breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 51 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913339,9790GA79.0% 65.5 / 13.4 38V71M (GTG→ATG) yahHputative uncharacterized protein YahH
*NC_0009131,298,0920GT50.0% 6.3 / 180.1 122N10K (AAC→AAAadhEfused acetaldehyde‑CoA dehydrogenase and iron‑dependent alcohol dehydrogenasealdehyde/alcohol dehydrogenase AdhE
*NC_0009131,298,0910CG49.2% 12.3 / 217.4 122A11P (GCA→CCA) adhEfused acetaldehyde‑CoA dehydrogenase and iron‑dependent alcohol dehydrogenasealdehyde/alcohol dehydrogenase AdhE
*NC_0009134,166,8920CT46.7% 6.4 / 41.9 30noncoding (234/1542 nt)rrsB16S ribosomal RNA
*NC_0009133,054,8940GT39.0% 105.3 / 45.1 101P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_0009134,136,4670AC38.6% 75.6 / 18.4 57E120D (GAA→GACyijEcystine exporter
*NC_0009133,054,8860GT36.6% 138.3 / 38.9 102Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_0009131,756,3530CA36.0% 168.9 / 19.6 111S219Y (TCC→TAC) pykFpyruvate kinase 1
*NC_0009131,427,9920GT35.6% 147.3 / 23.7 116P245P (CCC→CCAinsH5IS5 family transposase and trans‑activator
*NC_0009134,296,2680TC35.1% 37.4 / 33.1 38intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009133,911,5330CA33.9% 175.2 / 20.3 121intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009131,334,9660AC33.7% 251.7 / 34.5 169intergenic (+137/‑182)cysB/ymiADNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA
*NC_00091358,0650CA33.0% 182.2 / 24.5 103P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009134,296,1910AC30.6% 34.8 / 18.2 36intergenic (+397/+245)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009132,326,7130CA30.3% 168.9 / 11.8 99P202Q (CCG→CAG) atoBacetyl‑CoA acetyltransferase
*NC_0009133,602,7050TG30.0% 90.0 / 21.9 60R15R (AGA→CGA) ftsEcell division protein FtsE
*NC_0009132,477,2650AC29.4% 194.3 / 34.4 102L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009132,990,4800CA28.5% 430.9 / 34.7 246intergenic (‑120/‑788)yqeK/ygeGprotein YqeK/TPR repeat‑containing putative chaperone YgeG
*NC_000913538,0750AG28.1% 131.9 / 17.6 82D148G (GAT→GGT) ybbWputative allantoin transporter
*NC_0009132,490,2400GT28.0% 227.7 / 12.9 126intergenic (‑54/+16)yfdV/oxcputative transport protein YfdV/oxalyl‑CoA decarboxylase

Marginal new junction evidence (lowest skew 10 of 22 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3722075NA (NA)32 (0.320) 26/596 3.0 25.3% noncoding (1443/1443 nt) IS150 repeat region
?NC_000913 = 4354449 95 (0.950)coding (269/1518 nt) lysU lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
* ? NC_000913 3720633 =NA (NA)20 (0.200) 16/596 5.0 17.4% noncoding (1/1443 nt) IS150 repeat region
?NC_000913 4354447 = 95 (0.950)coding (271/1518 nt) lysU lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
* ? NC_000913 = 4606166105 (1.050)7 (0.100) 6/420 6.5 9.1% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 66 (0.940)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 69674172 (0.720)6 (0.080) 6/442 6.7 9.7% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 58 (0.790)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 78122252 (0.520)7 (0.090) 6/446 6.8 11.7% noncoding (76/76 nt) lysY tRNA‑Lys
?NC_000913 781653 = 67 (0.900)intergenic (+1/‑432) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 2288919 =NA (NA)9 (0.090) 8/598 7.6 8.3% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 3455529 = 100 (1.000)intergenic (‑131/‑49) gspA/gspC Type II secretion system protein GspA/Type II secretion system protein GspC
* ? NC_000913 = 439243766 (0.660)4 (0.050) 4/442 7.8 5.9% intergenic (+2/‑35) glyV/glyX tRNA‑Gly/tRNA‑Gly
?NC_000913 4392661 = 78 (1.060)intergenic (+3/‑231) glyY/yjeV tRNA‑Gly/uncharacterized protein YjeV
* ? NC_000913 = 128690653 (0.530)6 (0.060) 6/558 8.1 9.8% coding (198/276 nt) ychS putative uncharacterized protein YchS
?NC_000913 1287557 = 61 (0.650)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 2357730125 (1.250)7 (0.080) 5/518 8.2 6.4% coding (827/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357926 = 98 (1.130)intergenic (+123/+116) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 1096961 =72 (0.720)4 (0.050) 3/438 8.4 6.0% intergenic (+132/+604) ycdU/serX uncharacterized protein YcdU/tRNA‑Ser
?NC_000913 = 1097503 72 (0.980)intergenic (+674/+62) ycdU/serX uncharacterized protein YcdU/tRNA‑Ser