| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,434,882 | 0 | T | G | 31.6% | 98.1 / 13.7 | 58 | Y252S (TAC→TCC) | truA | tRNA pseudouridine(38‑40) synthase |
| Reads supporting (aligned to +/- strand): ref base T (6/33); new base G (17/1); total (24/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGACT > NC_000913/2434656‑2435128 | aCCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGcc < 2:458895/251‑1 (MQ=255) ccGTCCTCCGCGACCAGCTCGGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGTCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCTCCGCGACCAGATACAGCCCTTCCGC‑tt < 2:300268/251‑1 (MQ=255) cTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGAt < 2:8778/231‑1 (MQ=255) gcgACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTtg < 2:42155/251‑1 (MQ=255) gACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGA‑TAAAA‑CCCGGCA‑GGGGAATACACCCCGTCCAACTACAACCCTTCCCC‑ttttttccttgct > 2:474149/1‑242 (MQ=255) ctctGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGA‑TCATA‑ACGGGTA‑GGGGAAACCACCGCGACCAGATACAACCCTTCCGC‑CTTTTCCGTTTCTgccccc > 1:14073/1‑248 (MQ=255) tGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGGA‑GA‑TCATA‑CCGGTGC‑GGGGAATTCCCCCCCACCCGATACAGCCCCTCCCC‑tttttcccgtgcttcccccacccgtcttt > 2:381262/1‑226 (MQ=255) ggATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGA‑TCATA‑CCGGTCA‑GGGGAATCCCCCCCGCCCCGATACAACCCTTCCGC‑CTTTTCCCTTGCTGCCGCCAGCGTTCTGTCCTTTTCCGCCCGCac > 2:356938/1‑250 (MQ=255) cGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑ACGGTCA‑GGGGAAACCACCGCGACCAGATACAGCCCTTCCGC‑TTTTTCCGTTGGTGCCGCCCGCGTTCTGTCCTTTGCCGCCCGCAACCCCGCTAt > 1:408041/1‑251 (MQ=255) gAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGA‑GCATA‑ACGGGCACGGGAAATC‑ACCCCGACCAGATACAACCCTTTCGCCTTTTTCCGT‑GCTGCCCCCAGCGGTCTGTCCTTTTCCGCCCGCAActcctccttccaactc > 1:210569/1‑238 (MQ=255) aataaatCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCtctc < 2:96144/251‑1 (MQ=255) atCAGGTCCATTGTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCt < 1:340674/251‑1 (MQ=255) atCAGGTCCATATTTGCCTATGCCTTGTACTCGTAATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCt < 2:343931/251‑1 (MQ=255) ggTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCATGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATT‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGtt < 1:438749/251‑1 (MQ=255) ggTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTGCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGtt < 2:119399/251‑1 (MQ=255) ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGTAGA‑TCATA‑CCGGTCA‑GGGGAAACCCCCGCGCCCCGATACACCCCCTCCGC‑TTTTTCCGTTGCTGCCGCCCCCCCTCTTTCCCTTTCCGCCCGCCACCCTGCTCTCCAGCTCTCCGCCCGGTtgtg > 2:477209/1‑251 (MQ=255) ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGG‑TCATT‑ACGGTCA‑AGGGAATCCACCGCGACCAGATACAACCCCTCCCC‑TTTTTCCGCTGCTGCCCCCCGCGGTCTGTCCTTTGCCGCCAGCAACTCCGCTATCCAGCCCCCCCGCTGGTTGTGGGCAc‑c > 2:358501/1‑251 (MQ=255) tGCCTATGCCTTTTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAc‑c < 2:132194/250‑1 (MQ=255) aTGCCTTGTACGCGTCATCTTTCCGGCTGGAACTGCGTTTGTCACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACt < 2:261450/249‑1 (MQ=255) aTGCCTGGTACTCGGCATCTTTCAGGCTGTAACTACGGTGGCTACAGCCTTAAATGTTCCGATCATTATTCTCGTTAGTCCGCCAGAAAGAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCGTCCGC‑GTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTGTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTTGGCAC‑CGACTTc < 2:388080/251‑1 (MQ=255) tGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGA‑TCATA‑CCCGTCA‑GGGGAATCCACCGCGACCCGAt > 1:300802/1‑150 (MQ=255) tGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGAt < 2:300802/150‑1 (MQ=255) gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGA‑TCATA‑CCGGTCC‑GGGGAATCCACCGCGACCAGCTACAGCCCTTCCCC‑CTTTTCCGTTTCTGCCGCCAGCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTATCCAGCTCTCCCGCCGGTTGTGGGCAC‑CCACTTCCATCAgg > 1:373821/1‑251 (MQ=255) tttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑ACGGTCA‑GGGGAATCCCCCGCGACCAGATACAGCCCCTCCGC‑TTTTGCCGTTTCTGCCGCCCGCGGTCTGTCCTTTGCCGCCCGCAACTCTGCTCTCCAGCTCTCCGGCTGGGTGTGGGGAC‑CGCCTTCCACCAGGCTGCCGACaac > 1:130676/1‑249 (MQ=255) ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGTTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACaa < 1:141414/248‑1 (MQ=255) ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACaa < 1:405133/248‑1 (MQ=255) aCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGATTTTGGA‑GG‑TTAAA‑CCGGGCA‑GGGGAATACAACCCGACCAGATAAAACCCTTTCTC‑TTTTACCGTTTCTTCCCCCACCGGTCTTTCCTTTGACGCCAGCAACTCTGATATCCAACTCTTCGGCTGGGTGTGGGCACACACCTTCC‑TCCGGCAGCCGACAATATTCCTGAccctt > 1:246726/1‑248 (MQ=255) aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGA‑TCATAACCGG‑CA‑GGGGAAACCACCGCGACCAGATACAAACCCTCCCC‑TTTTTCCGTTGCTGCCCCCCGCGTTCTGTCCTTTTCCGCCACCAACTCTGCTATCCAGCCCTCCGGCTGGGTGTGGGCAC‑CGCCTTCCATCAGGCTGCCGACCATATTTCTGACCatctg > 1:30066/1‑247 (MQ=255) gtGGCTACAGCCTGAAAGGTTGCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCTACCAGATACAGCCCTTCCTC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGt < 2:174331/250‑1 (MQ=255) tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGAATC‑TA‑CCGGACA‑GGGGAAACCACCGCGACCAGATAAAGCCCTTCCCC‑TTTTGCCCGTGCGGTCCCCAGCGTTCTGTCCTTTGTCGCCCGCAAATCTTCTTTCCCACGCTCCCGCTGGTTGTGGGGTA‑CGACTTTCCTCAGGCCGCCGAACATATTTCCGACCatcagatgt > 1:171424/1‑243 (MQ=255) caaCAGTCTGAAATGGTCGTAGCATTATTCTCGTTAGGCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGACA‑GGGGAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACGCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGGCAATATTCCTGACCAGATGATGTACaa < 2:290817/249‑1 (MQ=255) ccTGAAATGTTCCGGGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATG‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCAt < 2:373303/251‑1 (MQ=255) aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGA‑TCATA‑ACGGTCA‑GGGGAAACCACCCCGACCAGATACAGCCCTTCCGC‑TTTTTCCGTTTCTGCCGCCAGCGTTCTTTCCTTTTCCGCCCGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCCC‑CGACTTCCATCCGGCTGCCGCCAATATTCCTGACCATATGATGTACAAAAACATTCGc > 1:451228/1‑251 (MQ=255) aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑ACGGTCA‑GGGGAATCCACCCCGACCAGATACAGCCCCTCCCC‑TTTTTCCGTTTCTGCCCCCCGCGGTCTGTCCTTTTCCCCCAGCAACTCTGCTATCCCGCTCTCCCGCTGGGTGTGGGGAC‑CGGCTTCCCTCAGGCTGCCGCCAATATTCCTTACCATATGATGTACAAAAGCAATCGCtt > 1:138350/1‑251 (MQ=255) gctCCGAGCATTATCCTCGTTAGTCCGCCANAAACAGCGGGCCCATTTGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGCTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTCCCGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCTAGCGCTCCGGCTTGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTCCAAAAGCATTCGCttt < 2:253854/248‑1 (MQ=255) gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCttt < 1:242400/250‑1 (MQ=255) attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTgcc < 2:371459/113‑1 (MQ=255) ttCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGA‑TCATA‑CCGGTCA‑GGGGAATACACCCCGACCAGATACAACCCTTCCGC‑TTTTGCCGTTGCTTCCCCCCACGTTCTGTCCTTTGCCGCCAGCCACCCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCCC‑CGACTTCCCTCAGGCTGCCGACAATATTTCTGACCATATGATGTACAAAAACATTCGCTTTAAAATCTaccaccc > 1:477096/1‑250 (MQ=255) tcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGGAATCCACCGCGGCCAGATACAGCCCTTCCGC‑TTTTTTCGTTGCTGCCGCCAGCGGTCTTTCCTTTTTCGCCCGCAACTCTGCTCTCCACCTCTCCGGCTGGTTTTGGGGAC‑CGCCTTCCCTCCGGCTGCCGACAATATTTCTGACCATATGAAGTAAAAAAGCATTCGCTTTAATATCTACCACCACAt > 1:212509/1‑251 (MQ=255) cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGT‑TTTTGGAAGA‑TCATC‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCTTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAATCATTCGCGTTAATATCTACcaccac < 1:343492/248‑1 (MQ=255) aGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGa < 1:267627/251‑1 (MQ=255) gCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGGCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAc‑c < 2:162039/164‑1 (MQ=255) gAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGAcgc < 2:67258/251‑1 (MQ=255) tAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTa < 1:81116/224‑1 (MQ=255) cGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACAt < 1:242160/251‑1 (MQ=255) aTTGGAGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCCCCGCGACCAGATACATCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACGCTGCTATCCAGCTCTCCGGCTGGTTGTTGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATAGGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATg < 2:71742/251‑1 (MQ=255) ggCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTTCCACCACATAAGGACCGTGACGCGTGACATTAATATGCa < 2:373821/250‑1 (MQ=255) cGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATa > 1:263402/1‑250 (MQ=255) tttGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGt < 1:354427/250‑1 (MQ=255) ggAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTgcg < 2:210569/251‑1 (MQ=255) aTA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGATGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGAGCCACgggg > 1:216980/1‑251 (MQ=255) a‑CCGGTCA‑GGGTAATCCCCCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CTACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCTCATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGt < 1:474149/250‑1 (MQ=255) a‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGtt > 1:107239/1‑251 (MQ=255) cGGTCA‑GGGTAATCCACCGCGACCAGATACANCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCg < 2:239152/251‑1 (MQ=255) ggTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCCACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCt > 1:450907/1‑136 (MQ=255) ggTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCgg > 1:89037/1‑251 (MQ=255) ggTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTGGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCt < 2:450907/136‑1 (MQ=255) a‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGACt > 1:322862/1‑251 (MQ=255) | ACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TCATA‑CCGGTCA‑GGGTAATCCACCGCGACCAGATACAGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAC‑CGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGACT > NC_000913/2434656‑2435128 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |