAATTTAGCAAAAGCCCGTTTCACGGCACCAAAAACACAGGCGGGAACCAAAACGGG‑AATTCATCTTATTAAGGCCA‑CAA‑ACGAAGCTCTCCGGAGGTAGATGAAATTAAAGAGAACGGGGCAA‑GAGGATAAAATTGATGTTC‑AACTCAGAGAGTAAGGCGAAACAAAAAAAACAAAATGCACATTTGTTTTTTAACATGa                                                                                                                                                                                      >  2:95899/1‑248 (MQ=255)
                                   aTGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAgtgt                                                                                                                                                                                 >  1:100501/1‑251 (MQ=255)
                                            cATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGcagaac                                                                                                                                                                                                                          <  1:133995/201‑1 (MQ=255)
                                               aGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTa                                                                                                                                                                      <  1:25172/250‑1 (MQ=255)
                                                              aaCAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTTTTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAAAGCATATCATTGATGTTT‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGGAAAAAATTATTGTGAcc                                                                                                                                                      >  1:462310/1‑251 (MQ=255)
                                                                aaaTGATGATCAGGAGAAATTTAGCAAAAGACCGGTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCAt                                                                                                                                                    <  2:462310/250‑1 (MQ=255)
                                                                                        cAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCCAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCCAAGAGCATATCCTTGCTGTTC‑ACCTCAGAGAGTATGGCAGGACAGAAAAACACAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCCAGAGTAAAAAATTTTTGTGACCCTTTTTCCGTTGACTCAATAAGGccg                                                                                                                            >  1:407602/1‑249 (MQ=255)
                                                                                            aGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAAAGAAA‑ATGAGCAAAAAGCATAACATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGAAACTTTGTTTTTCAACCTGAAGTGTCAGGGAGAGTAAAAAATTATAGTTACCATTTTACCGTTGACTCAATAAGACAGAtgt                                                                                                                        >  2:107622/1‑251 (MQ=255)
                                                                                                cGTTTCACGGTACCAAAAACACAGGCGGGAAACCAT‑AGGGTCATTCATCTTCTTAAA‑CCAGCAA‑TAGAACCCCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAAAGCACATCATTGATGTTCCACT‑CAGAGAGTAGGGCAGAACAGAAAAAAAAAAATTCACCCTTGTTTTTCCAACTGAAGGGGCAGCGAGAGTAAAAAATTATGGTGAACATTTTAACGTTGACTCAATAAAGCAGATGTgggg                                                                                                                    >  1:435586/1‑250 (MQ=255)
                                                                                                                 aaCACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATCAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAATACTAAAATGCGCCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAGTATGGTGACCAGTTTACCGTTGACGCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTg                                                                                                   <  1:404793/251‑1 (MQ=255)
                                                                                                                    acaGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGAc                                                                                                                                        <  1:192345/211‑1 (MQ=255)
                                                                                                                     caGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCgg                                                                                               >  1:333857/1‑251 (MQ=255)
                                                                                                                                       ggTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCAGCGAAAAt                                                                              <  2:333857/251‑1 (MQ=255)
                                                                                                                                            ttcatCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCatat                                                                         >  1:38419/1‑251 (MQ=255)
                                                                                                                                                         g‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGAAGATGAGGGACGCAGAGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATAAGTCGAGACAt                                                             >  2:529939/1‑250 (MQ=255)
                                                                                                                                                         g‑CCAGCAA‑TCGAAGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACAt                                                             >  2:581492/1‑250 (MQ=255)
                                                                                                                                                                            tcCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCg                                           >  1:191953/1‑251 (MQ=255)
                                                                                                                                                                                           cATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCt                            <  2:38419/251‑1 (MQ=255)
                                                                                                                                                                                                                      atCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCATCCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGGCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGAt  <  2:347584/251‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           
ACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAA‑TCGACGCTCTCCGGAGTCAGATGACATTAACGAGA‑CTGAGCAAAGAGCATATCATTGATGTTC‑ACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGAT  >  NC_000913/1652106‑1652566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑