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breseq version 0.39.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Marginal read alignment evidence (highest frequency 20 of 56 shown, sorted by frequency from high to low) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,027,436 | 0 | T | G | 58.3% | 1.8 / 25.2 | 24 | intergenic (+208/+173) | fre/fadA | NAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase |
| * | NC_000913 | 4,027,448 | 0 | G | A | 52.2% | 1.9 / 30.7 | 23 | intergenic (+220/+161) | fre/fadA | NAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase |
| * | NC_000913 | 4,296,268 | 0 | T | C | 50.0% | ‑5.6 / 36.5 | 26 | intergenic (+474/+168) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| * | NC_000913 | 4,027,449 | 0 | T | G | 47.8% | 5.4 / 27.7 | 24 | intergenic (+221/+160) | fre/fadA | NAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase |
| * | NC_000913 | 4,037,496 | 0 | A | T | 46.9% | 42.6 / 102.7 | 64 | intergenic (+161/‑23) | alaT/rrlA | tRNA‑Ala/23S ribosomal RNA |
| * | NC_000913 | 4,630,340 | 0 | A | C | 43.9% | 59.4 / 21.7 | 59 | G61G (GGT→GGG) | ettA | energy‑dependent translational throttle protein EttA |
| * | NC_000913 | 3,862,664 | 0 | C | G | 41.0% | 28.5 / 12.5 | 40 | A314P (GCA→CCA) | glvC | putative PTS enzyme II component GlvC |
| * | NC_000913 | 4,536,197 | 0 | A | T | 40.0% | 37.9 / 22.1 | 35 | intergenic (‑2144/‑400) | topAI/ythB | toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB |
| * | NC_000913 | 4,465,787 | 0 | G | A | 39.2% | 54.9 / 24.6 | 53 | R132C (CGC→TGC) | treB | trehalose‑specific PTS enzyme IIBC component |
| * | NC_000913 | 786,855 | 0 | A | C | 39.1% | 109.4 / 16.5 | 70 | G247G (GGT→GGG) | gpmA | 2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase |
| * | NC_000913 | 1,130,342 | 0 | A | C | 38.2% | 90.4 / 11.6 | 55 | G174G (GGT→GGG) | flgA | flagellar basal body P‑ring formation protein FlgA |
| * | NC_000913 | 3,177,748 | 0 | C | A | 37.5% | 43.3 / 25.3 | 53 | E55* (GAG→TAG) | nudF | ADP‑sugar pyrophosphatase |
| * | NC_000913 | 3,270,455 | 0 | G | T | 36.8% | 33.9 / 11.7 | 38 | noncoding (138/377 nt) | rnpB | RNase P catalytic RNA component |
| * | NC_000913 | 1,679,304 | 0 | A | C | 36.4% | 57.1 / 10.8 | 44 | Y293S (TAC→TCC) | ydgH | DUF1471 domain‑containing protein YdgH |
| * | NC_000913 | 4,126,210 | 0 | T | G | 35.3% | 100.6 / 12.9 | 68 | T201P (ACC→CCC) | priA | primosomal protein N' |
| * | NC_000913 | 2,889,468 | 0 | T | G | 35.1% | 121.1 / 14.3 | 80 | Y211S (TAC→TCC) | cysI | sulfite reductase, hemoprotein subunit |
| * | NC_000913 | 941,713 | 0 | T | G | 34.9% | 70.6 / 11.7 | 43 | V252G (GTG→GGG) | dmsA | dimethyl sulfoxide reductase subunit A |
| * | NC_000913 | 2,896,475 | 0 | A | C | 34.5% | 82.7 / 11.6 | 55 | G27G (GGT→GGG) | ygcR | putative flavoprotein |
| * | NC_000913 | 1,328,465 | 0 | T | G | 33.9% | 116.2 / 11.5 | 65 | T216T (ACA→ACC) | yciK | putative oxidoreductase |
| * | NC_000913 | 1,435,727 | 0 | G | A | 33.3% | 60.9 / 11.8 | 36 | intergenic (‑108/+33) | uspF/ompN | universal stress protein F/outer membrane porin N |
| Marginal new junction evidence (sorted from low to high skew) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 696741 | 40 (0.670) | 5 (0.120) | 5/338 | 4.1 | 13.3% | intergenic (‑1/+15) | metU/glnW | tRNA‑Met/tRNA‑Gln |
| ? | NC_000913 | 697135 = | 38 (0.930) | intergenic (‑2/+378) | metT/asnB | tRNA‑Met/asparagine synthetase B | |||||