| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 309,381 | C→T | D216N (GAT→AAT) | ecpB ← | putative fimbrial chaperone EcpB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 309,381 | 0 | C | T | 98.9% | 313.8 / NA | 92 | D216N (GAT→AAT) | ecpB | putative fimbrial chaperone EcpB |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (40/51); total (41/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCCAGCCAGATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑CACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAGTG‑GCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACT > NC_000913/309087‑309675 | gTCCAGCCAGATAAAAGCGCTGCCGATTCGCGGATCGTCCTGTCGACCCTGGCTACCGGCTAGATGAATATAGAGCTGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGTGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TAccc < 2:484435/299‑1 (MQ=255) gATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTg < 1:602013/300‑1 (MQ=255) gATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCAAAA‑‑Gt > 1:426393/1‑299 (MQ=255) aGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAAccc < 1:608975/300‑1 (MQ=255) ggagcggcagggagaccagggagaacggagagaGGAATAGAGAGCGGGACGCGCAGGACGGCCTGGAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCGGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCGTGCAGGCCTGGGGAGAACCGGCGTAAAGGCATGGTCGGTATCCATCGGCACAGCTATCTATTGCACGGGAATGAACTTAC‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGc < 2:4564/271‑1 (MQ=255) aTCGTCCTGGCGACCCTGGCTACCGTCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAg < 1:292915/300‑1 (MQ=255) aTCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAg < 2:525562/300‑1 (MQ=255) gTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCNGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAgt > 2:558283/1‑298 (MQ=255) gTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAgtgt > 1:309943/1‑300 (MQ=255) ggCGACCCTTGCTACCGGCTAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCCGACTGAAGGCCTGCGGAATAACCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGTGGAGAACCGTCGTAAAGGCATTGTCGTTATCCAGCTGCACAGCTATCTACTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAcg < 2:168597/300‑1 (MQ=255) ggCGACCCTGGCTACCGTCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAATGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAC‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAcg < 2:168613/300‑1 (MQ=255) ggCGACCCTGGCTACCGGCGAGATGAATATAGAGCTGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGCAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAcg < 2:717657/300‑1 (MQ=255) ggCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAcg < 1:116282/300‑1 (MQ=255) gACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAcgcg < 1:457537/299‑1 (MQ=255) aCCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACANCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAcgc > 1:550440/1‑297 (MQ=255) aCCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCATGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTACTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCATAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAcgc > 2:124240/1‑297 (MQ=255) aCCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTACTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCCTTGTTATCCGCAGTGTCCAcgc > 2:383082/1‑297 (MQ=255) gCTACCGGCTAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAac < 2:309943/300‑1 (MQ=255) gagaTGAATATAAAGCGGGACACTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACt < 2:403644/300‑1 (MQ=255) gaTGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGATAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACtt > 1:77791/1‑299 (MQ=255) gaTGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACtt > 1:77763/1‑299 (MQ=255) aaTATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGccc > 2:327175/1‑299 (MQ=255) aaTATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCAACCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcac > 2:326497/1‑297 (MQ=255) agCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCAAGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTCAAACGACGCGACTTGCCCGGCATCa > 2:355805/1‑299 (MQ=255) gggACGCTCATGCCGTNCTGAAGCGCCTGACTGAANGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATCTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGANTTGCCCGGCATCAGgt < 1:648966/299‑1 (MQ=255) gggACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGgt < 1:465060/299‑1 (MQ=255) cGCTCATGCCGTCCTGAAGCGCCTGACTGCAGGCCTGCAGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAACAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCCCAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCCACCCGTCTTTTGTTATCTGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATACGGTAGTaa > 1:311905/1‑300 (MQ=255) cGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACACCACCTATCTGCTGCGCACTTATATCAGCAGCGCTGGCATCAGGCTAAACAAACAAAAAGACCATACCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGCCACGTAGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCAAAA‑‑GTTCaa > 2:314444/1‑232 (MQ=255) cTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCAACTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAATTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAAtt > 2:509542/1‑300 (MQ=255) tCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATtc < 1:527311/300‑1 (MQ=255) gTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGt < 1:665645/234‑1 (MQ=255) gTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGt > 2:665645/1‑234 (MQ=255) gTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATcc > 1:499727/1‑239 (MQ=255) gTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTac > 1:570870/1‑300 (MQ=255) tGAAGCGCCTGGCGGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTacac < 2:155330/298‑1 (MQ=255) tGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTacac < 1:118054/298‑1 (MQ=255) tgactgaAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCAGTACTTTGTTATCCGCAGTGTACACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACAGtt > 2:454244/1‑300 (MQ=255) ggAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTGT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCa < 1:19460/300‑1 (MQ=255) ggAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATCCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCa < 1:623236/300‑1 (MQ=255) ggAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TAACCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCa > 2:767574/1‑300 (MQ=255) ggAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTGCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTGT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCa < 1:19444/300‑1 (MQ=255) gAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCaa > 1:113094/1‑300 (MQ=255) gAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCa < 1:20303/299‑1 (MQ=255) ataatCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCaa > 1:457912/1‑298 (MQ=255) aCCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAANTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAgg < 1:546909/297‑1 (MQ=255) aCCTATTTGCTGCGCACTTATGTCAGCAGCACTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCATTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGNACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAgg < 2:244032/297‑1 (MQ=255) tgcGCACTTATGTCATCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGTTAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAg < 2:34901/300‑1 (MQ=255) gcgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGc < 1:681592/300‑1 (MQ=255) cTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGNCTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGc > 2:604425/1‑300 (MQ=255) tATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCNGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCAt > 1:167905/1‑300 (MQ=255) tATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCAt > 1:476047/1‑300 (MQ=255) tATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTACTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGAAGCGACTTGCCAGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGATTTCCGGCAAGGTCCGTAGGc > 2:400076/1‑280 (MQ=255) tCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCNTTGTCGTTATCCATCTGCACAGCTATCTATTTCACGTGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTcc < 2:609079/300‑1 (MQ=255) gcgcTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTAtt > 2:761285/1‑300 (MQ=255) aTCGGGCTGAACGAACAAAAAGACCATACCGAATGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAggg < 2:218795/300‑1 (MQ=255) gggCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑Gc > 1:566663/1‑300 (MQ=255) acgaacAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCACCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGc < 2:514930/300‑1 (MQ=255) gaacAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGc < 2:514556/298‑1 (MQ=255) cAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACt > 1:711320/1‑300 (MQ=255) aGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGTGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAg < 1:316009/300‑1 (MQ=255) aGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAg < 1:316167/300‑1 (MQ=255) gACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGt < 1:447470/300‑1 (MQ=255) aTGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGGAAATGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGTTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGt < 1:516013/299‑1 (MQ=255) aaaCTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAGAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGACTGAACTTATTCACC‑TGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAGTTCTCTTTAAACTCCTTAACGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCTGCAGCGTCACATTTCCTGTATTTGTCAGGGA‑GCCGttagtat > 2:413903/1‑271 (MQ=255) aaaCTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTACTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGAc > 2:413919/1‑300 (MQ=255) aCTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGa < 1:288435/300‑1 (MQ=255) tttCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGcga > 1:293002/1‑300 (MQ=255) ggggATAACTGTCGTAAAGGCATTGTCGTGATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGTTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCCGACGAGGGGCGACGACCAGAAt < 2:77791/300‑1 (MQ=255) ggggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGNCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAAt < 1:557263/300‑1 (MQ=255) ggggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAAt < 2:77763/300‑1 (MQ=255) ggggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGNCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAAt > 1:163106/1‑300 (MQ=255) ggggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGCAGTAATTCTCTTTACACTcc < 2:393607/172‑1 (MQ=255) ggggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGCAGTAATTCTCTTTACACTcc > 1:393607/1‑172 (MQ=255) gaACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGc > 1:444025/1‑300 (MQ=255) gtcgtAAATGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCGGCCAAA‑‑GTGCAACCCGTACTTTTTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGTTAGTAATTCTCTTTACACTCCTTACCGt < 2:311905/169‑1 (MQ=255) gtcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGAt > 1:493837/1‑299 (MQ=255) gtcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGAt > 2:151951/1‑299 (MQ=255) tGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAg < 1:628244/300‑1 (MQ=255) tGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTNATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAg < 1:558283/300‑1 (MQ=255) ttATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAGTG‑Gcc > 2:751602/1‑300 (MQ=255) tGCACAGCTATCTATTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTGATTCTCTTTACACTCCTTACCGTTTGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAGTG‑Gcc < 2:132991/291‑1 (MQ=255) ctatctatTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAGTG‑GCCACAGCGCTGCgg < 2:293002/296‑1 (MQ=255) tatctatTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGTTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAGTG‑GCCACAGCGCTGCGGTTGGc < 2:684744/300‑1 (MQ=255) tatctatTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGt < 2:608575/134‑1 (MQ=255) tatctatTTCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGt > 1:608575/1‑134 (MQ=255) ttCACGGTAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGTTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAGTG‑GCCACAGCGCTGCGGTTGGCATTATcg < 2:384152/300‑1 (MQ=255) ttCACGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAGTG‑GCCACAGCGCTGCGGTTGGCATTATcg < 1:383082/300‑1 (MQ=255) aCGGGAATGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGNGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAGTG‑GCCACAGCGCTGCGGTTGGCATTATCgcgc < 2:550440/300‑1 (MQ=255) tGAACTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAGTG‑GCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCAt > 1:753232/1‑300 (MQ=255) aCTTAT‑TACCCTGCCAAC‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCATGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTCATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTACTGTATTTGTCAGGGA‑GCCGTTGGTGTACTGAAAGTGGTAGTTCGCCTGACGCGGGGCGACGACAAGAATGGTGCCGATGCGGGCGGAA‑GCAGTG‑GCCACAGCGCTGCGGTTGGCATTCTCGCGCTGCGCATCaa > 2:223231/1‑299 (MQ=255) aCTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTTCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCATTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAGTT‑GCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCAc < 2:521500/300‑1 (MQ=255) cTTAT‑TACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAGTG‑GCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACt > 1:680772/1‑300 (MQ=255) ttat‑taCCCTGCCAAANGGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGACGCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGCAGAGGGGCGACGACCAGACTGGTGCCAATGCGGGCGGAACGCAGTGCNCCCCAGCGCTGCGGtt > 1:636162/1‑280 (MQ=255) | GTCCAGCCAGATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAT‑CACCCTGCCAAA‑‑GTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGA‑GCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCGGAA‑GCAGTG‑GCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACT > NC_000913/309087‑309675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |