| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 119,396 | 0 | C | T | 23.1% | 122.0 / 13.9 | 65 | S39F (TCT→TTT) | ampE | protein AmpE |
| Reads supporting (aligned to +/- strand): ref base C (13/37); new base T (15/0); total (28/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGC > NC_000913/119159‑119646 | tgCTATCCGGATATCGATAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAc < 2:446355/250‑1 (MQ=255) cgaTCCGGATATCGTTAAAAACATGACTGGCCATTGTGATATTGCGCCGGACCGGAAAACCGATCCCGGTCCTGCATTTGATTGGG‑ACGGGTTTCGTGTGCTGGTCAGCAATGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑Gtt < 2:360893/249‑1 (MQ=255) cTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACAATTTTTTCTCgggggccagtt > 1:38232/1‑243 (MQ=255) tCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACCTTTTTTTCTCgggggcccgggttg > 2:181895/1‑240 (MQ=255) ccGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑Gttcgg > 1:359864/1‑247 (MQ=255) atatCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTTTCTCCGGCGCCC‑GTTAGGC‑ATgg > 1:214594/1‑250 (MQ=255) atatCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAAATTTTTTTTTCGGGGCCAC‑GTTAgcatc > 2:483635/1‑246 (MQ=255) aCATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑GAACATTTTTTTCTCGGGCGCCC‑GGTTGGC‑ATGATCCTTATTGCgg > 1:4944/1‑250 (MQ=255) ccATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAAAATTTTTCTCTCGGGGGCAC‑GGTAAGC‑ATGACCATTATTGCGGTgggggggg > 1:190175/1‑245 (MQ=255) tgtgATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACttt < 2:116172/250‑1 (MQ=255) tgtgATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACttt < 1:466842/250‑1 (MQ=255) atatTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTAc < 1:448310/251‑1 (MQ=255) atTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACt < 1:65328/250‑1 (MQ=255) gtcgcCGGATCGGAAAACCGCTTCCGGTATTGCATTTGATTGGGCATTG‑TTGCGTGTGCTGGTCAGCACGGAGACTAC‑TGTCCGCTATGTACCACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCGTGAAGCTTGCTTTCGGCGGTTG‑AATCATGTTGCGCTCGGGCGCAC‑GTTAGGC‑TTGAGCATTATTGCGATGGGCTTG‑ACTTTTTTACTgc < 2:266467/248‑2 (MQ=255) cgcCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTCTCTCTGGCGCCC‑GTTAGGC‑ATGACCATTATTGCGAAGGGCGGG‑GCTTTTTTTCTGTTAc > 1:356249/1‑251 (MQ=255) aTCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑GAACATTTTTTTCTTCGGCGCCC‑GTTAGGC‑ATGACCCTTATTGCGATGGGCGGG‑GCTTTTTTTCTGTTACGCGCAt > 1:321675/1‑251 (MQ=255) acGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCAtt < 2:148948/250‑1 (MQ=255) gAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑GCTTTTTTTCTGTTTAGCGCATTGc > 1:135189/1‑250 (MQ=255) gAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCa < 2:190175/251‑1 (MQ=255) ccGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAg < 1:318673/251‑1 (MQ=255) gATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTTGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTa < 1:216983/251‑1 (MQ=255) caTCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACGGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTa < 2:483659/250‑1 (MQ=255) gTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTTTCCCGGGCGCAC‑GGTAAGC‑CAAACCCATATTTCGCTGGGGGGG‑GCTTTTTTATTGTTACGCGCCTTGCAggggatattttttc > 1:410052/1‑240 (MQ=255) cATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTTCGATGGGCGGG‑ACTTTTTTTTTTTTACGCGCCTTTCAGGGGGTATTGTTTAACGttt > 1:372537/1‑250 (MQ=255) tttGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGGTGGGCGGG‑GCTTTTTTTCTGTTACGCGCCTTGCAGGGGGTATTGTTCAACGtttcc > 1:295951/1‑248 (MQ=255) gATTGGGCACGG‑TTTCGTGTGCTGGTCAGCNAGGAGACAACATG‑ACGATATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCAc < 2:234734/250‑1 (MQ=255) tGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCt < 1:301376/250‑1 (MQ=255) gggCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACAATTTTCTCTCCGGCGCCCCGTT‑GGC‑ATGACCCTTATTGTGATGGGGGCG‑CCTTTTTTTCTGTTACGCTCCTTTCCGGGGGGTTTGTTCACCATTCCCACGCTAc > 2:129126/1‑251 (MQ=255) aCGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACCGGCAGCTTGATCATCGTCTTGAAGCGGTCTTTCGGCGGGTG‑AAACATTTTTCTCGCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGgt < 1:26561/251‑1 (MQ=255) cGTGTGCTGGTCAGCAAGGAGACCACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGgct < 2:18641/250‑1 (MQ=255) ggtgCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGgctgct < 1:262405/250‑1 (MQ=255) gtgCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGgctgct < 1:65203/250‑1 (MQ=255) gtgCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGgctgct < 1:198337/250‑1 (MQ=255) ggTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTgg < 2:295951/251‑1 (MQ=255) cAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTCTCTCGGGGGCCC‑GTTAGGC‑ATGACCCTTATTGCGGTGGGGGGG‑GCTTTTTTATTTTTACGCGCAGTGCAGGGGGGATTGTTTACCGTTCCCACCCTCCTGGGGGGGCTGCTGAtttgtttgtt > 2:389320/1‑249 (MQ=255) gagaCAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑GAACAATTTTCTCTCGGGCGCAC‑GGTAGGC‑CGGAACATTATTTCGGTGGGCGTGGATTTTTTTTCTGT‑ACGCGCCTTGCAGGGGGGATTGTTTTACGTTTCCACGATACTGgttgggggggtgatttgttttctgtgg > 1:182784/1‑226 (MQ=255) ttACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGt < 1:138718/250‑1 (MQ=255) tACATCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCAGCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATTGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGtt < 2:38232/250‑1 (MQ=255) gcAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑GCAAATTTTTCTCTCCCGCGCCA‑GTTAGGC‑ATGGCCATTATTTCGATGGGCGTG‑TCTTTTTTACTGTTTCGCGCCTTTCAGGGGGGATTTTTTCACGTTCCCCCCCTTCTGGTGTGGCTGCTGATTGGGTTGGTGTGTATTGGCGCAggggtagtttc > 2:265527/2‑242 (MQ=255) aCAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑GTGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTc < 1:392407/250‑1 (MQ=255) aCAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAAAATTTTTTTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTTCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCCTTGCAGGGGGTATTGTTCAAAGTTTCCACGCTACTGGGGTGGCTGCTGGTTGTTTTTCTgtgtttttgggccgggaaaatttt > 1:387116/1‑231 (MQ=255) cAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGAGTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGt < 1:181895/251‑1 (MQ=255) aaCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑GAACATTTTTTTCTCGGGCGCAC‑GTTAGGC‑ATGCCCATTATTGCGATGGGCGGG‑GCTTTTTTACTGTTACGCGCATTGCAGGGGGTATTGTTCAAACTTCCCACGCTACTGGGGGGGCTGCTGATTGGTTTGCTGTGTATTGGGGCAGGGAAAGttttgc > 1:32025/1‑247 (MQ=255) cTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAAAATTTTTCTTTTGGGCGCAC‑GCTAGGC‑ATTTCCATTATTTGGGTGGGCGTG‑ACTTTTTTACTGTTACGCGCATTTCAGGGAGGATTGTTCCACGTTCCCCCGCTACTGGTGTGGGTGGTGGTTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTTGTCTTc > 1:476437/1‑251 (MQ=255) tACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAAAATTTTTTTCTCGGGCGCAC‑GTTTGGC‑ATGACCCTTATTTCGATGGGGGTG‑ACTTTTTTTCTGTTACGCGCATTGCAGGGAGTATTGTTCTAAGTTCCCACGCTCCTGGTGGGGGTGCTGATTTGTTTGCTGTGTATTGGCGGCGGGAAAGTTCGTCTTcct > 2:170849/1‑249 (MQ=255) actcgtggtAATTTTCGAGCGCCTGTTTAAGTTGGGCGATCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTTGGCGTGTG‑AATCATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACTATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCAt < 2:478858/242‑1 (MQ=255) aCTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTTTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTTTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGGTTGGTTTGCTGTGTATTTGCGCAGGTAAAGTTCGTCTTCAtt > 1:374903/1‑251 (MQ=255) ggTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATc < 1:117420/251‑1 (MQ=255) gtgtTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCa < 1:147129/251‑1 (MQ=255) aaTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGGCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCGCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCtt < 2:939/251‑1 (MQ=255) cTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GGTAGGC‑ATGACCCTTATTTCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGGATTGTTCAACGTTTCCACGCTACTGGTGTGGCGTCTGATTGGTTTGCTGTGGATTGGCGCAGGTAAAGTTAGTCTTCATTATCATGCTTATCTGACAgctgc > 1:152587/1‑251 (MQ=255) gTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAgctgc < 1:247521/249‑1 (MQ=255) ttAAGTTGGGCGAGCACTGGCAGCTTTATCATCGTCTTGAATCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑TCTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTc < 1:90103/250‑1 (MQ=255) tAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCa < 1:366496/250‑1 (MQ=255) aCTGGCAGCTTGATCGTCGTCTTGAAGCGTGCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCAt < 2:4944/251‑1 (MQ=255) ggCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACCTTTTTTTTTCCGGCGCAC‑GCTAGGCCAGGACCAT‑CTTGCGATGGGGGTG‑GATTTTTTAATGGTACCCGCATTGCAGGGGGTGTTGTTCACCGTTCCCCCACTCCTGGTGTGGGCGCTGCTTGGTTTGCTGTGTTTTGGCGCCGGTAAAGGTCGGCTTCCTTTTCCTTCTTTTCTGGTGGCTGCTCCACGTAATGATAACCCTGcc > 2:160865/1‑251 (MQ=255) gCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGccc < 1:426042/251‑1 (MQ=255) aGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTTTCTCGGGGGCAC‑GTTTGGC‑TTGACCATTATTGAGATGTGCGTG‑GCTTTTTTACTGTTAAGCGCATTTCTGGGGGTATTGTTTACCGTTCCCCAGCTACTGGTGTGGCTGCTGTTTGGTTTGCTGTGTATTTGGGCCGTGAGAGTTTGTCTTCATTTTTATTCTTTTCTTACGGCCGCTTCACGTACAGATATCCATGCCCgc > 2:357903/1‑250 (MQ=255) aGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGt < 1:160865/251‑1 (MQ=255) tGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGAAGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGcc < 2:356249/250‑1 (MQ=255) cTTGAAGCGTTCTTTCGGCGGGAG‑AAACATTTTTTTCTCGGGGGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTAAGCGCCTTTCAGGGAGTATTGTTCAACGTTCCCACGCTCCTGGTGTGGCTGCTGATTGGTTTTCTGTGTATTGGCGCAGGGAAAGTTCGGCTTCATTATCATGCTTATCTGAAAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACCAtggctgg > 1:90751/1‑251 (MQ=255) aGCGTTCTTTCGGCGGGTG‑AAACATTTTTTTCTCCGGCGCAC‑GGTAGGC‑ATCACCATTCTTTCGATGGGCGTG‑ACTTTTTTTCTGTTACGCGCCTTGCAGGGAGGATTGTTAAACGTTCCCACGCTACTGGGGTGGGTGCTGATTGGTTTTGTGTGTATTTGCGCAGGTCAAGGTCGTCTTCATTATCATGCTTTTTTGACAGCTGCTTCACGTAATGANAACCATGCCCGTGCCACGAtggcggggggac > 2:233840/1‑243 (MQ=255) gTTCTTTCGGCGGGGG‑ACACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGGGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTc > 1:349522/1‑250 (MQ=255) ttCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCAt < 2:135189/250‑1 (MQ=255) aCATTTTTCTCTCTGGCTCAC‑GTTAGTC‑CTGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGGTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTcc < 2:72217/251‑1 (MQ=255) tctctcGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAg < 1:397964/250‑1 (MQ=255) ctctcGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGc > 1:365836/1‑251 (MQ=255) | TGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGG‑TTTCGTGTGCTGGTCAGCAAGGAGACAACATG‑ACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTCGGGCGCAC‑GTTAGGC‑ATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGC > NC_000913/119159‑119646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |