breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 121 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_012971875,1910GT62.5% 18.2 / 31.3 32pseudogene (4/1116 nt)ECD_RS04405replication endonuclease
*NC_0129713,483,9750CT60.2% 72.7 / 123.3 103G800G (GGC→GGTrhsBrhs element protein RhsB
*NC_0129713,483,9770GA60.2% 84.9 / 121.7 103W801* (TGG→TAG) ‡rhsBrhs element protein RhsB
*NC_0129713,483,9780GC59.8% 81.2 / 144.4 103W801C (TGG→TGC) ‡rhsBrhs element protein RhsB
*NC_0129713,483,9820TG58.5% 85.9 / 121.2 106S803A (TCA→GCA) rhsBrhs element protein RhsB
*NC_0129713,484,9500TC56.7% 18.3 / 197.2 150T1125T (ACT→ACCrhsBrhs element protein RhsB
*NC_0129713,484,1330AC56.4% 73.0 / 143.3 118Y853S (TAT→TCT) rhsBrhs element protein RhsB
*NC_0129713,484,0860CT56.2% 57.9 / 171.3 128Y837Y (TAC→TATrhsBrhs element protein RhsB
*NC_0129713,484,0750TA55.7% 59.1 / 214.3 123S834T (TCC→ACC) rhsBrhs element protein RhsB
*NC_0129713,484,1300AT55.4% 47.5 / 216.3 121Q852L (CAG→CTG) rhsBrhs element protein RhsB
*NC_0129713,484,8040AG52.6% 31.2 / 205.0 136T1077A (ACC→GCC) ‡rhsBrhs element protein RhsB
*NC_0129713,484,8060CG51.9% 29.5 / 232.3 135T1077T (ACC→ACG) ‡rhsBrhs element protein RhsB
*NC_0129713,484,2340AG50.8% 7.1 / 200.8 128S887G (AGC→GGC) rhsBrhs element protein RhsB
*NC_0129711,403,4150GA47.3% 13.9 / 263.3 169R26Q (CGG→CAG) ECD_RS07135YdfR family protein
*NC_0129713,483,0280TA46.4% 32.8 / 233.6 125L485M (TTG→ATG) ‡rhsBrhs element protein RhsB
*NC_0129713,483,0300GA46.4% 18.6 / 177.6 125L485L (TTG→TTA) ‡rhsBrhs element protein RhsB
*NC_0129711,403,4100GC45.4% 68.0 / 270.7 175A24A (GCG→GCCECD_RS07135YdfR family protein
*NC_0129711,403,4230AG45.1% 58.4 / 245.2 173K29E (AAA→GAA) ECD_RS07135YdfR family protein
*NC_0129713,482,9900AG44.9% 37.2 / 165.1 128N472S (AAC→AGC) rhsBrhs element protein RhsB
*NC_0129713,483,0930TC44.5% 44.0 / 165.4 119T506T (ACT→ACCrhsBrhs element protein RhsB

Marginal new junction evidence (lowest skew 10 of 22 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_012971 = 73957958 (0.600)13 (0.180) 11/438 4.1 21.3% noncoding (76/76 nt) ECD_RS03630 tRNA‑Lys
?NC_012971 740008 = 53 (0.740)intergenic (+1/‑132) ECD_RS03645/ECD_RS03650 tRNA‑Lys/tRNA‑Lys
* ? NC_012971 = 73978583 (0.860)12 (0.170) 10/438 4.4 17.3% noncoding (76/76 nt) ECD_RS03640 tRNA‑Lys
?NC_012971 740008 = 53 (0.740)intergenic (+1/‑132) ECD_RS03645/ECD_RS03650 tRNA‑Lys/tRNA‑Lys
* ? NC_012971 = 3510532102 (1.060)11 (0.140) 10/474 4.8 12.4% intergenic (‑358/‑382) dinQ/arsR damage‑inducible type I toxin DinQ/As(III)‑sensing metalloregulatory transcriptional repressor ArsR
?NC_012971 3510764 = 73 (0.940)intergenic (‑590/‑150) dinQ/arsR damage‑inducible type I toxin DinQ/As(III)‑sensing metalloregulatory transcriptional repressor ArsR
* ? NC_012971 = 73929099 (1.030)6 (0.080) 6/438 5.9 9.4% noncoding (76/76 nt) ECD_RS03620 tRNA‑Lys
?NC_012971 739580 = 42 (0.590)intergenic (+1/‑51) ECD_RS03630/ECD_RS03635 tRNA‑Lys/tRNA‑Val
* ? NC_012971 = 65661567 (0.700)8 (0.110) 6/434 5.9 13.8% intergenic (‑1/+15) ECD_RS03205/ECD_RS03210 tRNA‑Met/tRNA‑Gln
?NC_012971 657008 = 51 (0.720)intergenic (‑2/+379) ECD_RS03225/asnB tRNA‑Met/asparagine synthase B
* ? NC_012971 = 3643247112 (1.160)10 (0.110) 8/534 6.1 9.0% coding (1822/4851 nt) ECD_RS18090 YadA‑like family protein
?NC_012971 3643503 = 102 (1.170)coding (2078/4851 nt) ECD_RS18090 YadA‑like family protein
* ? NC_012971 = 93060463 (0.650)5 (0.060) 5/472 6.8 9.5% noncoding (118/136 nt) ECD_RS22710 RtT sRNA
?NC_012971 930960 = 45 (0.580)noncoding (69/88 nt) ECD_RS04720 tRNA‑Ser
* ? NC_012971 = 65638075 (0.780)6 (0.070)
+45 bp
5/500 7.1 8.5% intergenic (‑2/+36) ECD_RS03195/ECD_RS03200 tRNA‑Gln/tRNA‑Gln
?NC_012971 656930 = 77 (0.800)noncoding (77/77 nt) ECD_RS03225 tRNA‑Met
* ? NC_012971 = 25178500 (0.000)9 (0.090)
+TT
6/586 7.5 16.3% intergenic (+31/‑53) sseA/ECD_RS25300 3‑mercaptopyruvate sulfurtransferase/IS3‑like element ISEcB1 family transposase
?NC_012971 2519624 = 93 (0.970)intergenic (+364/‑302) ECD_RS25300/timP IS3‑like element ISEcB1 family transposase/small toxic inner membrane protein TimP
* ? NC_012971 739932 =85 (0.880)6 (0.060)
+GG
6/586 7.5 6.8% noncoding (1/76 nt) ECD_RS03645 tRNA‑Lys
?NC_012971 = 2397543 80 (0.830)noncoding (76/76 nt) ECD_RS11975 tRNA‑Val