breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 47 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,671,5310GT35.6% 119.5 / 12.0 87E103* (GAG→TAG) hcaB2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase
*NC_0009133,911,5330CA34.5% 218.9 / 16.1 139intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009131,351,4630CA34.3% 97.2 / 13.3 70E100* (GAG→TAG) ycjDDUF559 domain‑containing protein YcjD
*NC_0009133,741,8710GT31.1% 156.4 / 14.0 90S221Y (TCC→TAC) plaRDNA‑binding transcriptional repressor PlaR
*NC_0009132,990,4800CA31.0% 276.7 / 17.5 158intergenic (‑120/‑788)yqeK/ygeGprotein YqeK/TPR repeat‑containing putative chaperone YgeG
*NC_000913834,6740CA30.5% 174.8 / 10.3 95L535L (CTC→CTAdinGATP‑dependent DNA helicase DinG
*NC_00091312,1520GT30.4% 152.8 / 13.1 102intergenic (‑366/‑11)yaaI/dnaKDUF2541 domain‑containing protein YaaI/chaperone protein DnaK
*NC_0009131,812,5090GT30.4% 258.4 / 13.2 135L208L (CTC→CTAydjOprotein YdjO
*NC_0009134,096,4220CA30.2% 208.6 / 12.3 119E343* (GAG→TAG) rhaBrhamnulokinase
*NC_0009134,360,9650CA29.1% 363.6 / 22.6 175E324* (GAG→TAG) cadCDNA‑binding transcriptional activator CadC
*NC_0009134,136,4670AC29.1% 151.4 / 10.9 79E120D (GAA→GACyijEcystine exporter
*NC_0009131,423,7940CA28.7% 300.3 / 11.2 160H5N (CAT→AAT) trkGK(+) transporter TrkG
*NC_0009131,756,3530CA28.5% 223.8 / 13.9 130S219Y (TCC→TAC) pykFpyruvate kinase 1
*NC_0009133,768,4350CA28.3% 377.9 / 15.5 194Q87K (CAG→AAG) yibGtetratricopeptide‑like domain‑containing protein YibG
*NC_000913952,4530GT28.3% 214.7 / 10.2 120P368T (CCG→ACG) pflBpyruvate formate‑lyase
*NC_0009132,477,2650AC28.3% 172.7 / 16.3 99L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009132,767,4450CA27.9% 172.7 / 16.0 86intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009132,185,8500GT27.8% 254.7 / 15.0 126intergenic (‑54/‑67)rcnR/rcnADNA‑binding transcriptional repressor RcnR/Ni(2(+))/Co(2(+)) exporter
*NC_0009131,097,1180CA27.5% 54.5 / 28.1 40intergenic (+289/+447)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_000913710,8300GT27.3% 249.4 / 13.0 139intergenic (‑105/+105)uof/fldARyhB‑regulated fur leader peptide/flavodoxin 1

Marginal new junction evidence (lowest skew 10 of 28 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 696469102 (0.940)18 (0.190)
+36 bp
13/518 4.8 18.4% noncoding (36/75 nt) glnX tRNA‑Gln
?NC_000913 696941 = 80 (0.740)intergenic (‑2/+22) glnU/leuW tRNA‑Gln/tRNA‑Leu
* ? NC_000913 = 78132266 (0.610)11 (0.120) 9/512 6.0 14.1% intergenic (+100/‑47) lysY/lysZ tRNA‑Lys/tRNA‑Lys
?NC_000913 781811 = 77 (0.820)intergenic (+159/‑274) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 2357730127 (1.170)8 (0.090) 8/510 6.4 7.7% coding (827/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357926 = 82 (0.880)intergenic (+123/+116) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 = 409435067 (0.620)9 (0.100) 7/508 6.8 13.6% intergenic (‑78/+373) rhaD/rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
?NC_000913 4094719 = 57 (0.610)intergenic (‑447/+4) rhaD/rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
* ? NC_000913 = 128690670 (0.650)11 (0.110) 8/550 6.8 14.5% coding (198/276 nt) ychS putative uncharacterized protein YchS
?NC_000913 1287557 = 64 (0.630)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 781577 =71 (0.660)15 (0.140)
+GAAG
8/582 7.1 15.5% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 95 (0.880)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 271761NA (NA)10 (0.090) 8/584 7.1 58.9% noncoding (1221/1221 nt) IS30 repeat region
?NC_000913 = 4640419 7 (0.070)coding (18/141 nt) yjjY uncharacterized protein YjjY
* ? NC_000913 = 290422592 (0.850)7 (0.070) 6/530 7.4 6.5% intergenic (+851/+522) ygcE/queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
?NC_000913 2904409 = 119 (1.220)intergenic (+1035/+338) ygcE/queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
* ? NC_000913 = 3651580142 (1.310)8 (0.070) 7/590 7.6 5.3% pseudogene (290/746 nt) yhiS putative uncharacterized protein YhiS
?NC_000913 3652036 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 696506115 (1.060)5 (0.050)
+45 bp
5/500 7.6 5.4% intergenic (‑2/+36) glnX/glnV tRNA‑Gln/tRNA‑Gln
?NC_000913 697057 = 91 (0.840)noncoding (77/77 nt) metT tRNA‑Met