Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,630,3400AC43.9% 59.4 / 21.7 59G61G (GGT→GGG) ettAenergy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base A (22/10);  new base C (0/25);  total (22/37)
Fisher's exact test for biased strand distribution p-value = 2.62e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAA‑TCGGCATCCGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCG  >  NC_000913/4630092‑4630590
                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                            
aGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAA‑TCGGCATCCGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCCGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCAcc                                                                                                                                                                                                                                                            >  2:186134/1‑251 (MQ=255)
 gCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAA‑TCGGCATCCGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCt                                                                                                                                                                                                                                                           <  1:375406/251‑1 (MQ=255)
    gTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAA‑TCGGCATCCGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑c                                                                                                                                                                                                                                                        <  1:390239/250‑1 (MQ=255)
                tATGACCGTCGTGAGCCTGAATGATCTCTGCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAA‑TCGGCATCCGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTATTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATTTCTGGCGGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATc                                                                                                                                                                                                                                           <  1:354943/250‑1 (MQ=255)
                tATGACCGTCGTGAGCCTGAATGATCTCGTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAA‑TCGGCATCCGGATCGGCGTACAGCGCATCCACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCGT‑CGATGT‑CTTTATc                                                                                                                                                                                                                                           <  2:259266/250‑1 (MQ=255)
                         cgtGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAA‑TCGGCATCCGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGGTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGc                                                                                                                                                                                                                                  <  2:246320/250‑1 (MQ=255)
                              gCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAA‑TCGGCATCCGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAAt                                                                                                                                                                                                                            >  2:344217/1‑251 (MQ=255)
                                      gcttcctcccAGACGGCTTTGTCCGGCGGCCAGTTTGTAAAAA‑GCGCCTGCCGGAGCGGCGTCCACCGCAT‑CCCTTCCACCCAGGCGTTTCGGGG‑CTTTAACCCCTTCTAAAACC‑TCTTCTTCAA‑TGGCCCCCCGCAGGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCATTTTTG‑ATGTCTGGCGGCGGCCGCG‑CTTC‑CCTTGCGATGT‑CTTTATAAATGCCCGCCATAATGCgcagca                                                                                                                                                                                                                    <  2:55471/243‑1 (MQ=255)
                                             cccAGGCGGCCTTGTTCAGCGGCCATTTTTACAAAA‑CCGGCATCCGGATCGGCTTACAGCGCATCCACTTC‑ATCCAGGCTTTTAAGGG‑CGTTACCCCTTTCTAAACCC‑GCTTCTTCAA‑GGGACTCACGCCGGGTGTGTTCCGGG‑TT‑CAGCGGCGGTTCCGGCGGCAGATAA‑CCATTTTTG‑ATGTGTGGCTGCGGACGCG‑CTTCCCCTT‑GGTTGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGa                                                                                                                                                                                                             <  1:15673/250‑1 (MQ=255)
                                               caggcgcccTTGTTCGGCGCCCAGCTT‑TCAAAAATCGGCATCCGAATCGGCGTACAGCGCATACCCTTC‑ACCCAGGCGTTTCGGGG‑CTTAAACCACTTCTGAAACC‑GCTTCTACAA‑TGGCCTCACGCACGGTGTTTTCCGGG‑TT‑CAGCTGCGTTTCCTGCGGCAGATAA‑CAATTTTTG‑ATGTCTGGCTGCGGACGCG‑CTTCCCCTT‑CGTTTT‑CTTTATCAGTGCCCGCCATAATGCGCAGCAGGGTGGACt                                                                                                                                                                                                           <  1:103901/244‑1 (MQ=255)
                                                            tCAGCGGCCAGCTTGTCAAAA‑TCGCCATCCGGATCGGCGTACAGCGCACACCCTCC‑ACCCAGGCTTTTCAGGG‑CGTTACCCCCTTCTAAAACC‑GCTTCTTCAA‑TGGCCTCACGCCCGGTGTGTTCCGGT‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCCCCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCAt                                                                                                                                                                                               <  1:35701/250‑1 (MQ=255)
                                                                cGGCCAGCTTGTCCAAA‑TCGGCATCCGGATCGGCGTACAGCGCATACCCTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGGGGACTTACCCGCGCCATTc                                                                                                                                                                                             >  2:333366/1‑248 (MQ=255)
                                                                     aGCTTGTCAAAA‑TCGGCATCCGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGAccc                                                                                                                                                                                       >  2:130169/1‑249 (MQ=255)
                                                                     aGCTTGTCAAAA‑CCGGCACCGGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCTCTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCGGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCCCCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAg                                                                                                                                                                                     <  1:250494/251‑1 (MQ=255)
                                                                         tgtaaaaa‑TCGGCACCAGGACCGGCGTAAAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CTTTAACCACTTCTAAAACC‑GCTTCTTCAA‑TGGACCCACGCACGGTGTGTTCCGGG‑TT‑CAGCGGCGTTTCCTGCGGAAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑AGATGT‑TTTTATAAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCGGGac                                                                                                                                                                                  <  1:558476/246‑1 (MQ=255)
                                                                             aaaacccGGCACCCGGATCGGCGAACAGCGCATACCCTTC‑ATCCGGGCGTTTCGGGG‑CGTTACCCCCTTGTGAACCC‑GTTTCTTCAA‑GGGCCTCACGCGGGGTGTGTTCCGGT‑TT‑CAGCGGCGGTTCCTGCGGCAAAAAC‑CCATTCTTG‑ATGTGTGGCGGCGGCCGCT‑CTTCCCCTT‑GGATGT‑TTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCa                                                                                                                                                                              <  2:505175/245‑1 (MQ=255)
                                                                                     gCATCCGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGc                                                                                                                                                                      >  1:548236/1‑251 (MQ=255)
                                                                                       aTCCGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGccc                                                                                                                                                                    >  1:239267/1‑251 (MQ=255)
                                                                                        cccGGAGCGGCGTACAGCCCATCCCCTTC‑ATCCGGGCGTTTCGGGG‑CGTTACCCACTTC‑GAAACCCGTTTCTTCAA‑GGGCCTCACGCACGGTGT‑TTCCGGGGTT‑CAGCTGCGTTTCCTGCGGCAGATAC‑CCATTTTTG‑AGGTCTGGCGGCGGACGCG‑TTTCCCCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGcccc                                                                                                                                                                   <  2:574085/250‑1 (MQ=255)
                                                                                          cGGATCGGCTTCCAGCGCATCCCCTTC‑CTCCGGGCTTTTCAGGG‑CTTTACCCTCTTCTAAACCC‑GTTTCTTCAA‑TGGACTCCCGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCATTCTTG‑ATGTCTGGCGGCGGACGCG‑CTTCCCCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAg                                                                                                                                                                 <  1:35903/251‑1 (MQ=255)
                                                                                          cGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCCGGACACCAATTTTTGcccc                                                                                                                                                                   >  2:376303/1‑249 (MQ=255)
                                                                                            gATCGGCGACCAGCGCAACCACTTC‑ACCAAGGTGTTTCAGGC‑CGTAACCCACTTCTAAACCC‑TCTTCGACAA‑TGGACCCGGGCCGGGTGTGTTCGGGG‑TT‑CAGCTGCGGTTCCTGGGGCAGATAA‑CAAATCTTG‑TGGTCGGGCGGCGGACGCC‑CTCCCCTTT‑CG‑TGTTCTATACCAATCCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAggg                                                                                                                                                               <  2:119962/251‑1 (MQ=255)
                                                                                             aTCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAggg                                                                                                                                                               >  1:506849/1‑250 (MQ=255)
                                                                                                           ccattccccttctcccgggctttCAGGGGCGTTAACCCTTTCAGAACCC‑G‑TTTTTCAAATGGACTCCCGCAGGTTGTGT‑CCGGG‑TTTCGGCTGCGTTCCCGGCGGAAGATAA‑C‑AATTTTGGATGTCGGGTGGGGGACGCG‑CTCCACCTT‑GGTTGT‑CTTTATAAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCagag                                                                                                                                                  <  2:579808/231‑1 (MQ=255)
                                                                                                                  ttC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGtttt                                                                                                                                         >  2:567289/1‑251 (MQ=255)
                                                                                                                    c‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTc                                                                                                                                       >  1:61511/1‑251 (MQ=255)
                                                                                                                      acccAGGCGTTGCAGGG‑CTTTACCCACTTCTGAACCC‑GCTTCTTCAA‑GGGACTCACGCACGGTGTGTTCGGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTTTGGCTGCGGACGCG‑CTTCCCCTT‑CGATTT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCa                                                                                                                                      <  1:479439/249‑1 (MQ=255)
                                                                                                                       cccgggcttttCAGGG‑CTTTTACCCCTTCGGAACCC‑GTTTCTTCAA‑TGGACTCACGCCCGGTGTTTTCGGGG‑TT‑CAGTTGCGGTTCCTGCGGCAGATAA‑CCAATTTTG‑ATGTCGGGCTGCGGCCGCG‑CTTCCCCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCaa                                                                                                                                     <  1:14085/243‑1 (MQ=255)
                                                                                                                        ccAGGCGTTTCAGGG‑CGTTAACCCCTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCaa                                                                                                                                     >  2:290293/1‑250 (MQ=255)
                                                                                                                                         gttacccccTTCTAAAGCC‑GCTTCTTCAA‑GGGCCCCCCGCACGTTGTGTTCCGGG‑TT‑CAGCGGCGGTTCCTGCGGCAGATAC‑CCAATCTTG‑ATGTCTGGCGGCGGACGCG‑TTTCCCCTT‑CGATTT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTcggcg                                                                                                                    <  1:245525/243‑1 (MQ=255)
                                                                                                                                          ttAACCACTTCTTAACCC‑TCTTCTTAAA‑TGGCCCCCCGCAGGGTGGGTTCGGGT‑TT‑GAGCTGCGGTTCCGGCGGCAGAGAA‑CTAATCTTG‑TTGTCTGGCTGCGG‑CCCGTCGTCCCTTT‑CGATTT‑CTTTATAAAGCCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTcggcg                                                                                                                    <  2:493254/250‑1 (MQ=255)
                                                                                                                                          ttAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTcggcgg                                                                                                                   >  2:231024/1‑251 (MQ=255)
                                                                                                                                                aCTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCCGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGACCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGaacaac                                                                                                             >  1:300025/1‑251 (MQ=255)
                                                                                                                                                      gAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGCGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGcc                                                                                                       >  2:345400/1‑251 (MQ=255)
                                                                                                                                                      aaaaCC‑GCTTCTTAAA‑TGGACTCACGCCCGGTGTGTCCCGGG‑TT‑CAGCTGCGGTCCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTCCCCCTT‑CGATGT‑CTTTACCAAGGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGcc                                                                                                       <  1:103337/250‑1 (MQ=255)
                                                                                                                                                         aCC‑GTTTCTTCAA‑GGGACTCCCGCACGGTGTGTTCCGGG‑TT‑CAGCTTCGGTTCCGGCGCCAGATAA‑CCATTCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCCCCTT‑CGATGT‑TTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGac                                                                                                    <  1:123824/251‑1 (MQ=255)
                                                                                                                                                          cccgtttcttcAA‑TGGACTCACGCCCGGTGTGTTCCGGG‑TT‑CAGCGGCGTTTCCTGCGGCAGATAA‑CCAATCTTT‑ATGTCGGGCGGCGGACGCG‑CTTCCCCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGac                                                                                                    <  1:556289/246‑1 (MQ=255)
                                                                                                                                                                 cctaaaatgaccccccgcggggTGTGTCCCGGG‑GC‑CAGCGGCGGTTCCGGCGGAAGAAAA‑CCAATCTTT‑GTGTGGGGCGGGGGACGCG‑CTTCCCCTT‑GGATGT‑TTTTACCAAGGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGAt                                                                                               <  2:575413/230‑1 (MQ=255)
                                                                                                                                                                        tGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGt                                                                                        >  2:460959/1‑250 (MQ=255)
                                                                                                                                                                                  cacgttttgtTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAAATAC‑CCAATCTTG‑ATGTCTGGCGGCGGACGCG‑CTTCACCTT‑CGATTT‑CTTTACAAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAAtt                                                                             <  1:108388/244‑1 (MQ=255)
                                                                                                                                                                                   gcgGTTTGTCCCGGG‑TC‑CAGCGGCGGTTCCTGCGG‑AAATAAACCATTTTTT‑ATGTCTGGCTGCGGCCGCG‑CTTCCCCTT‑CGATTT‑TTTTACCAAGCCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAATTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTg                                                                            <  2:278597/250‑1 (MQ=255)
                                                                                                                                                                                           ggttcgggtttcggCTGCGGTCCCGGGGGCAAATAA‑CCATTTTTG‑TTGTCTGGCGGGGGACGCG‑CTTCCCCTT‑CGGTTT‑TTTTATCACTGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCAc                                                                      <  2:140376/238‑1 (MQ=255)
                                                                                                                                                                                               ggg‑TT‑CAGCGGCGGTTCCTGGGGCAGATAA‑CCATTTTTG‑AGGTCTGGCGGCGGACGCG‑CTTCTCCTT‑CGATGT‑TTTTATAAAGCCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGTTATAAACGAATTGAGCCACGTTGGa                                                                <  2:569758/251‑1 (MQ=255)
                                                                                                                                                                                                        gCTGCGGTCCCTGCGGCAGATAA‑CAAATCTTG‑ATGTCGGGCTGCGGACGCG‑CTTCCCCTT‑CGATGT‑CTTTACCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGcc                                                         <  1:566068/251‑1 (MQ=255)
                                                                                                                                                                                                            ggtttcgtggggCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGCCGCG‑CTTCCCCTT‑TGATGT‑CTTTACCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACGTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTa                                                     <  1:572453/241‑1 (MQ=255)
                                                                                                                                                                                                             ggTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTAt                                                    >  2:167288/1‑251 (MQ=255)
                                                                                                                                                                                                                cccTGCGGCAGATAC‑CCAATCTTG‑GTGTCTGGCTGCGGACGCG‑CTTCCCTTT‑CGATGT‑CTTTAACATTCCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCTACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGtt                                                 <  1:381409/250‑1 (MQ=255)
                                                                                                                                                                                                                cccTGCGGCAGATAA‑CCATTTTTG‑TTGTCTGGCTGGGGACGCT‑TTTCCCCTT‑CGATGT‑TTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGtt                                                 <  1:460959/250‑1 (MQ=255)
                                                                                                                                                                                                                   tGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTAt                                              >  1:543582/1‑251 (MQ=255)
                                                                                                                                                                                                                          aaataaccgtttttG‑ATGTCTGGGTCGGGACCCG‑TTTCCCCTT‑CGATGT‑TTTAATCATGGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGAt                                        <  2:506331/239‑1 (MQ=255)
                                                                                                                                                                                                                                        g‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCaa                           >  2:149944/1‑250 (MQ=255)
                                                                                                                                                                                                                                           tGTCTGGCTGCGGCCGCG‑TTTCCCCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAg                         <  1:231024/250‑1 (MQ=255)
                                                                                                                                                                                                                                                ggCTGCGGACGCG‑CTTCACCTT‑CGGTGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAg                   <  1:70776/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  cTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAgtg                 >  2:150774/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   tGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAgtgt                >  1:505580/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      ggACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCACGCCATAATGCGCAGCAGGGCGGACTTTCCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGCAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTTAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGGTTTCAACGGCGAAGTGTatg             >  2:98982/1‑249 (MQ=255)
                                                                                                                                                                                                                                                        aCGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCt           >  1:107047/1‑251 (MQ=255)
                                                                                                                                                                                                                                                         cgcg‑CTTCACATT‑AGAGGT‑TTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTGTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTGCGCCTCTATGTTGATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCtt          <  1:50893/251‑1 (MQ=255)
                                                                                                                                                                                                                                                            g‑cTTCCCCTT‑CGATTT‑CTTTATCAAGGTCCTCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCGCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCttttt       <  1:488055/251‑1 (MQ=255)
                                                                                                                                                                                                                                                                  aCCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAACTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCg  >  2:301947/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                            
AGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAA‑TCGGCATCCGGATCGGCGTACAGCGCATACACTTC‑ATCCAGGCGTTTCAGGG‑CGTTAACCACTTCTGAAACC‑GCTTCTTCAA‑TGGACTCACGCACGGTGTGTTCCGGG‑TT‑CAGCTGCGGTTCCTGCGGCAGATAA‑CCAATCTTG‑ATGTCTGGCTGCGGACGCG‑CTTCACCTT‑CGATGT‑CTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCG  >  NC_000913/4630092‑4630590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑