breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 34 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,040,6230GA78.8% 127.9 / 43.9 85noncoding (107/120 nt)rrfA5S ribosomal RNA
*NC_0009134,166,8560GA51.2% 14.3 / 59.8 41noncoding (198/1542 nt)rrsB16S ribosomal RNA
*NC_0009131,001,0940GT36.6% 118.7 / 22.4 93E627* (GAG→TAG) elfCputative fimbrial usher protein ElfC
*NC_0009132,990,4800CA35.7% 163.2 / 27.3 126intergenic (‑120/‑788)yqeK/ygeGprotein YqeK/TPR repeat‑containing putative chaperone YgeG
*NC_0009133,911,5330CA33.3% 179.8 / 23.0 129intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_00091358,0650CA32.6% 138.7 / 16.7 92P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009134,074,5720CA31.6% 117.2 / 16.0 81P278P (CCC→CCAyihV6‑deoxy‑6‑sulfofructose kinase
*NC_00091312,1520GT31.4% 191.7 / 13.0 124intergenic (‑366/‑11)yaaI/dnaKDUF2541 domain‑containing protein YaaI/chaperone protein DnaK
*NC_0009133,791,1790GT29.7% 175.6 / 11.2 101P56Q (CCG→CAG) tdhthreonine dehydrogenase
*NC_000913847,9550GA29.5% 204.2 / 45.9 129A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_0009131,466,4380TG28.9% 54.7 / 22.7 47pseudogene (1047/2518 nt)ydbAputative outer membrane protein, N‑terminal fragment
*NC_0009132,466,6330CA28.4% 176.9 / 14.5 102P30H (CCT→CAT) intSprophage CPS‑53 integrase
*NC_0009132,722,8380GT28.3% 222.1 / 13.1 113E38* (GAG→TAG) pssAphosphatidylserine synthase
*NC_0009132,365,8400TG27.8% 176.0 / 10.8 109intergenic (‑220/‑70)ais/arnBputative lipopolysaccharide core heptose(II)‑phosphate phosphatase/UDP‑4‑amino‑4‑deoxy‑L‑arabinose aminotransferase
*NC_000913442,0250CA27.6% 151.3 / 13.0 87L159L (CTC→CTAthiItRNA uridine 4‑sulfurtransferase
*NC_0009131,286,8700CA27.6% 97.5 / 29.1 58S54S (TCC→TCAychSputative uncharacterized protein YchS
*NC_0009134,360,9650CA27.6% 320.5 / 20.9 156E324* (GAG→TAG) cadCDNA‑binding transcriptional activator CadC
*NC_000913141,8490CA27.1% 234.4 / 10.2 108P140Q (CCG→CAG) hpthypoxanthine phosphoribosyltransferase
*NC_0009131,502,7700TC27.0% 102.4 / 15.1 74I105T (ATA→ACA) ydcLDUF3313 domain‑containing lipoprotein YdcL
*NC_0009133,741,8710GT26.5% 222.6 / 17.0 117S221Y (TCC→TAC) plaRDNA‑binding transcriptional repressor PlaR

Marginal new junction evidence (lowest skew 10 of 19 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1411899 =110 (1.200)15 (0.180) 12/536 4.1 12.0% coding (50/936 nt) ttcA tRNA cytosine(32) 2‑sulfurtransferase TtcA
?NC_000913 = 1434958 119 (1.430)intergenic (‑552/+227) ynaM/uspF protein YnaM/universal stress protein F
* ? NC_000913 = 78122258 (0.630)7 (0.100) 7/436 4.7 13.5% noncoding (76/76 nt) lysY tRNA‑Lys
?NC_000913 781653 = 47 (0.690)intergenic (+1/‑432) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 69674155 (0.600)10 (0.150) 7/432 4.7 19.2% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 44 (0.660)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 69646979 (0.860)10 (0.110)
+TAGAAT
8/576 5.6 13.1% noncoding (36/75 nt) glnX tRNA‑Gln
?NC_000913 696802 = 57 (0.620)noncoding (29/75 nt) glnW tRNA‑Gln
* ? NC_000913 = 460616691 (1.000)4 (0.060) 4/410 5.8 6.2% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 57 (0.890)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 69650681 (0.890)6 (0.080)
+45 bp
5/498 6.2 8.8% intergenic (‑2/+36) glnX/glnV tRNA‑Gln/tRNA‑Gln
?NC_000913 697057 = 65 (0.710)noncoding (77/77 nt) metT tRNA‑Met
* ? NC_000913 = 80586272 (0.790)6 (0.070) 6/574 6.4 8.1% intergenic (+98/+136) ybhJ/ybhC putative hydratase YbhJ/outer membrane lipoprotein YbhC
?NC_000913 805955 = 65 (0.730)intergenic (+191/+43) ybhJ/ybhC putative hydratase YbhJ/outer membrane lipoprotein YbhC
* ? NC_000913 = 265350296 (1.050)4 (0.050) 4/520 6.9 4.3% intergenic (‑47/‑353) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
?NC_000913 2653825 = 92 (1.140)intergenic (‑370/‑30) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 = 235771385 (0.930)6 (0.070)
+C
5/586 6.9 6.1% coding (810/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357910 = 99 (1.080)intergenic (+107/+132) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 = 3469040106 (1.160)4 (0.050) 4/548 7.1 3.9% coding (814/2694 nt) chiA endochitinase
?NC_000913 3469344 = 101 (1.190)coding (510/2694 nt) chiA endochitinase