Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,601,8320CA68.0% 45.0 / 23.2 26coding (343/1536 nt)lsrAAutoinducer‑2 ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base A (7/10);  minor base . (4/4);  total (12/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGGGGGAGGTCCACGCCCTGCTCGGCGGCAATGGTGCCGGTAAATCGACGTTAATGAAGATTATTGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCTGGGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTA  >  NC_000913/1601596‑1602066
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          
aGGGGGAGGTCCACGCCCTGCTCGGCGGCAATGGTGCCGGTAAATCGACGTTAATGAAGATTATTGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCTGGGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAAAAGCTCTCCATGc                                                                                                                                                                                                                                >  1:156501/1‑249 (MQ=255)
                                ggTGCCGGTAAATCGACGTTAATGAAGATTATTGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCTGGGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAAAAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCt                                                                                                                                                                                               <  1:136784/250‑1 (MQ=255)
                                    ccGGTAAATCGACGTTAATGAAGATTATTGCCGGAATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCTGGGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTTCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAAAAGCTCTCCATGCAGAAAATGAAGTACTTGCTGGCGGCGCTGGGCTGcc                                                                                                                                                                                            >  1:467863/1‑249 (MQ=255)
                                                cGTTAATGAAGAATATTGCCGGTATTACCCCTGCTGAGAGCGGTACGCCGGAGATTGAGGGTATCAACTACGTAAGATTAACGCCAGTTCATGAGCATCAGATGCGTATTTAGCTCGTGCCCAAGGTTCCTCTGCGTTTCCCAAGCCTGTCGATAAAAGAAAACATACTGTTTGTGCTGGCAAAAAAAAAGCGCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCatctttatctgagt                                                                                                                                                                              <  2:459763/251‑14 (MQ=255)
                                                               gtGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCTGGGTTTTTAGCTCGTTCCCCATGTACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAATCATCCTGTTTGGGCTGGCAAAAAAAAAGCTCTCCATGCATAAATTGAAGAACTTGCTGGCGGTGCTTGGCTGCCAGTTTGATCGGCATATTCTCGCAGGATc                                                                                                                                                                <  2:548513/249‑1 (MQ=255)
                                                                    ggTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCTGGGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAA‑CAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGAt                                                                                                                                                         >  1:38861/1‑251 (MQ=255)
                                                                    ggTATTACCACTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCTGGGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAA‑AGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGAt                                                                                                                                                         <  1:312856/251‑1 (MQ=255)
                                                                                 ctgATGGCGGTACGCAGCACATTGCGGGCAACCACTACGTCAGATTAAGGCCCGTTCATGCTCATCAGCTGGGTATTTATCTCGTTCCACAGGAACCGCTGATTTTCCCAAGCCTGTCGATAAACGAACACATCCTGTTTGGGCTGGCCCAAAAAAAGCTCTCCATGCAGAAAATGCAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCGGCATAGCCTGGAAGAATCGCTGGAGGTCGACGGTCGc                                                                                                                                             >  2:622441/1‑251 (MQ=255)
                                                                                                                     ccggcAGATTAACGCCATTTCATGCTCATCAGCGGGGTATTTATCTCGTTCCCCAGGAACCGCTGCTGTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAAAAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCTCGGGCTGATGCGCGAc                                                                                                         <  2:325059/247‑1 (MQ=255)
                                                                                                                             ttAACGACAGTTCATGCTCATCAGCTGGGAATTTATCTCGTTCCCAAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAAAAGCTCTCCATGAAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGAGCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGAt                                                                                                 >  2:253711/1‑251 (MQ=255)
                                                                                                                                         cATGCTCATCAGCTGGGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAAAAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGa                                                                                     <  2:430948/251‑1 (MQ=255)
                                                                                                                                            gCTCATCAGCTGGGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAAAAGCTCTCCATGCAGAAAAGGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGa                                                                                  <  2:324696/251‑1 (MQ=255)
                                                                                                                                                   aGCTGGGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAAAAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAcc                                                                           <  1:76894/251‑1 (MQ=255)
                                                                                                                                                                   cGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAA‑AGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGc                                                          >  1:469979/1‑251 (MQ=255)
                                                                                                                                                                               aCCGCTGCTTTTCCCAAGCTTGTCGATAAAAAAAAACAGCCTGTTTTGGCTGGCAAAAAAAAAGCGCGCCACGCAGAAAATGAATAACCTGTTGGCGGGGCTGGGCTGCCAGTTTGATCTGCATAGTTTGGCAGTCTCGCTGTATGGCGCCGAGCGCCAAATGGTTTAAATCTCCCGCGGGCTGATGCGCGCCTCGCGGCTTCTGTGCCCCGATGAACTTACCGCCTCGCTTACCCCTGCGGAAACCGAAc                                               <  1:622441/251‑1 (MQ=255)
                                                                                                                                                                                  gctgctTTTCCCCAGCCTGTCGCTAAAAGACAACATCCTGTTTGGGCTGGCAAAAAAAAAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCt                                            >  1:499810/1‑251 (MQ=255)
                                                                                                                                                                                                    gTCGATCAAAGAAAACATCATGATTGGGATGGCAAAAAAAAAGCTCTCCATGCAGACACTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTACATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGCAATCCTCCGCGGGCTGCTGCGCGAATCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCa                            >  2:391910/1‑249 (MQ=255)
                                                                                                                                                                                                        aTAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAA‑ATCTCTCCATGCAGAAAATGAAGAACTTGCTGGAGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGCGGAAATCCTCCGCGGGCTTATGCGCTACTCGCGTATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACACTTGTTTAGTCGCTTGCAAGAgctg                     >  1:1656/1‑251 (MQ=255)
                                                                                                                                                                                                        aTAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAA‑AGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCCTAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCAAGAgctg                     >  1:323139/1‑251 (MQ=255)
                                                                                                                                                                                                          aaaaGAAAACATCCTGTTTGGGCTGGCAAAAAAAAAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCAAGAgctgc                    <  1:314181/251‑1 (MQ=255)
                                                                                                                                                                                                           aaaGAAAACATCCTGTTTGGGCTGGCAAAAAAA‑AGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATc                                                                                          <  1:56191/179‑1 (MQ=255)
                                                                                                                                                                                                           aaaGAAAACATCCTGTTTGGGCTGGCAAAAAAA‑AGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATc                                                                                          >  2:56191/1‑179 (MQ=255)
                                                                                                                                                                                                                   cATCCTGTTTGGGCTGGCAAAAAAAAAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCCAGAGCTGCTTGCTACTg           >  1:335416/1‑251 (MQ=255)
                                                                                                                                                                                                                        ggttggggCTGGCAAAAAAA‑AGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCGGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGAGCCTCGATGAACCTACCGCCGCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGGCGCTTGCAAGAGCTGCTTGCTACTGGCGTgg     <  2:570113/246‑1 (MQ=255)
                                                                                                                                                                                                                         gTTTGGGCTGGCAAAAAAA‑ACCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTggg    <  2:88013/251‑1 (MQ=255)
                                                                                                                                                                                                                             gggCTGGCCAAAAAAAAGCTCTCCATGCAGAAAATGAAGAACTGGCTGGCGGCGCTGGGGTGCCAGTTTGAGCTGCATATGCTGGCAGTAGCGCCGGATGTCGCGGATCGCCAAATGTGGGAAACCCTCCGCGGGCTGATGCGCGACTCGCGGATTCGGATCCGCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTa  <  2:467863/250‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          
AGGGGGAGGTCCACGCCCTGCTCGGCGGCAATGGTGCCGGTAAATCGACGTTAATGAAGATTATTGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCTGGGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTA  >  NC_000913/1601596‑1602066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑