Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,234,2130CT74.4% 60.4 / 16.7 39N152N (AAC→AAT) pgiglucose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base C (4/6);  new base T (5/24);  total (9/30)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGA  >  NC_000913/4233968‑4234455
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
aaaGAGTGAGATCTGGCGGGAGCGATTACGTCGATGTTCTCTGGCGCGAAGATCAACCGCACTGAAAACCGAGCCGAGCTGCACGTAGCGCTGCGTAACAGTAGAAATCCCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAAAGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGATATACCGGCAAAGCAATCACTGACGTATTGAACATCgg                                                                                                                                                                                                                                                   >  2:66348/1‑251 (MQ=255)
  agagTGAGATCTGGCGGGCGCGATTAAGTCGATGTTATCTGGCGAGAAGATCAACCGCACTGAAAACCGCGACGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCgggg                                                                                                                                                                                                                                                 >  1:30176/1‑250 (MQ=255)
       gCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATcggc                                                                                                                                                                                                                                            >  1:93761/1‑251 (MQ=255)
              ggcgggcgCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCt                                                                                                                                                                                                                                      <  2:78771/250‑1 (MQ=255)
                cgggcgCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGGGAATATCGGGATCGGCGGGTCttac                                                                                                                                                                                                                                   >  2:342571/1‑248 (MQ=255)
                   gcgcGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATcggcgggttttgcctc                                                                                                                                                                                                                                >  1:119224/1‑241 (MQ=255)
                           aaGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTTACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCAt                                                                                                                                                                                                                        <  1:557521/250‑1 (MQ=255)
                                   gTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTTTGAccacagccccaaaatgggc                                                                                                                                                                                                                 >  1:19342/1‑233 (MQ=255)
                                     tctcTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCACTACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTGCTgtcatccgcccccacactgggacc                                                                                                                                                                                                              >  2:318572/1‑228 (MQ=255)
                                     tctcTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGGTGATGGCAAAGACGTAATGCCGGAAGGCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGAc                                                                                                                                                                                                              <  2:200121/250‑1 (MQ=255)
                                        ctGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTTATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAATATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTTAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGAcc                                                                                                                                                                                                             <  1:322642/248‑1 (MQ=255)
                                           gCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGct                                                                                                                                                                                                       <  2:435969/251‑1 (MQ=255)
                                             gagaAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGctct                                                                                                                                                                                                     <  2:521373/251‑1 (MQ=255)
                                                                     cgcgCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGaa                                                                                                                                                                            <  2:77158/251‑1 (MQ=255)
                                                                           gTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCa                                                                                                                                                                     <  2:184261/251‑1 (MQ=255)
                                                                                 cACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGt                                                                                                                                                               <  1:79187/251‑1 (MQ=255)
                                                                                            gCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTc                                                                                                                                                     <  2:493285/250‑1 (MQ=255)
                                                                                                                     ttGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGc                                                                                                                            <  2:469229/250‑1 (MQ=255)
                                                                                                                     ttGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGc                                                                                                                            <  1:359950/250‑1 (MQ=255)
                                                                                                                      tGGTTGATGGCAAAGACGTAAGGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCt                                                                                                                           <  1:318572/250‑1 (MQ=255)
                                                                                                                       gggtGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTGCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATAGCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCGGCGTCCGTAC‑AAATACCACCTGACC‑ATGCTCTTTGTGTCTAACGGCGATGGGACTTACATCGCGGaatggctga                                                                                                                         <  2:604688/248‑8 (MQ=255)
                                                                                                                                    gACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTAAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGTCCTCCGGCCCCAAC‑TGGGGAACGGACCGCTGCGTGCGCAC‑AAAAAACACCACAACCATG‑ACTTTTTTTCTTACATCACTGGGACTCACATCCCCGAAGTGCCGCAAAAAGaaaaaa                                                                                                             >  2:409787/1‑248 (MQ=255)
                                                                                                                                         aaTGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTTCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGTCTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGaa‑a                                                                                                      <  2:323922/251‑1 (MQ=255)
                                                                                                                                         aaTGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGAGGGGACTCACATCGCGGAATTGCTGAAAAAAGTAAACCCGGaa                                                                                                        <  2:93761/250‑1 (MQ=255)
                                                                                                                                          aTGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCGAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑Ac                                                                                                     <  1:368345/251‑1 (MQ=255)
                                                                                                                                             ccGGAAGTCAACGCGGTGATGGAGAAGATGAAACCCTTCTCAGAAGCGTTTATTTCCGGTGAGTGGAAAGGTTATTCCGGCAAAGCAATCACTGTCGCAGTGAATATTGTGATCGGCGGTTCTGACCGC‑GGCCCATACATGGTGACCGATGGTCTTCGGCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTGTCTGACGTCGATGGGACTCACAGCGCGGAAGTGATGAATAAAGTaggcacggattccac                                                                                                   <  2:610606/251‑15 (MQ=255)
                                                                                                                                             ccGGAAGGCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGTCGTGTCTGACGTC‑GGCCCATACATGGTGACCGAAGCTCTTCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGCGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCAc                                                                                                  <  1:356278/251‑1 (MQ=255)
                                                                                                                                                gAAGTCAACGCGGTGCTGGATAATATGAAAACCTGCTCTGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAACCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGCCCGC‑GGCCCATACATGGTGACCGAAGCGCTGCGTCCGTAC‑AAAAACCACCTTAAC‑ATGCACTTGGTTTCTAACGTCGATGTGACTCACAACGCGGAAGTGCCGAAAAAAGTAAACCCGTAA‑ACCActct                                                                                               <  2:585982/251‑4 (MQ=255)
                                                                                                                                                     cAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCACGCTGTTCt                                                                                          <  1:344461/251‑1 (MQ=255)
                                                                                                                                                                 ggAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCACGCTGTTCTTGGTAGCATc                                                                                <  1:524328/249‑1 (MQ=255)
                                                                                                                                                                                                attTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGTGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGTGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAATCTATGACCa                                                <  1:411528/250‑1 (MQ=255)
                                                                                                                                                                                                    ccGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGAGGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGc                                            <  2:555511/250‑1 (MQ=255)
                                                                                                                                                                                                               aaaGGTTATACCGGCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGGTCTTAAACC‑GGCCCATACATGGGCAACCAAGCGCTGCGTGCCAACGAAAAACC‑CCCGAAC‑AAAAAATTTATTTCTTACGATGAGGGGACACACATCGCGGAAGTGCTGAAAAAAAGAAACCCGGAACAAAAC‑CTGTTCTTGGTAGCATCTAAAACATTCACCAATCAAGAAAATAAGACCCACACCCAAAAcgcgcg                                >  1:615017/1‑251 (MQ=255)
                                                                                                                                                                                                                            gCAAAGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTg                   <  1:7195/251‑1 (MQ=255)
                                                                                                                                                                                                                               aaGCAATCACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCTCATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGaaa                <  2:344953/251‑1 (MQ=255)
                                                                                                                                                                                                                               aaGCAATCACTGACGTAGGGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGaaa                <  1:72587/251‑1 (MQ=255)
                                                                                                                                                                                                                                  caagcACTGACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTGGTTTCGAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCACGCTGTTCTTGGTAGCATCTAAAACCGTCACCACTCAGGGAACTATGTCCAACGCCCATAGCGCGCTTGACTGGTTCCTGAAAGCg             <  2:573955/247‑1 (MQ=255)
                                                                                                                                                                                                                                          gACGTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGtg      <  1:479706/250‑1 (MQ=255)
                                                                                                                                                                                                                                             gTAGTGAATATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGtgatga  <  2:122701/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
AAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTC‑GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAC‑AAAAACCACCTGAAC‑ATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAA‑ACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGA  >  NC_000913/4233968‑4234455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑