Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,997	A→G	intergenic (‑137/+62)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,997	0	A	G	100.0%	199.0 / NA	60	intergenic (‑137/+62)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/44);  total (16/44)

TGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTT  >  NC_000913/2817701‑2818291                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                        ttgcgcGACGTTATCCTTTCTAGTGTCTACGGTTGAGACTGCTATCTCTTCGATACCGTCATTACTGAAGGTTACGGAGAATATGGTGCATCTTGGAGGATTCGAACCTCCGAGTGCGCGGTTCGGAGCCGAGTACTCTATCTAGCTG‑CGCTACGGATGCATCTGGAAAACTTATNCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGGGATGGTGCATCCGGGAGGATTATTCTCCTTGCGGCTCGCCCTTCGGGNCGTTGCTAAAGCAACGTTGTCATCCCTGGTg                                                                                                                                                                                                                                                                                                      <  2:601095/298‑1 (MQ=18) tGGCGCAACGTTATCCTTCCTGTTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTg                                                                                                                                                                                                                                                                                                      <  1:785557/300‑1 (MQ=255) tGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTg                                                                                                                                                                                                                                                                                                      <  2:839278/300‑1 (MQ=255) tGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTg                                                                                                                                                                                                                                                                                                      <  1:270120/300‑1 (MQ=255)   gcgcAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGcc                                                                                                                                                                                                                                                                                                    <  1:863438/300‑1 (MQ=255)        acgctATCCTTCCTGTTGCCTGCGGTTAATACCGCTATATCGTCGAGACCTCCATTGCTGAAGGTTACGGAGAATATCGTGCATCCGGGAGAATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCGATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCACATCAATTAAGAGATGGTGCATCCGTGAGGATTACGCGCCTTGCTGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCgg                                                                                                                                                                                                                                                                                               <  2:63736/296‑1 (MQ=18)        aCGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAATTCATTCCACTGCTGCTATTGATCCCACTACCAAAGCCATATCAATTAAGAGATGGTTCCCCCGGGAGGATTCCCCCCCCTGCGGCTCGCCCCTCGGGCCGTTTCTCCAGCAACATTATCATCAATGGTGCCTGCgg                                                                                                                                                                                                                                                                                               >  2:497250/1‑300 (MQ=17)        aCGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTAATGCTGATATTGCTACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCACTGGTGCCTGCgg                                                                                                                                                                                                                                                                                               >  1:524691/1‑300 (MQ=255)        aCGTTATCCTTACTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCCTACGGCTCGCCCTTCGGGCCGTTGCTAAAGCCACGTTATCATCCCTGGTGCCTGCgg                                                                                                                                                                                                                                                                                               >  1:122611/1‑300 (MQ=255)                             gCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCNTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAATAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                                                                                                                                                                                                                                                                          <  2:196819/300‑1 (MQ=255)                                gTTAATACCGCCATCTCGTCGATACCTTCATTGCTGAAGGTTGCGGAGGATATGGTGCATCCGGGAAGATGCGAAACTCCTACCGCGCGGTTCGTAGCCGAGTACGCTGGCCA‑CTGGAGCTACGGATGCATCGGGAAAACTTATTTTACTGCTGAGAGTAATATAGCTACTAAAACTAAATCGATTAAGAGATGGGGCATCCGGGAGGATTACGCGCCTTGCAGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATCCCGCTACCTCTTCGATAc                                                                                                                                                                                                                                                                       <  1:395763/300‑1 (MQ=16)                                       ccGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATACAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGTAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAtt                                                                                                                                                                                                                                                                <  1:217880/300‑1 (MQ=255)                                              ctctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAg                                                                                                                                                                                                                                                         <  1:349936/300‑1 (MQ=255)                                                       aCCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACNCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTACTTCCGCTACCTCTTCAATACCTTCATCGCTGAAGATTTGGgag                                                                                                                                                                                                                                                >  1:587642/1‑300 (MQ=255)                                                               tgcggANGGTTACTGGGAATATGGTGCATCCTGGAGGATTCGAACCTCCGACCGNTCGGTTCTTAGCCGAGTACGCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACGTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGt                                                                                                                                                                                                                                       <  2:700353/296‑1 (MQ=18)                                                               tGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGt                                                                                                                                                                                                                                       <  1:189502/300‑1 (MQ=255)                                                                 cTGAAGGTTACTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGTAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGc                                                                                                                                                                                                                                     <  1:180606/300‑1 (MQ=255)                                                                 cTGAAGGTTACCGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCTACCGCTCGGTTCGTAGCCGGGTACTCTAGCCAACTG‑AGCTACGGATACATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGTGAGGAGTACTCGCCTTGCTGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGc                                                                                                                                                                                                                                     <  2:312698/300‑1 (MQ=18)                                                                      ggTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCg                                                                                                                                                                                                                                <  1:367588/300‑1 (MQ=255)                                                                          actgagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTATCCGAGTACTCTATCCAGCGG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGTCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGggag                                                                                                                                                                                                                            <  2:803237/297‑1 (MQ=255)                                                                          actgagaATAGGGTGCATCCGGGAGGAGTCGAACCTCCGACCGCTCGGTTCGTACCCGAGTACTCTATCCAGCGG‑AGCTACGGATGCATCGGGAAAACTTACTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACGCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGggag                                                                                                                                                                                                                            <  2:804561/297‑1 (MQ=18)                                                                           cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGggagg                                                                                                                                                                                                                           <  1:827973/300‑1 (MQ=255)                                                                                             cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGc                                                                                                                                                                                                         <  1:267711/300‑1 (MQ=255)                                                                                                    aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                                                                                                                  <  1:683445/300‑1 (MQ=255)                                                                                                         acccTCCGGCCGCTCGTTTCTTCGCCTATTACTCTATCCATCTG‑AGCTACGGATGCGTCTGGAAAACCTATTCTACTGCTGATATGGATACCGCTACTAAAGCCAGATCAATTAAGAAATGGTGCATACGGGAGGAGTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                                                                                                                                                             <  2:343543/298‑1 (MQ=18)                                                                                                         aacctacgatcgcgtggTTCCTAGCCGAGTACTCTGTCCAGCGG‑AGCTACGGATGCATCGGGAAAACATATTCTACTGCTTACATTGATACCGCTACTAAAGCCATATTAATTAGGAGATGGTGTATCCGTGAGGATGACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                                                                                                                                                             <  2:818563/285‑1 (MQ=18)                                                                                                         aaCCTCCTACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                                                                                                                                                             <  1:695390/300‑1 (MQ=255)                                                                                                         aaCCTCCGACCGCTTGGTTCTTAGCCGAGTACTCTAGCCAGCTG‑AGCTACGGATGCATCGGGAAAACGTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAGGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                                                                                                                                                             <  2:837619/300‑1 (MQ=255)                                                                                                         aaCCTCCGACCGCTCGGTTCTTATCCGATGACTCTATCCATTTG‑GGCTACGGATGCGTCGGGAAAGCTTATTTTACTGCTGATAGTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCGAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                                                                                                                                                             <  1:688932/300‑1 (MQ=17)                                                                                                         aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAGACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCNATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                                                                                                                                                             <  1:625837/300‑1 (MQ=255)                                                                                                         aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                                                                                                                                                             <  1:191608/300‑1 (MQ=255)                                                                                                                gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTAAGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAc                                                                                                                                                                                      <  1:229845/300‑1 (MQ=255)                                                                                                                       cGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGAGATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTNCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc                                                                                                                                                                               <  1:630423/300‑1 (MQ=255)                                                                                                                       cGGTTCGTAGCCGAGTACTCTAGCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCTGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc                                                                                                                                                                               <  1:183119/300‑1 (MQ=255)                                                                                                                       aagagacaggCCGAGTACTCTATCCAGCTG‑AGCTACGAATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTGCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc                                                                                                                                                                               <  2:318413/291‑1 (MQ=255)                                                                                                                        agTTCGTAGCCGAGGACGCTATCCAGCTG‑GGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATAGTGATACCGCTACTAAAGCCATATCAATTAGGGGATGGTGCATCCGTGAGGATTACGCGCCTTGCGGTTCGCCCTTCGTGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCa                                                                                                                                                                              <  2:470968/299‑1 (MQ=18)                                                                                                                         gTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGCGTACTCTGTCCAg                                                                                                                                                                             >  1:87661/1‑300 (MQ=255)                                                                                                                         gTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCATTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                                                                                                                             >  2:547181/1‑300 (MQ=255)                                                                                                                         gTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTAATGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTTGGAGAGCG‑TGGTGCATCGGGGAGGGTTGGAGCCTCCGCCTGCTTGGTTCGCAGCCGAgcatttcatccga                                                                                                                                                                             >  1:328241/1‑288 (MQ=18)                                                                                                                            cGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                                                                                                                          <  1:557352/300‑1 (MQ=255)                                                                                                                                gCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATccctgtctctt                                                                                                                                                                      >  1:320559/1‑291 (MQ=255)                                                                                                                                      tACTCTAGCCGGCCG‑AGCTACGGATGCATCGGGAAAACTTATCCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCTGGAGGATTACTCGCCTTGCTGCTCGCCCTTCGTGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGAt                                                                                                                                                                <  1:34022/300‑1 (MQ=18)                                                                                                                                       aCTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCCTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCATGGTGCCTGCGGTTAATCCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATG‑TGGTGCATCGGGGAGGATTCGAACCTCCGCCCGTTCGGTTTGTATCCGAGTACTCTATCCAGCTGAgttcagggtg                                                                                                                                                               >  1:108281/1‑291 (MQ=18)                                                                                                                                          ctATCCAGCTG‑AGCTACGGATGCATCGGGAAGACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTAGTAACCTTGCGAATTGCACTTAGGGCCGTTTCTAAAATACCGTTATCATCCACGGTGCCTGCGGCTCATTCCGCTAACTCTTCGATCACTTCATTGCTGAAGGTTTCTGAGAATG‑TGGTGCATCAGGGGGGAATCGAACTTCCGACTGCTAGGTTCGTAGCTGAGTATTCGACCCAGCTGAGCTACGGATGCAt                                                                                                                                                            >  1:274101/1‑300 (MQ=12)                                                                                                                                                    g‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAGCGGGaggtgg                                                                                                                                                  >  1:262552/1‑295 (MQ=255)                                                                                                                                                                         ggaCTTATTCTGCTGCTGAGATTGGTACCGCTACTAAAGTCATATCAGTTGAGGGATGGTGCATCCTGGAGGATGACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCTGGAGGATTCGAACCTCCGACCACTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGAt                                                                                                                              <  2:317413/298‑1 (MQ=18)                                                                                                                                                                            cTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCATTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAcc                                                                                                                           >  1:454372/1‑300 (MQ=255)                                                                                                                                                                                      tgctgAGATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATACGGGAGGATTACGCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATGCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAg                                                                                                                 <  1:278902/300‑1 (MQ=255)                                                                                                                                                                                            tatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTACTCCCGCCACCTCTTCGATACCTTCATTGCTGAAGATTTCTGA‑AATGGTGTTGCCTCTGGTAAACTTCTAACCTCCGACCGTTCGGCTCTTAGCCGAGTGCTCTATCCAGCTGAGCTACGGATGCATGGGGGAGGTTATTTTACTGCTGCTGGTGATACCGCTACTAAAGCCatat                                                                                                           >  1:47448/1‑300 (MQ=17)                                                                                                                                                                                            tatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCatat                                                                                                           >  1:36117/1‑300 (MQ=255)                                                                                                                                                                                                            aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTg                                                                                           <  1:685521/300‑1 (MQ=255)                                                                                                                                                                                                                  atatCGATTAAGAGAGGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGTGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAATAGATGGTGCATCCg                                                                                     <  1:260878/300‑1 (MQ=255)                                                                                                                                                                                                                                      catccttgagaattacgctccTTGCCGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTGCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGAc                                                                 <  1:800455/281‑1 (MQ=17)                                                                                                                                                                                                                                                     ttCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCTGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAATAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                  <  1:167168/299‑1 (MQ=255)                                                                                                                                                                                                                                                     cTCGCCTTGCGGCTCGCCCTTCTGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCGACCTCTTCGATACCTGCATTGCTGAAGATTTCGGAGGATA‑TGGGGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTGGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATGTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                  <  1:182725/300‑1 (MQ=18)                                                                                                                                                                                                                                                               ggCTTGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAATGCTGATCTTGATACCGCTCCTACAACCATATCCCATCGGAGAGGGTGCATCCGGTAGGCTTCGAACCTCCGCCCACTCGGTTCGTCGCCGAGTAatct                                        >  1:758163/1‑300 (MQ=17)                                                                                                                                                                                                                                                                   cGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc                                    <  1:564541/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      ccTTCGGGCCGTGGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCTACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc                                 <  2:449500/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 ttGCTAAAGCAACGTCATCATCCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGANCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGa                      <  1:606236/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     tcCCTGGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAGGTTTATTTTACTGCTGATATTGATATAACTACTAAAGCCATATCACGTAAGAGATGGTGCATCCGGGAGGATTCGAACCCACGCCAGCTCGGTCCGTAGCCGAGTACTCTATAAAGCTGAGCTACGGATGCACCGGGagacccatctc  >  2:426720/1‑290 (MQ=17)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                        TGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTG‑AGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATA‑TGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTT  >  NC_000913/2817701‑2818291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑