Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,602,7050TG30.0% 90.0 / 21.9 60R15R (AGA→CGA) ftsEcell division protein FtsE
Reads supporting (aligned to +/- strand):  ref base T (27/15);  new base G (0/18);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.43e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTT  >  NC_000913/3602421‑3603003
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                          
ggCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGc                                                                                                                                                                                                                                                                                             <  1:520538/300‑1 (MQ=255)
 gCACCGNCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCNGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCCGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGGCGACCACCGAGATAAGcc                                                                                                                                                                                                                                                                                            <  2:661596/300‑1 (MQ=255)
 gCACCGNCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCNGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCACCTGTCGACCACCGAGATAAGcc                                                                                                                                                                                                                                                                                            <  2:658529/300‑1 (MQ=255)
 gCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGcc                                                                                                                                                                                                                                                                                            <  1:639676/300‑1 (MQ=255)
                    ggATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCATGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCTTGAAGGGTATGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGc                                                                                                                                                                                                                                                                         <  2:287577/300‑1 (MQ=255)
                    ggATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGACTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCTGCTGCATATGGAACGTAACGCCCTGAAGAATACGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGc                                                                                                                                                                                                                                                                         <  2:11508/300‑1 (MQ=255)
                                 ctCGTAGACAGTCCGGTCCATCAGTCGATGGTGATCCTGGAAAATCATGCCAACCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCAAAAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCATTGCTGACACGGTCAAAGAGAATCAtcggtca                                                                                                                                                                                                                                                            >  2:118514/2‑294 (MQ=255)
                                 ctCGTAGACAGTACGGTCCATCCGTCGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCATGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCAACAGATCAGCTTAAGGAGGGTACTTTTCCCTGCGCCTGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGCACGTAACGCCCTGCAGCGCCTGTCTCCCCCCGAGATAAGCCTTGCTGAAACGTTCAAAGAGAGTCATTGTTac                                                                                                                                                                                                                                                            >  2:513837/2‑299 (MQ=255)
                                 ctCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCATTGCTGACATGTTCACAGCGAATCATTGTTaa                                                                                                                                                                                                                                                            >  1:801072/2‑300 (MQ=255)
                                 aTCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCNCGGCGCTGGGCCGCTCAATCCCACAGNTCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCATTGCTGACATGTTCAAAGCGAATCAGTGTTaa                                                                                                                                                                                                                                                            >  2:529325/1‑300 (MQ=255)
                                   cGTAGGCAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTTGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCTTGATGTCATGGCCGCTAAGCCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCATAAACGCCATCTCACCCTGCTGCATATTGAACTTAACGCTTTGAAGGATATGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATc                                                                                                                                                                                                                                                          <  2:344589/300‑1 (MQ=255)
                                   cGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTNGCGGCGCAGAAACGGAACTTCACGGTTGTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATTGAACTTAACGCCCTGAAGCGTACGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATc                                                                                                                                                                                                                                                          <  2:583866/300‑1 (MQ=255)
                                   cGTAGACAGTACGGTCCAGCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTNGCGGCGCAGAAACGGAACTTCACGGTGTTTCAGACGCTTGATGTCATGGCTGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGTAGGGTACTTTTCCCTGCGCCGGAATGACCGTTCAGAAACCCCACCTCACCCGCCTGCACATCCAACCTAACGCCTTGAATCATACGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATc                                                                                                                                                                                                                                                          <  2:583282/300‑1 (MQ=255)
                                    gTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATTGAACGTAACGCCCTGCAGCGCATGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATcc                                                                                                                                                                                                                                                         <  2:481493/300‑1 (MQ=255)
                                        aCAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGNGCATAAACGGAACTTCACGGTTTTTCATACGCGTGATGTCATGGCCGCTAAACCAGATTTCCCCTGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCAGTCAGAAACTCCACCTCACCCTGCTGCATATTGAACCTACCTCCCTGCATCATCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCtctc                                                                                                                                                                                                                                                     <  2:597043/300‑1 (MQ=255)
                                         cAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGNAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCg                                                                                                                                                                                                                                                    >  1:535422/1‑300 (MQ=255)
                                         cAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCg                                                                                                                                                                                                                                                    >  1:284391/1‑300 (MQ=255)
                                         cAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCATTGCTGACATGTTCAAAGCGAATCATTGTTCATCCTCTCg                                                                                                                                                                                                                                                    >  2:787426/1‑300 (MQ=255)
                                                gTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCaaaa                                                                                                                                                                                                                                             >  1:69479/1‑300 (MQ=255)
                                                  ccATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAg                                                                                                                                                                                                                                           <  1:59637/300‑1 (MQ=255)
                                                   cATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCNCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGt                                                                                                                                                                                                                                          >  1:538387/1‑300 (MQ=255)
                                                   cATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCNCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTAGGGCAAAAAGt                                                                                                                                                                                                                                          >  1:538465/1‑300 (MQ=255)
                                                                tGATCCTTGAAAATCATGCCAATCTGGCGGCGCATGAACGGAACTTCACGGTTTTTCAGATGCTTGATGTCATTGCCGCTAAACCAGATTTTCCCGGCGCTGTGCCGCTCAATCCCACAAATCAACTTCATTNGTGTACTTTTCCCTGCGCCGGAATGACCCGTCATAAACGCCATCTCACCCTGCTACATATCGAACTTAACGCCCTTAAGCACACGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAgt                                                                                                                                                                                                                             <  2:179935/300‑1 (MQ=255)
                                                                tGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCTTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCTAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAgt                                                                                                                                                                                                                             <  1:227958/300‑1 (MQ=255)
                                                                tGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAgt                                                                                                                                                                                                                             <  1:260931/300‑1 (MQ=255)
                                                                ggatccgtaaaaaTCATGCCAATCTGGCGGCGCAGAGACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCACTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCACCTCACCCGGCTGCATATGGAACGTAACGCCCTGAAGAATATGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAgt                                                                                                                                                                                                                             <  2:27381/291‑1 (MQ=255)
                                                                                                                        cAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCNGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCATCTGTCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCgg                                                                                                                                                                     <  2:690306/300‑1 (MQ=255)
                                                                                                                        cAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCgg                                                                                                                                                                     <  1:431170/300‑1 (MQ=255)
                                                                                                                        cAGACGCGTAATGTCATGGCCGCTAAACCAGATTTTCCCGGCACTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCTCCGGAATGACCGGTCAGAAACACCATCTCACCCTGCTGCATATGGAACGCAACGCCCTTAAGCACACGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCgg                                                                                                                                                                     <  2:219481/300‑1 (MQ=255)
                                                                                                                              cgTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATTAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTAACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCGGACATGTTCAAAGCGAGTCATTGTTAATCCTCTCGGGCAAAAAGTGTATATATAAAGTCACCCGCCTTAAACGGACGCAAACCATCAATACGTTAGACGACAATAATGTAGCGGCTAggg                                                                                                                                                               >  2:49676/1‑300 (MQ=255)
                                                                                                                               gTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCTGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCGCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGa                                                                                                                                                              <  2:473736/300‑1 (MQ=255)
                                                                                                                               gTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTCCCCTGCACCGGAATGACCGATCATAAACACCATCTCACCCGTCTGCATACTTAACTCAACGCCCTGCAGCATCCGTCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGa                                                                                                                                                              <  2:243795/300‑1 (MQ=255)
                                                                                                                                    gTCATGTCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATNAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCCCACCCTGCTGCATATTGAACTTAACGCCCTGCAGCGTCTGTCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTANACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCa                                                                                                                                                         <  2:748659/300‑1 (MQ=255)
                                                                                                                                                                gcgcTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTAccc                                                                                                                             >  1:98367/1‑300 (MQ=255)
                                                                                                                                                                gcgcTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGAACGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCACATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTAccc                                                                                                                             >  1:297158/1‑300 (MQ=255)
                                                                                                                                                                               aTCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCgccgtgc                                                                                                              >  1:647072/1‑300 (MQ=255)
                                                                                                                                                                                       gATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGt                                                                                                      >  1:490130/1‑300 (MQ=255)
                                                                                                                                                                                         tCAGCTNCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCANCTCACCCGGCTGCATATGGAACTTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGttt                                                                                                    <  2:655728/300‑1 (MQ=255)
                                                                                                                                                                                           aGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCg                                                                                                  >  1:387068/1‑300 (MQ=255)
                                                                                                                                                                                            gCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGt                                                                                                 >  1:482182/1‑300 (MQ=255)
                                                                                                                                                                                             cTTCATGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGTAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGtt                                                                                                <  2:515628/300‑1 (MQ=255)
                                                                                                                                                                                             cTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGtt                                                                                                >  1:166242/1‑300 (MQ=255)
                                                                                                                                                                                                                 cTGCGCCGGAATGACCATTCAGAAACCCCATCTCACCCGGCTGCATATTGAACGNAACGCCCTGCAGCACCTGTCTCCCACCTAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGcc                                                                            <  2:598922/300‑1 (MQ=255)
                                                                                                                                                                                                                        ggAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCt                                                                     >  1:268725/1‑300 (MQ=255)
                                                                                                                                                                                                                                 ggTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAAc                                                            >  1:657436/1‑300 (MQ=255)
                                                                                                                                                                                                                                 attCAGAAACGCCACCTCACCCGGCGGCATCTTGAACTTAACGCCCTGCAGTATATGTCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAAc                                                            <  2:425495/298‑1 (MQ=255)
                                                                                                                                                                                                                                  gTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACa                                                           >  1:643474/1‑300 (MQ=255)
                                                                                                                                                                                                                                      taccctccATCTCACCCTTCTGCATATTGACCTTAACTCCCTGCAGTGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGttt                                                       <  2:115071/294‑1 (MQ=255)
                                                                                                                                                                                                                                          cGCCACCTCACCCGGCTGCATATGGAACGTAACGCCCTGCATCACCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGcc                                                   <  2:513681/300‑1 (MQ=255)
                                                                                                                                                                                                                                                    cccGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCTAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACcg                                         <  1:315687/300‑1 (MQ=255)
                                                                                                                                                                                                                                                        gCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGtt                                     >  1:781277/1‑300 (MQ=255)
                                                                                                                                                                                                                                                           gCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTg                                  >  1:27318/1‑300 (MQ=255)
                                                                                                                                                                                                                                                             atatGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCCCCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGcc                                >  1:727580/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                   aaCGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGc                          <  1:672758/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                    aCGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCt                         >  1:696592/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                        aaCGCCCTGCATCATCTGTCGCCCACCTAGATAAGCCTTTCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCNCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCa                     <  2:705065/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                        aaCGCCCTGCAGCGCCTGTCGCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAANGTAGCGGATAGGGATACCAAACTGGTNAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCa                     <  2:546051/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                           gCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCa                  >  1:764136/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                    cgcCTGTCTCCCACCTAGATAAGCCTTGCTGACATGTTCAAAGTGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTAGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGc         <  2:324114/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     gcCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCa        >  1:783417/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                           ctcCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACtt  <  1:388656/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           ctcCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACtt  <  2:79270/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                          
GGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTT  >  NC_000913/3602421‑3603003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑