breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 108 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,699,9220C.79.1% 221.3 / 80.1 92intergenic (+29/+58)bcsG/ldrDcellulose phosphoethanolamine transferase/small toxic polypeptide LdrD
*NC_0009133,877,7560GA78.2% 132.8 / 31.7 80R788R (CGC→CGTgyrBDNA gyrase subunit B
*NC_0009134,472,5080AG78.0% 69.8 / 12.2 41V9A (GTC→GCC) pyrLpyrBI operon leader peptide
*NC_0009131,013,0580GT77.8% 73.1 / 19.3 45R353L (CGC→CTC) pqiAintermembrane transport protein PqiA
*NC_0009132,158,8140GA77.5% 125.1 / 26.2 76T143T (ACG→ACAmdtCmultidrug efflux pump RND permease subunit MdtC
*NC_000913971,1280GT75.3% 111.7 / 36.3 73V93L (GTG→TTG) kdsB3‑deoxy‑manno‑octulosonate cytidylyltransferase
*NC_0009134,234,2130CT74.4% 60.4 / 16.7 39N152N (AAC→AATpgiglucose‑6‑phosphate isomerase
*NC_0009134,060,2800T.74.1% 108.8 / 70.0 58intergenic (+50/‑167)bipA/yihL50S ribosomal subunit assembly factor BipA/putative DNA‑binding transcriptional regulator YihL
*NC_00091321,5200CA73.1% 117.5 / 37.9 78G38G (GGC→GGAribFbifunctional riboflavin kinase/FMN adenylyltransferase
*NC_0009131,514,8970GA72.5% 63.9 / 28.7 52G46S (GGC→AGC) ydcVputative ABC transporter membrane subunit YdcV
*NC_000913595,1650CT70.9% 62.1 / 36.3 55L93L (TTG→TTAcusRDNA‑binding transcriptional activator CusR
*NC_0009131,058,3690GA70.6% 61.3 / 32.2 56A96T (GCG→ACG) torCcytochrome c menaquinol dehydrogenase TorC
*NC_0009132,613,0530CT70.3% 44.9 / 17.4 38A385V (GCC→GTC) hyfRDNA‑binding transcriptional activator HyfR
*NC_000913485,8030CT69.8% 42.9 / 29.5 44H15Y (CAC→TAC) acrRDNA‑binding transcriptional repressor AcrR
*NC_0009131,569,9420G.69.0% 39.0 / 34.0 29coding (548/1536 nt)gadCL‑glutamate:4‑aminobutyrate antiporter
*NC_0009132,605,3910CT69.0% 56.6 / 35.0 60A194V (GCG→GTG) hyfDhydrogenase 4 component D
*NC_0009133,628,6070TC69.0% 102.2 / 56.1 88N311S (AAC→AGC) rbbAribosome‑associated ATPase
*NC_0009132,967,6380GT68.8% 86.8 / 61.5 81S266* (TCG→TAG) ptsPphosphoenolpyruvate‑protein phosphotransferase PtsP
*NC_0009134,218,4840GA68.4% 119.7 / 86.6 115intergenic (+71/‑112)aceA/aceKisocitrate lyase/isocitrate dehydrogenase kinase/phosphatase
*NC_0009131,601,8320CA68.0% 45.0 / 23.2 26coding (343/1536 nt)lsrAAutoinducer‑2 ABC transporter ATP binding subunit

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4385282 =74 (1.190)4 (0.060) 4/490 3.5 5.1% coding (1444/1503 nt) yjeM putative transporter YjeM
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 426961642 (0.670)4 (0.060) 4/490 3.5 8.6% coding (203/714 nt) aphA acid phosphatase/phosphotransferase
?NC_000913 4269628 = 43 (0.690)coding (215/714 nt) aphA acid phosphatase/phosphotransferase
* ? NC_000913 2996361 =NA (NA)4 (0.060) 4/490 3.5 4.4% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 3485924 87 (1.390)intergenic (‑106/‑196) yhfA/crp OsmC family protein YhfA/DNA‑binding transcriptional dual regulator CRP
* ? NC_000913 = 1469240NA (NA)3 (0.050) 3/488 3.8 10.0% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 1588242 27 (0.430)coding (55/504 nt) ydeR putative fimbrial protein YdeR