breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 56 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,027,4360TG58.3% 1.8 / 25.2 24intergenic (+208/+173)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
*NC_0009134,027,4480GA52.2% 1.9 / 30.7 23intergenic (+220/+161)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
*NC_0009134,296,2680TC50.0% ‑5.6 / 36.5 26intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009134,027,4490TG47.8% 5.4 / 27.7 24intergenic (+221/+160)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
*NC_0009134,037,4960AT46.9% 42.6 / 102.7 64intergenic (+161/‑23)alaT/rrlAtRNA‑Ala/23S ribosomal RNA
*NC_0009134,630,3400AC43.9% 59.4 / 21.7 59G61G (GGT→GGGettAenergy‑dependent translational throttle protein EttA
*NC_0009133,862,6640CG41.0% 28.5 / 12.5 40A314P (GCA→CCA) glvCputative PTS enzyme II component GlvC
*NC_0009134,536,1970AT40.0% 37.9 / 22.1 35intergenic (‑2144/‑400)topAI/ythBtoxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
*NC_0009134,465,7870GA39.2% 54.9 / 24.6 53R132C (CGC→TGC) treBtrehalose‑specific PTS enzyme IIBC component
*NC_000913786,8550AC39.1% 109.4 / 16.5 70G247G (GGT→GGGgpmA2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase
*NC_0009131,130,3420AC38.2% 90.4 / 11.6 55G174G (GGT→GGGflgAflagellar basal body P‑ring formation protein FlgA
*NC_0009133,177,7480CA37.5% 43.3 / 25.3 53E55* (GAG→TAG) nudFADP‑sugar pyrophosphatase
*NC_0009133,270,4550GT36.8% 33.9 / 11.7 38noncoding (138/377 nt)rnpBRNase P catalytic RNA component
*NC_0009131,679,3040AC36.4% 57.1 / 10.8 44Y293S (TAC→TCC) ydgHDUF1471 domain‑containing protein YdgH
*NC_0009134,126,2100TG35.3% 100.6 / 12.9 68T201P (ACC→CCC) priAprimosomal protein N'
*NC_0009132,889,4680TG35.1% 121.1 / 14.3 80Y211S (TAC→TCC) cysIsulfite reductase, hemoprotein subunit
*NC_000913941,7130TG34.9% 70.6 / 11.7 43V252G (GTG→GGG) dmsAdimethyl sulfoxide reductase subunit A
*NC_0009132,896,4750AC34.5% 82.7 / 11.6 55G27G (GGT→GGGygcRputative flavoprotein
*NC_0009131,328,4650TG33.9% 116.2 / 11.5 65T216T (ACA→ACCyciKputative oxidoreductase
*NC_0009131,435,7270GA33.3% 60.9 / 11.8 36intergenic (‑108/+33)uspF/ompNuniversal stress protein F/outer membrane porin N

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 69674140 (0.670)5 (0.120) 5/338 4.1 13.3% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 38 (0.930)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B