breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 309,381 C→T D216N (GAT→AAT)  ecpB ← putative fimbrial chaperone EcpB
RA 309,985 T→A G14G (GGA→GGT ecpB ← putative fimbrial chaperone EcpB
RA 321,260 A→T intergenic (+179/‑348) rclR → / → ykgE DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
JC JC 1,995,620 IS5 (–) +4 bp intergenic (‑261/‑32) uvrY ← / → yecU DNA‑binding transcriptional activator UvrY/protein YecU
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 354596 354659 64 117 [0] [31] 32 prpE/codB propionyl‑CoA synthetase/cytosine transporter
* * ÷ NC_000913 361657 362203 547 100 [0] [0] 94 [lacA]–[lacY] [lacA],[lacY]
* * ÷ NC_000913 3423891–3424232 3424452–3424238 7–562 32 [31] [31] 32 rrlD 23S ribosomal RNA
* * ÷ NC_000913 3883253 3883537 285 32 [31] [31] 33 dnaA chromosomal replication initiator protein DnaA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 19796 =NA (NA)100 (1.080) 61/584 0.2 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 361656 0 (0.000)coding (205/612 nt) lacA galactoside O‑acetyltransferase
* ? NC_000913 70062 =108 (1.170)95 (1.110)
+22 bp
57/542 0.2 52.3% intergenic (‑14/‑325) araB/araC ribulokinase/DNA‑binding transcriptional dual regulator AraC
?NC_000913 = 367520 79 (0.850)intergenic (‑10/+67) lacI/mhpR DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional activator MhpR
* ? NC_000913 = 7126587 (0.940)117 (1.260) 60/586 0.2 72.9% coding (879/879 nt) araC DNA‑binding transcriptional dual regulator AraC
?NC_000913 = 354595 0 (0.000)intergenic (+3/‑327) prpE/codB propionyl‑CoA synthetase/cytosine transporter
* ? NC_000913 = 258675NA (NA)93 (1.000) 59/586 0.2 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 362204 = 0 (0.000)coding (976/1254 nt) lacY lactose permease
* ? NC_000913 1207790 =28 (0.300)73 (0.830) 42/554 0.8 72.5% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 29 (0.330)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780525 (0.270)44 (0.500) 33/554 1.4 62.6% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 29 (0.330)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)149 (1.630) 89/578 0.0 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein