breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 37 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913442,4710CA40.6% 121.6 / 10.6 97S308Y (TCC→TAC) thiItRNA uridine 4‑sulfurtransferase
*NC_000913190,8430CA40.0% 109.4 / 10.2 95intergenic (+244/‑14)rpsB/tsf30S ribosomal subunit protein S2/protein chain elongation factor EF‑Ts
*NC_0009131,351,4630CA37.2% 112.5 / 10.5 78E100* (GAG→TAG) ycjDDUF559 domain‑containing protein YcjD
*NC_0009131,820,7630GT36.8% 112.2 / 20.2 87S151Y (TCC→TAC) chbCN,N'‑diacetylchitobiose‑specific PTS enzyme IIC component
*NC_0009133,911,5330CA36.6% 175.3 / 24.1 134intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009132,913,0850GT35.9% 168.2 / 14.4 106L189L (CTC→CTArelAGDP/GTP pyrophosphokinase
*NC_0009132,288,4180GT35.8% 206.9 / 29.1 134pseudogene (497/2525 nt)yejOadhesin‑like autotransporter YejO
*NC_00091358,0650CA35.6% 153.5 / 19.1 104P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009131,427,9920GT33.6% 197.7 / 10.0 122P245P (CCC→CCAinsH5IS5 family transposase and trans‑activator
*NC_00091385,6510GT32.8% 175.0 / 16.2 131E8* (GAG→TAG) ilvIacetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
*NC_000913332,9750CA32.1% 165.4 / 11.4 106S202* (TCG→TAG) pdeLDNA‑binding transcriptional regulator/c‑di‑GMP phosphodiesterase PdeL
*NC_0009132,117,6520GT31.0% 167.6 / 11.5 87L251L (CTC→CTAwcaKcolanic acid biosynthesis pyruvyl transferase WcaK
*NC_000913847,9550GA30.1% 182.7 / 53.4 123A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_0009131,217,5280CT29.6% 154.0 / 95.9 108C67C (TGC→TGTpdeGputative c‑di‑GMP phosphodiesterase PdeG
*NC_000913141,8490CA29.4% 220.5 / 27.3 119P140Q (CCG→CAG) hpthypoxanthine phosphoribosyltransferase
*NC_0009134,360,9650CA29.2% 306.0 / 22.8 147E324* (GAG→TAG) cadCDNA‑binding transcriptional activator CadC
*NC_0009133,741,8710GT29.1% 191.7 / 18.3 103S221Y (TCC→TAC) plaRDNA‑binding transcriptional repressor PlaR
*NC_0009133,054,8860GT28.0% 190.7 / 22.8 100Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_0009133,431,3960CA27.6% 231.7 / 14.5 127R7I (AGA→ATA) tsaCL‑threonylcarbamoyladenylate synthase
*NC_0009131,812,5090GT27.2% 281.4 / 20.6 136L208L (CTC→CTAydjOprotein YdjO

Marginal new junction evidence (lowest skew 10 of 34 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 78122280 (0.820)15 (0.210) 12/438 4.0 19.3% noncoding (76/76 nt) lysY tRNA‑Lys
?NC_000913 781445 = 66 (0.910)intergenic (+1/‑132) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
* ? NC_000913 = 69674170 (0.720)14 (0.200) 11/434 4.2 20.2% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 59 (0.820)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 265341989 (0.910)13 (0.150) 12/540 5.0 13.1% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 91 (1.020)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 = 460616681 (0.830)8 (0.120) 7/412 5.4 11.8% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 63 (0.930)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 2518116105 (1.080)7 (0.100) 7/428 5.6 9.1% noncoding (1/76 nt) alaX tRNA‑Ala
?NC_000913 2518232 = 63 (0.890)intergenic (‑1/‑235) alaW/yfeC tRNA‑Ala/DNA‑binding transcriptional dual regulator YfeC
* ? NC_000913 = 69650697 (1.000)9 (0.110)
+45 bp
8/500 6.0 10.7% intergenic (‑2/+36) glnX/glnV tRNA‑Gln/tRNA‑Gln
?NC_000913 697057 = 80 (0.820)noncoding (77/77 nt) metT tRNA‑Met
* ? NC_000913 = 235769098 (1.010)9 (0.100) 7/540 6.8 9.5% coding (787/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357874 = 82 (0.920)intergenic (+71/+168) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 = 3269938111 (1.140)7 (0.080) 7/546 6.9 5.9% intergenic (+336/+278) yhaC/rnpB uncharacterized protein YhaC/RNase P catalytic RNA component
?NC_000913 3270266 = 120 (1.330)noncoding (327/377 nt) rnpB RNase P catalytic RNA component
* ? NC_000913 = 78144689 (0.910)4 (0.060) 4/434 7.1 6.3% intergenic (+2/‑131) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
?NC_000913 781655 = 54 (0.750)intergenic (+3/‑430) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 364788699 (1.020)6 (0.070) 6/540 7.3 6.3% intergenic (‑98/‑177) dinQ/agrA membrane toxin DinQ/small RNA AgrA
?NC_000913 3648317 = 88 (0.990)noncoding (24/82 nt) agrB small regulatory RNA AgrB