Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,695,565	C→T	G169G (GGG→GGA)	uidA ←	beta‑glucuronidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,695,565	0	C	T	100.0%	64.8 / NA	18	G169G (GGG→GGA)	uidA	beta‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/7);  total (11/7)

ACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACC  >  NC_000913/1695452‑1695802                                                                                                                  |                                                                                                                                                                                                                                              cattcatctcaactggctgACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGc                                                                                                      <  1:144617/233‑1 (MQ=255)  attcatctcaactggctgACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGc                                                                                                      >  1:383019/18‑250 (MQ=255)     catctcaactggctgACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCCGTTCAGTTCGTTGTTCACACAAACGGGGATACGTACACTTTTCCCGGCAAt                                                                                                   >  2:151395/15‑250 (MQ=255)          caactggctgACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACAt                                                                                              <  1:204827/241‑1 (MQ=255)                  tgACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGCAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTg                                                                                      >  1:129890/2‑250 (MQ=255)                                         cgACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGtat                                                                >  1:405272/1‑249 (MQ=255)                                          gACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTACTCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACACACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGAACTCGGCTTCAAATGGCGTATAgc                                                             >  2:490778/1‑251 (MQ=255)                                                     ccacGGTGATATCGTCCACCCAGGTGTTCTGCGTGGTGTAGAGCAGTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGCCTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGc                                                  <  2:129890/251‑1 (MQ=255)                                                                     cACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGGTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGat                                  >  1:168427/1‑251 (MQ=255)                                                                      aCCCAGGAGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCCATAAAATACGGCGTGACATCGGCTTAAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGatt                                 >  2:511470/1‑251 (MQ=255)                                                                          tggTGTTCGGCGTGGTGTAGAGCATTACGCTGCGCTGGATTACGGCATAGTTACAGAAATCATGGACGTAAGACTGCTTTTTCTTGCCGTTTTCGTAGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCCCACAAACGGTGATACGTACCCTTTTCCCGGCAATAACATACGGCGTGACATCGGCCTCAAATGGCGGATCGCCGCCCTGATGTCCCATACCTTCCTGGTTATTg                             >  2:271544/2‑251 (MQ=255)                                                                                  ggCGTGGTGTAGAGCATTACGCGGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGCCTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTGCATCACTTCCTGATTATTGACCCacac                     <  2:343490/251‑1 (MQ=255)                                                                                  ggCGTGGAGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGACATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCCGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCACTAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCacac                     >  1:511604/1‑251 (MQ=255)                                                                                     gtggtgTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACttt                  >  1:517620/1‑251 (MQ=255)                                                                                          gtAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCagttcagttc                                                                                                                                             >  2:479083/1‑123 (MQ=255)                                                                                          gtAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCAGGATAGTCTGCCagttcagttc                                                                                                                                             <  1:479083/123‑1 (MQ=255)                                                                                                    aCGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGa    <  1:151395/250‑1 (MQ=255)                                                                                                     cGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGAcc  <  1:511470/251‑1 (MQ=255)                                                                                                                  |                                                                                                                                                                                                                                              ACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACC  >  NC_000913/1695452‑1695802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑