breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 41 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,750,2170GA76.7% 181.9 / 54.8 86D164D (GAC→GATgrpEnucleotide exchange factor GrpE
*NC_000913186,1170GA70.0% 195.9 / 94.7 111A845V (GCC→GTC) glnDprotein‑PII uridylyltransferase/uridylyl‑removing enzyme
*NC_0009131,097,1580CT43.3% 20.6 / 31.7 30intergenic (+329/+407)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009132,767,4450CA33.6% 202.9 / 14.9 110intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009134,296,1910AC33.3% 20.4 / 12.2 24intergenic (+397/+245)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009132,767,4440CA31.8% 213.4 / 11.4 110intergenic (+89/‑266)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009132,073,6130GT31.5% 161.9 / 14.4 89R692L (CGG→CTG) fluself recognizing antigen 43 (Ag43) autotransporter
*NC_0009132,477,2650AC31.2% 210.3 / 41.2 109L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009133,970,2530GT31.1% 187.4 / 17.2 107E41* (GAG→TAG) wecBUDP‑N‑acetylglucosamine 2‑epimerase
*NC_0009133,054,8860GT30.5% 193.0 / 26.1 105Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_0009133,669,7150GT29.7% 188.8 / 17.7 118E42* (GAG→TAG) treFcytoplasmic trehalase
*NC_0009131,097,1180CA29.6% 43.4 / 11.1 27intergenic (+289/+447)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009133,054,8940GT29.5% 177.9 / 27.3 107P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_000913430,2990CA29.2% 193.2 / 11.3 97F265L (TTC→TTAsecFSec translocon accessory complex subunit SecF
*NC_00091312,1520GT29.0% 233.5 / 16.6 131intergenic (‑366/‑11)yaaI/dnaKDUF2541 domain‑containing protein YaaI/chaperone protein DnaK
*NC_0009132,465,0160CA28.9% 217.2 / 14.4 114intergenic (‑9/‑285)mlaA/yfdCintermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA/inner membrane protein YfdC
*NC_0009132,159,4150CG28.8% 133.4 / 17.8 58L344V (CTG→GTG) mdtCmultidrug efflux pump RND permease subunit MdtC
*NC_0009133,032,3070CT28.8% 174.6 / 44.0 111T314M (ACG→ATG) uacTurate:H(+) symporter
*NC_000913186,4080GT28.6% 136.4 / 68.5 84A748E (GCG→GAG) glnDprotein‑PII uridylyltransferase/uridylyl‑removing enzyme
*NC_0009132,990,4800CA28.6% 356.3 / 18.0 182intergenic (‑120/‑788)yqeK/ygeGprotein YqeK/TPR repeat‑containing putative chaperone YgeG

Marginal new junction evidence (lowest skew 10 of 13 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1411899 =129 (1.210)16 (0.160) 15/546 5.0 11.8% coding (50/936 nt) ttcA tRNA cytosine(32) 2‑sulfurtransferase TtcA
?NC_000913 = 1434958 122 (1.250)intergenic (‑552/+227) ynaM/uspF protein YnaM/universal stress protein F
* ? NC_000913 = 265341994 (0.880)12 (0.120) 12/548 5.9 11.4% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 100 (1.020)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 4542682 =166 (1.550)16 (0.150) 11/580 6.6 9.2% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 153 (1.470)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 4542690177 (1.650)10 (0.100) 10/580 6.9 5.8% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 153 (1.470)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 69674189 (0.830)8 (0.100) 6/442 7.0 11.5% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 57 (0.720)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 78122267 (0.630)6 (0.080) 6/446 7.1 10.3% noncoding (76/76 nt) lysY tRNA‑Lys
?NC_000913 781653 = 54 (0.680)intergenic (+1/‑432) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 3648146122 (1.140)4 (0.050) 4/482 8.6 3.9% intergenic (+2/‑148) agrA/agrB small RNA AgrA/small regulatory RNA AgrB
?NC_000913 3648378 = 99 (1.150)intergenic (+3/‑150) agrB/arsR small regulatory RNA AgrB/DNA‑binding transcriptional repressor ArsR
* ? NC_000913 = 252100688 (0.820)3 (0.040) 3/446 8.8 4.5% noncoding (76/76 nt) valU tRNA‑Val
?NC_000913 2521127 = 61 (0.760)intergenic (+1/‑46) valX/valY tRNA‑Val/tRNA‑Val
* ? NC_000913 = 128708464 (0.600)5 (0.050) 5/558 8.9 7.8% noncoding (153/171 nt) rttR small RNA RttR
?NC_000913 1287557 = 59 (0.590)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 346904989 (0.830)4 (0.040) 4/582 9.7 3.9% coding (805/2694 nt) chiA endochitinase
?NC_000913 3469353 = 110 (1.060)coding (501/2694 nt) chiA endochitinase