breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 184 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0129713,483,0300GA59.3% 80.6 / 140.8 118L485L (TTG→TTA) ‡rhsBrhs element protein RhsB
*NC_0129713,483,0930TC58.7% 61.0 / 137.9 110T506T (ACT→ACCrhsBrhs element protein RhsB
*NC_0129713,482,9900AG58.0% 74.1 / 152.5 132N472S (AAC→AGC) rhsBrhs element protein RhsB
*NC_0129713,483,0280TA58.0% 83.5 / 194.7 120L485M (TTG→ATG) ‡rhsBrhs element protein RhsB
*NC_0129713,484,6420GC54.1% 4.6 / 153.2 98E1023Q (GAA→CAA) rhsBrhs element protein RhsB
*NC_0129713,484,2340AG53.1% 3.3 / 166.8 114S887G (AGC→GGC) rhsBrhs element protein RhsB
*NC_0129713,484,1330AC51.7% 18.1 / 166.6 118Y853S (TAT→TCT) rhsBrhs element protein RhsB
*NC_0129713,484,1300AT50.8% ‑6.5 / 236.9 120Q852L (CAG→CTG) rhsBrhs element protein RhsB
*NC_0129713,723,4260AC50.8% 28.9 / 270.1 193T8T (ACA→ACCECD_RS18465DUF957 domain‑containing protein
*NC_0129713,723,3020GA50.7% ‑4.4 / 300.8 204V134I (GTC→ATC) ECD_RS18460DUF5983 family protein
*NC_0129713,484,8060CG50.4% 74.5 / 249.3 135T1077T (ACC→ACG) ‡rhsBrhs element protein RhsB
*NC_0129713,723,2650GA50.3% 16.6 / 289.0 185K121K (AAG→AAAECD_RS18460DUF5983 family protein
*NC_0129713,723,2930TC50.3% 22.1 / 314.1 196C131R (TGT→CGT) ECD_RS18460DUF5983 family protein
*NC_0129713,484,8040AG50.0% 65.0 / 213.2 136T1077A (ACC→GCC) ‡rhsBrhs element protein RhsB
*NC_0129713,484,0860CT49.2% ‑3.1 / 191.6 122Y837Y (TAC→TATrhsBrhs element protein RhsB
*NC_0129713,723,2710GA49.0% 25.5 / 296.4 196L123L (CTG→CTAECD_RS18460DUF5983 family protein
*NC_0129713,484,5960AG49.0% 27.3 / 140.3 96R1007R (CGA→CGGrhsBrhs element protein RhsB
*NC_0129713,723,2470CT48.9% 7.1 / 272.8 175Y115Y (TAC→TATECD_RS18460DUF5983 family protein
*NC_0129713,723,2740CG48.5% 51.2 / inf 196G124G (GGC→GGGECD_RS18460DUF5983 family protein
*NC_0129713,484,0750TA48.3% 23.0 / 225.0 118S834T (TCC→ACC) rhsBrhs element protein RhsB

Marginal new junction evidence (lowest skew 10 of 32 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_012971 550553 =0 (0.000)44 (0.440) 18/588 4.1 100% pseudogene (4/364 nt) ECD_RS02680 prophage tail fiber N‑terminal domain‑containing protein
?NC_012971 = 788130 0 (0.000)pseudogene (6/1155 nt) ECD_RS23105 prophage tail fiber N‑terminal domain‑containing protein
* ? NC_012971 = 278574764 (0.630)10 (0.140) 10/424 4.6 15.9% intergenic (+4/‑30) ECD_RS13895/ECD_RS13900 tRNA‑Met/tRNA‑Met
?NC_012971 2785968 = 60 (0.830)intergenic (+5/+69) ECD_RS13905/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase AmiC
* ? NC_012971 = 73978584 (0.830)11 (0.150) 10/438 4.7 16.0% noncoding (76/76 nt) ECD_RS03640 tRNA‑Lys
?NC_012971 740008 = 53 (0.710)intergenic (+1/‑132) ECD_RS03645/ECD_RS03650 tRNA‑Lys/tRNA‑Lys
* ? NC_012971 = 74000973 (0.720)10 (0.130) 8/434 5.4 12.7% intergenic (+2/‑131) ECD_RS03645/ECD_RS03650 tRNA‑Lys/tRNA‑Lys
?NC_012971 740218 = 84 (1.130)intergenic (+3/‑275) ECD_RS03650/nadA tRNA‑Lys/quinolinate synthase NadA
* ? NC_012971 = 65661547 (0.460)10 (0.130) 7/434 5.8 15.6% intergenic (‑1/+15) ECD_RS03205/ECD_RS03210 tRNA‑Met/tRNA‑Gln
?NC_012971 657008 = 74 (1.000)intergenic (‑2/+379) ECD_RS03225/asnB tRNA‑Met/asparagine synthase B
* ? NC_012971 = 3723604102 (1.010)17 (0.170)
+CACTCATCATC
8/568 6.9 14.8% intergenic (+4/‑81) ECD_RS18465/ECD_RS18470 DUF957 domain‑containing protein/DUF4942 domain‑containing protein
?NC_012971 3723605 = 102 (1.010)intergenic (+5/‑80) ECD_RS18465/ECD_RS18470 DUF957 domain‑containing protein/DUF4942 domain‑containing protein
* ? NC_012971 = 3644070103 (1.020)7 (0.070) 7/580 7.4 6.8% coding (2645/4851 nt) ECD_RS18090 YadA‑like family protein
?NC_012971 3644368 = 90 (0.910)coding (2943/4851 nt) ECD_RS18090 YadA‑like family protein
* ? NC_012971 739215 =118 (1.170)6 (0.080) 4/438 7.4 8.5% noncoding (1/76 nt) ECD_RS03620 tRNA‑Lys
?NC_012971 = 739503 42 (0.560)intergenic (+2/‑1) ECD_RS03625/ECD_RS03630 tRNA‑Val/tRNA‑Lys
* ? NC_012971 = 25178500 (0.000)3 (0.040)
+88 bp
3/414 7.7 7.2% intergenic (+31/‑53) sseA/ECD_RS25300 3‑mercaptopyruvate sulfurtransferase/IS3‑like element ISEcB1 family transposase
?NC_012971 2519706 = 110 (1.090)intergenic (+446/‑220) ECD_RS25300/timP IS3‑like element ISEcB1 family transposase/small toxic inner membrane protein TimP
* ? NC_012971 3136294 =98 (0.970)5 (0.060)
+53 bp
4/484 7.9 5.8% intergenic (+392/+116) yhaC/rnpB YhaC family protein/RNase P RNA component class A
?NC_012971 4046335 = 100 (0.990)intergenic (‑131/+144) yijF/gldA DUF1287 domain‑containing protein/bifunctional L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase