breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 258,756 G→T pseudogene (81/331 nt) crl → RNA polymerase holoenzyme assembly factor Crl
MC JC 260,173 Δ13,782 bp IS5‑mediated proB[ykfC] 19 genes
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 578,244 A→G L46L (CTA→CTG rzpD → putative prophage endopeptidase RzpD
RA 635,355 G→A R8* (CGA→TGA) 
N405N (AAC→AAT
ybdM ←
ybdN ←
ParB‑like nuclease domain‑containing protein YbdM
putative PAPS reductase/DUF3440 domain‑containing protein YbdN
MC JC 676,374 Δ11,477 bp IS5‑mediated [ybeQ]gltI [ybeQ], ybeR, djlB, ybeT, ybeU, djlC, hscC, rihA, gltL, gltK, gltJ, sroC, gltI
RA 696,582 C→T noncoding (35/75 nt) glnV ← tRNA‑Gln
RA 771,504 C→T A16V (GCG→GTG)  cydA → cytochrome bd‑I subunit 1
RA 811,531 G→A G384D (GGT→GAT) 
V4I (GTT→ATT) 
bioF →
bioC →
8‑amino‑7‑oxononanoate synthase
malonyl‑acyl carrier protein methyltransferase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,705,108 G→A G54R (GGG→AGG)  cnu → H‑NS‑ and StpA‑binding protein
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
JC JC 2,108,146 IS1 (–) +8 bp coding (177‑184/1104 nt) glf ← UDP‑galactopyranose mutase
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,718,740 T→G N2K (AAT→AAG trxC → reduced thioredoxin 2
RA 2,774,080 G→T V255F (GTC→TTC)  yfjW → uncharacterized protein YfjW
RA 2,817,997 A→G intergenic (‑137/+62) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
MC JC 3,678,269 Δ42,364 bp IS150‑mediated [yhjG]hokA 36 genes
JC JC 4,050,808 IS2 (–) +5 bp intergenic (‑43/‑224) yihA ← / → csrC GTP‑binding protein YihA/small regulatory RNA CsrC
RA 4,132,697 G→A E28K (GAG→AAG)  metF → 5,10‑methylenetetrahydrofolate reductase
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,566,642 G→A S202L (TCG→TTG)  yjiN ← DUF445 domain‑containing protein YjiN

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423765–3424232 3424492–3424238 7–728 38 [37] [37] 39 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1978503 =NA (NA)79 (0.740) 58/598 0.5 58.1% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 3888846 57 (0.530)coding (117/1416 nt) tnaA tryptophanase
* ? NC_000913 3186096 =NA (NA)78 (0.730) 55/598 0.7 53.8% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 3654624 = 67 (0.630)intergenic (+97/‑59) slp/dctR starvation lipoprotein/putative DNA‑binding transcriptional regulator DctR
* ? NC_000913 = 3187426NA (NA)67 (0.630) 51/596 0.8 50.8% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 3654628 65 (0.610)intergenic (+101/‑55) slp/dctR starvation lipoprotein/putative DNA‑binding transcriptional regulator DctR
* ? NC_000913 3583428 =NA (NA)57 (0.530) 45/598 1.2 48.7% noncoding (1/768 nt) IS1 repeat region
?NC_000913 3888838 = 60 (0.560)coding (109/1416 nt) tnaA tryptophanase