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4.4.4 TRAP staining of sections

Yoshiyuki Nemoto
Tokyo Institute of Technology

4.4.4.1 General information

Mature osteoclast has a tartrate-resistant acid phosphatase(TRAP) enzymatic activity.

4.4.4.2 Reagents and equipments

  • Reaction solution:
    1.5mM naphthol AS-MX phosphate, 0.5mM Fast Red Violet LB salt, 20mM L(+)-tartrate in 0.1M acetate buffer (pH 5.2).
  • glass slides

4.4.4.3 Method

  1. Place sections on a drop of D.W. on to a glass slide.
  2. Stretch the section and let the section dry at r.t.
  3. Section is incubated with a drop of reaction solution at 37ºC or r.t. for 1hr in the humid box.
  4. Stop reaction by transferring the section into D.W. and rinse with D.W.

4.4.4.4 Reference

Nakano Y, Toyosawa S, Takano Y (2004). Eccentric localization of osteocytes expressing enzymatic activities, protein, and mRNA signals for type 5 tartrate-resistant acid phosphatase (TRAP). J Histochem Cytochem 52 (11): 1475-1482.


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Last-modified: 2019-04-04 (Thu) 14:18:41