| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,027,448 | 0 | G | A | 52.2% | 1.9 / 30.7 | 23 | intergenic (+220/+161) | fre/fadA | NAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase |
| Reads supporting (aligned to +/- strand): ref base G (8/3); new base A (8/4); total (16/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.57e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGCGTTTGCATTTATCTGAGATATAAAAAAACCC‑GCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CCG‑AACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCAT > NC_000913/4027208‑4027695 | ggtGCGTTTGAATTTAT‑TGAGGAAAAAAAAACCCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTTGTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACatt < 2:413186/249‑3 (MQ=18)gATGCGTTTGCATTTATCTGAGATATAAAAAAACCC‑GCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACatt > 1:188813/1‑249 (MQ=255)gATGCGTTTGCATTTATCTGAGATATAAAAAAACCC‑GCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGGGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACatt > 1:124509/1‑249 (MQ=255) cGTTTGCATTTATCTGAGATATAAAAAAACCC‑GCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCCGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACattggtg > 1:520718/1‑245 (MQ=255) gacATTTATCGGAGATAAAAAAAACCCC‑GCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACattggtgccg < 1:369544/248‑10 (MQ=18) ttATCTGAGATATAAAAAAACCC‑GCCCCTGACAGGCGGGCAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAAGCCGGATAAAGCGTTTACGCCCCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAAGCCTACattggtgccgtatcgg > 2:501476/1‑236 (MQ=21) gaTAAAAAAAAGCCC‑GCCCCGGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACattggtgccggatcggtagaccgg < 2:413954/251‑24 (MQ=17) tataAAAAAACCC‑GCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCCTTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGCCGCTGTCGCGTCTTCTCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACattggtgccggatcggtagaccggat > 1:276092/1‑226 (MQ=17) ccTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAT‑TGGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcgccgc > 1:124278/1‑251 (MQ=14) gACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAT‑TGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATc > 1:42257/1‑251 (MQ=14) aGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAT‑TGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCgg > 2:452009/1‑251 (MQ=11) aCGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAT‑TGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGttaa < 2:130429/251‑4 (MQ=255) ctatgacgtatctggcaaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCATACAAATAGT‑CCG‑AACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGAccc > 1:90508/22‑249 (MQ=18) atgacgtatctggcaaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CCG‑AACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGAccc < 2:452054/228‑1 (MQ=255) gacgtatctggccaaccatgcacgATGCCACGATGTCGCGTCTTATCGTGCCTACACATAGT‑CCG‑AACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGCGGTTAAACCCGCTCAAACACCGTCGCACTACCCTGACCCAGACCg > 2:556612/24‑251 (MQ=18) acgtatctggcaaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CCG‑AACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGa < 2:37216/235‑1 (MQ=255) aaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CCG‑AACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGAACGATACACAtcgtcg > 1:366348/5‑251 (MQ=255) aaccatgCCCGATGCGACGCTGTAGCGTCTTATCGTGCCTACAAATAGA‑CCG‑AACCGTAGGCCGGATAAGGCGTTTCCGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTCGTGCCGGATCGGTAGACAGGATAAGGCGTTCACGCCGCATCCGGCGAGTGGTTAAACCCGCTCAAACAACGTCGCCATACCCTGACCCCGACCGATACACCtcgtcc > 2:290355/5‑250 (MQ=21) gATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CCG‑AACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCaa > 1:315492/1‑250 (MQ=255) cGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CCG‑AACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTg > 1:149309/1‑250 (MQ=255) gACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CCG‑AACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGCCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGa > 2:489059/1‑250 (MQ=255) tGTCGCGTCTTATCGTGCCTACAAATAGT‑CCG‑AACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCt > 1:130810/1‑251 (MQ=255) gtATCGTGCCTACAAATAGT‑CCG‑AACCGTAGGCCGGATAAGGGGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAGCACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGACCGTCTTTGCGTTc < 2:29369/249‑1 (MQ=255) atagTGCCTACCAATAGT‑CCG‑AACCGTAGGCCGGATAAAGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGCCGCTAGCGCGTCTTATCAGGCCTACATTGGCGCCGGACCGGTAGACCGGATACGGCGTTCACGCCGCATCCGGCAACTGGTTAAACTCGCTCAAACACCGTCGCAATACCCTGACCAGGACCGCTCCACATCGTCGCCAGACAAACCTGACCGTCTTTGCGTTcgat > 2:3249/4‑248 (MQ=21) | GATGCGTTTGCATTTATCTGAGATATAAAAAAACCC‑GCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CCG‑AACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCAT > NC_000913/4027208‑4027695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |