Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,013,0580GT77.8% 73.1 / 19.3 45R353L (CGC→CTC) pqiAintermembrane transport protein PqiA
Reads supporting (aligned to +/- strand):  ref base G (5/5);  new base T (13/22);  total (18/27)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAG  >  NC_000913/1012816‑1013300
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  
tttGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTGATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCg                                                                                                                                                                                                                                             <  1:72467/250‑1 (MQ=255)
               gACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCAGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTc                                                                                                                                                                                                                              <  1:448381/250‑1 (MQ=255)
                    aTTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGAATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATc                                                                                                                                                                                                                        >  2:526856/1‑251 (MQ=255)
                    aTTTATTAGGCTCGAAGAAGCCGTCGACGCTTCTCGCTGGGGTCATTCTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAACGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCGAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGCAGTTGTTGAGTTTGTAGGCCTATGGTCGATGATTGACGTTTTCGTTAt                                                                                                                                                                                                                         >  2:425754/1‑250 (MQ=255)
                        attaGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCgg                                                                                                                                                                                                                    <  1:256346/251‑1 (MQ=255)
                          taGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTg                                                                                                                                                                                                                  >  1:284724/1‑251 (MQ=255)
                                cGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCg                                                                                                                                                                                                            <  1:126995/251‑1 (MQ=255)
                                       gCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTgg                                                                                                                                                                                                     <  2:472533/251‑1 (MQ=255)
                                        ccGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGt                                                                                                                                                                                                    >  1:246008/1‑251 (MQ=255)
                                            cgacgaTTCTCGCTGGGGTCATTCTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGt                                                                                                                                                                                                <  1:297467/251‑1 (MQ=255)
                                                         tGGGGTCATTCTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTa                                                                                                                                                                                    >  1:428240/1‑250 (MQ=255)
                                                             gtctgtCTGTTATGGAGCGAAGGCTCTTATCCGGTCGCTGCGGTGATCTTTCTGGCAAGTATTATGGTGCCACCGTTAAGGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCGTTTGATTTATGAAGTTGTTGAGTTTGTAGGCTTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTATCGGCGCTGGTGCGTATGGGATGTTTAATGat                                                                                                                                                                               <  2:342276/246‑2 (MQ=255)
                                                                   cTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATAGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTAtttt                                                                                                                                                                         >  2:72772/1‑250 (MQ=255)
                                                                        aTGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCg                                                                                                                                                                     <  1:135285/250‑1 (MQ=255)
                                                                               gAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATggg                                                                                                                                                             <  1:100023/251‑1 (MQ=255)
                                                                                  ggCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGc                                                                                                                                                          >  1:268736/1‑251 (MQ=255)
                                                                                  ggCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGCTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGc                                                                                                                                                          >  2:211686/1‑251 (MQ=255)
                                                                                                  atGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCttt                                                                                                                                           >  2:21181/2‑250 (MQ=255)
                                                                                                     cGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAgtc                                                                                                                                       >  2:256515/1‑251 (MQ=255)
                                                                                                     cGGTGAACTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAgtc                                                                                                                                       <  1:351318/251‑1 (MQ=255)
                                                                                                              ttCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATGTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATa                                                                                                                                 <  2:284724/248‑1 (MQ=255)
                                                                                                              ttCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAAt                                                                                                                               <  1:280597/250‑1 (MQ=255)
                                                                                                                  ggCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACa                                                                                                                           <  1:325906/250‑1 (MQ=255)
                                                                                                                  ggCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCAGTAATGTTTGCTTTAGTCGTCATAATGAc                                                                                                                            <  2:285514/249‑1 (MQ=255)
                                                                                                                         attatGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTc                                                                                                                   <  1:393760/251‑1 (MQ=255)
                                                                                                                         attatGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGCTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTc                                                                                                                   >  1:593588/1‑251 (MQ=255)
                                                                                                                                    aaCGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCGTTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGt                                                                                                        >  1:17706/1‑251 (MQ=255)
                                                                                                                                                 atCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACCGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTCTGGGAGGTTTACTGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGttt                                                                                           >  1:89816/1‑251 (MQ=255)
                                                                                                                                                  tCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTg                                                                                          >  1:47324/1‑251 (MQ=255)
                                                                                                                                                               gCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGt                                                                              >  1:382565/1‑250 (MQ=255)
                                                                                                                                                               gCTGTATTGGGATGCCAAAGGGCATGGCACGCGCGACAGTGAAAGCATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGCCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTTGGCTCGTc                                                                             >  2:254771/1‑251 (MQ=255)
                                                                                                                                                                      tGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTg                                                                       >  1:250306/1‑250 (MQ=255)
                                                                                                                                                                            gCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCaga                                                                <  1:205712/251‑1 (MQ=255)
                                                                                                                                                                              cAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCAGAGc                                                              <  1:177759/251‑1 (MQ=255)
                                                                                                                                                                                                  aCAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAAGCAGAGCATGAGGAGTTCTGACAGTAt                                          <  2:428240/251‑1 (MQ=255)
                                                                                                                                                                                                       gAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTATGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCGTTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGaa                                      <  2:17706/250‑1 (MQ=255)
                                                                                                                                                                                                       gAAAGAATGCATTTGATTTATGAAGTTGGTGAGTGTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCAGAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGACAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGaa                                      <  2:425286/250‑1 (MQ=255)
                                                                                                                                                                                                                            gAAGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCaaa                 <  2:246008/250‑1 (MQ=255)
                                                                                                                                                                                                                             aaGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCTGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAAt               <  1:425754/251‑1 (MQ=255)
                                                                                                                                                                                                                              aGTTGTTGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATc              <  1:414455/251‑1 (MQ=255)
                                                                                                                                                                                                                               gttgttGAGTTTGTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTAGTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAAGCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGTGGAAGCCAAAATcc             <  2:551756/251‑1 (MQ=255)
                                                                                                                                                                                                                                  gttgAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATcc             >  1:588915/1‑248 (MQ=255)
                                                                                                                                                                                                                                  gttgAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTGTTCGGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAg           <  1:74894/250‑1 (MQ=255)
                                                                                                                                                                                                                                  gttgAGTTTGTAGGACTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCTTCAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGa          <  1:526856/251‑1 (MQ=255)
                                                                                                                                                                                                                                           gTAGGCCTCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTgaag  <  1:520301/250‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  
TTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGAAGGCTCTTATCCCGTCGCTGCGGTGATCTTTCTGGCCAGTATTATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTGACCCGCGTTTGTCGTGGGATCGTCAACCTGAATCAGAGCATGAGGAGTCCTGACAGTATGGAATCTAATAATGGGGAAGCCAAAATCCAGAAAGTGAAG  >  NC_000913/1012816‑1013300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑