breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,2680TC45.2% 15.5 / 28.0 32intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009131,097,4370AG44.8% ‑0.4 / 31.1 30intergenic (+608/+128)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009134,171,5570CG44.0% 27.3 / 87.2 50intergenic (+13/‑80)rrlB/rrfB23S ribosomal RNA/5S ribosomal RNA
*NC_0009132,326,4500TG42.1% 49.2 / 11.9 38G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_0009132,228,2780GC31.6% 65.6 / 11.7 38intergenic (‑1008/‑704)yohF/yohPputative oxidoreductase YohF/putative membrane protein YohP
*NC_0009132,434,8820TG31.6% 98.1 / 13.7 58Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
*NC_0009132,185,1990TG30.9% 126.0 / 12.6 69T35P (ACC→CCC) thiMhydroxyethylthiazole kinase
*NC_0009134,622,2500AG30.0% 70.4 / 10.0 40E161G (GAG→GGG) yjjJtoxin YjjJ
*NC_0009131,328,4650TG26.9% 162.8 / 13.8 78T216T (ACA→ACCyciKputative oxidoreductase
*NC_0009132,185,2380AG24.6% 140.3 / 13.8 69S22P (TCC→CCC) thiMhydroxyethylthiazole kinase
*NC_000913803,8690GT23.8% 80.3 / 17.7 42V123L (GTG→TTG) ybhJputative hydratase YbhJ
*NC_000913119,3960CT23.1% 122.0 / 13.9 65S39F (TCT→TTT) ampEprotein AmpE
*NC_0009133,813,2720CT21.3% 105.9 / 12.4 61S181L (TCG→TTG) dfpfused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 78158354 (0.830)3 (0.050) 3/464 5.0 5.8% noncoding (7/76 nt) lysQ tRNA‑Lys
?NC_000913 = 781641 45 (0.710)noncoding (65/76 nt) lysQ tRNA‑Lys
* ? NC_000913 = 78121851 (0.780)3 (0.050) 3/464 5.0 5.6% noncoding (72/76 nt) lysY tRNA‑Lys
?NC_000913 = 781576 52 (0.820)intergenic (+132/‑1) lysZ/lysQ tRNA‑Lys/tRNA‑Lys