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4.4.3 von Kossa staining of sections

Yoshiyuki Nemoto
Tokyo Institute of Technology

4.4.3.1 General information

Calcification tissues such as bone, teeth and scale can be detected by von kossa staining procedure.

4.4.3.2 Reagents and equipments

  • 5% AgNO3
  • Sodium Carbonate-formaldehyde (5% Na2CO3, 10% formaldehyde)
  • Farmer’s reducer (10% sodium thiosulfate, 0.5% potassium ferrocyanide)
  • Glass slide

4.4.3.3 Method

  1. Place sections on a drop of D.W. on to a glass slide.
  2. Stretch the section and let the section dry at r.t.
  3. Add a drop of 5% AgNO3 on to the sections and incubate at R.T. for 5~10min in the humid box.
  4. Rinse 3 times with D.W. for 1 min at R.T.
  5. Add a drop of Sodium Carbonate-formaldehyde on to the sections and incubate at R.T. for 2min.
  6. Rinse 3 times with D.W. for 1 min at R.T.
  7. Add a drop of Farmer’s reducer on to the sections and incubate at R.T. for 30sec.
  8. Rinse 3 times with D.W. for 1 min at R.T.

4.4.3.4 Reference

Krutsy M (1963) Methods for demonstration of some calcium compounds in histologycal sections. Acta Histochem 15: 189-191


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Last-modified: 2019-04-04 (Thu) 14:18:41