Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,813,272	0	C	T	21.3%	105.9 / 12.4	61	S181L (TCG→TTG)	dfp	fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase
Reads supporting (aligned to +/- strand):  ref base C (4/44);  new base T (13/0);  total (17/44)
Fisher's exact test for biased strand distribution p-value = 3.62e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGC  >  NC_000913/3813032‑3813518                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                gAATGGCGAATTCCCTGGTATCGACGTTTTGTCTGGCTCCCCCTGCCCCTGTACCCGTGCCCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAAc                                                                                                                                                                                                                                                       <  1:107186/251‑1 (MQ=255)             ccctggtaccgtagaTTTGTCTGGCTACCCCGGCCCCTGTAGCCTTGCTCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACAt                                                                                                                                                                                                                                         <  1:365002/238‑1 (MQ=255)               cTGGTATCGACGATTTGTCTGGCTCCAACTGCGCCTGTAGCCGTGCTCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGGGATATCGGTCCTGGGCGAATGCGAGATCCGTTACCCATTGTGGATATGGCGGTGAC‑GC‑ACTTTTTGCCCGGCAAcaccacttaaacaac                                                                                                                                                                                                                                         >  2:344977/1‑237 (MQ=255)               cTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TCTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGG‑GC‑CTTTTTTGccccgccaccaccctaaacacct                                                                                                                                                                                                                                         >  2:333754/1‑231 (MQ=255)                  gTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGG‑GCCATTTTTTCCCCG‑CAACG‑ACCtcacaaatctga                                                                                                                                                                                                                                      >  1:172324/1‑239 (MQ=255)                    aacggcgaTTTGTCTGGCTACCCCGGCGCCGGTGCCCGGCCCCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑c                                                                                                                                                                                                                                  <  2:490598/245‑1 (MQ=255)                     ccgttgtttgtcctgtccaccccggcgcCTGTAGCCTGGCTCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CAt                                                                                                                                                                                                                                <  2:475287/227‑1 (MQ=255)                      cggcgttttGTCTGGCTACCCCTGCGCCTGTGGCCGGGCCCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CAt                                                                                                                                                                                                                                <  1:162117/244‑1 (MQ=255)                           ttttGTCGGGCTACCCCTGCCCCTGTGGCCTTCCTCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATg                                                                                                                                                                                                                            <  1:479330/248‑1 (MQ=255)                               gTCTGGCTCCCCCTGCGCCTGTAGCCGTGCCCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTAc                                                                                                                                                                                                                       <  1:23982/250‑1 (MQ=255)                                   ggCTACACCTGCGCCTGTAGCCGTGCTCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGA‑GC‑CTTTTTTGCCCGCCAACG‑ACCCGA‑AACATCTTAA‑CATTTTTATTTccgccc                                                                                                                                                                                                                  >  1:314746/1‑250 (MQ=255)                                      cacccccgcgccggggccggtgtcccccGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCGGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGcc                                                                                                                                                                                                               <  2:277122/227‑1 (MQ=255)                                                     gccgggctcccccGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAAGTTAGAGGTGCTGGTTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑CCCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGc                                                                                                                                                                                                <  1:28870/242‑1 (MQ=255)                                                        cgtcccccccGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCGGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCg                                                                                                                                                                                             <  1:217691/245‑1 (MQ=255)                                                        cggcccccccGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCg                                                                                                                                                                                             <  1:394016/245‑1 (MQ=255)                                                           cccccccGCCCATGACCCGGCAGATGTACCGTGCCGCGGCCCCGCAGCATAATTTAGAGGTGCTTGCTTCCCTTGG‑TTTGCGCATCTGGGGGCCAGACAGGGG‑CAGTCAGGCTTGTGGTGATATCGTTCCTGGGGTAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑GCCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑At                                                                                                                                                                                          <  2:205352/248‑1 (MQ=255)                                                              ccccggccatgccccggcagATGTACCGGGCNGCTGCCACGAAGCATAATTTAGTGGTGTTTGCTCCCCGTGG‑TTGGCTCATCTGGGGGCCAGACTGTGG‑CAGTTAGGCTGGTGGTGATAGCGGTCCGGGGCGAATGCTGGGTCCGTTAACCATTGGGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑CCCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑g                                                                                                                                                                                      <  2:242104/235‑1 (MQ=255)                                                                       tGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑CTTTTTCTCCCGGCCACG‑ACCTGA‑AAAATATGTA‑CATTATGATTACCCCCCGCCCCACGCCGGGACCGCTCC‑ATCC‑GGGGCGtttt                                                                                                                                                                             >  1:48540/1‑249 (MQ=255)                                                                         aaacggcagATGGACGGTGCCTCGGCCACGCAGCATAATTTAGAGGTGGCTGCTTCC‑GTGGGTTTCCTCACGTGGGGCCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATACCGGTCCTGGGCGATTGCCCGATCCGTCAACCCTTGTGGATATGGGAGTAGC‑CC‑ATTTTTCGCCCGTCAACG‑CCCGGA‑CACATCTGAA‑CATTCTGATTACCGCCGGCCCGCCGCGGGAACCGCTCG‑ATCC‑GGTGCGTTatat                                                                                                                                                                           <  2:448673/246‑1 (MQ=255)                                                                            cagcagAGGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATAtctc                                                                                                                                                                        <  1:133512/251‑1 (MQ=255)                                                                                   attgcctggccgctacctgccagctTAATTTTGTGGTGCTTGCTTCCCGTGG‑TTTCACCATCTGGGGGCCCGGCCGTGC‑CCTTCACGCTTGTGATGCCAACGGTCCTGGGGTACTACTCGATCCGTTAACCATGGTGGATATGGCTGTGCC‑GA‑ATTTTCCGCCTATCAACG‑CCCTAA‑ACCAGCAGCA‑CATTATGATTCCCGCCGGCCCCACGCGTGACCCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATcac                                                                                                                                                                 <  1:462537/231‑1 (MQ=255)                                                                                      cccGTGCCGCTGCCCCGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGc                                                                                                                                                              <  1:211171/250‑1 (MQ=255)                                                                                            ccGCGGCCCCGCAGCATAATTTAGAGTTGCTTGCGTCCCGTGG‑TTGGCTCATCTGGGGGCCAGACAGTGG‑CAGGCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGTTGGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGc                                                                                                                                                        <  2:314746/251‑1 (MQ=255)                                                                                                 gCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGT‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAg                                                                                                                                                     <  1:454687/249‑1 (MQ=255)                                                                                                         gcaTAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCg                                                                                                                                                                                 <  1:101983/213‑1 (MQ=255)                                                                                                              attaagagGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGTTCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATc                                                                                                                                      <  2:104471/247‑1 (MQ=255)                                                                                                                          ttgcttCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATcc                                                                                                                                                                                        <  1:139390/190‑1 (MQ=255)                                                                                                                           tgcttCCCTTGG‑TTGGCTCATCTGGGGGCCAGAC‑GTGGGCGGTCAGGTTTGTGGTGATATCGGTCTTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑TTTTTTCGCCGGTCAACG‑ACCTAA‑AACATCTAAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCt                                                                                                                          <  1:477893/250‑1 (MQ=255)                                                                                                                            gcttCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGG‑GC‑CTTTTTTTCCCGTCAACG‑ACCCGA‑AAAATCTGAA‑CATTATTATTTCCCCCCGCCCCACCCCTGAACCGCTCG‑ATCC‑CGGGCGTTTTTTCTCTAAACACAGCTCCGGCCAGAAGGGgttttcttttcccgcccccccttc                                                                                                                        >  1:93759/1‑231 (MQ=255)                                                                                                                               tCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGGGG‑CAGTCAGGCTTGGGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑TTTTTTCGCCCGTCAACG‑CCCTGA‑AACATCTGAA‑CATTATGATTTCCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGccc                                                                                                                      <  1:461035/250‑1 (MQ=255)                                                                                                                                   gTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑CTTTTTTGCCCGTCAACG‑ACCTGA‑AACATCCTAA‑CAATATTATTACCCCCGGCCCCACCCCTGAACCCCCCC‑ACCC‑CGTGCGTTTTATCTCTAATCACAACTCCGGCCAAATGGGGTTTTCTATcgcccccgcccccgcccctcgg                                                                                                                 >  1:469264/1‑239 (MQ=255)                                                                                                                                    ggggttgccTCATCTGGGGGCCAGCCGGTGG‑CGTTCAGGTTTGTGGTGATATGGGTCCTGGGCGAATGCTCGTTCCGTTAACCATTGGGGATATGGCGGTAGT‑GC‑ATTTTTCGCCCGTCAACG‑CCCTGA‑AACATCTGAA‑CATTATGATTCCCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCcgtcgt                                                                                                                 <  2:94256/243‑1 (MQ=255)                                                                                                                                      g‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGG‑GC‑CTTTTTTTCCCGTCCAAG‑ACCCTA‑GACATCTGAAACAT‑ATGATTTCCCCCCGCCCGCCGCGTGAATCGCCCG‑CTCCCGGT‑CGGTATTTTTCTAATCACCGCTCCCGGAAGATGGGGTTTTCTCTccccgcccccccgccccgtccgggg                                                                                                              >  2:199776/1‑233 (MQ=255)                                                                                                                                                   gggggctggacgtggg‑CAGTCAGGTTTGGGGTGATGTCGGCCCTGGGCGATTGCTCGTTCCGTTACCCATTGTGGATATGGCGGTCGC‑GC‑ATTGTTCGCCCGTCAACG‑ACCTAA‑ACCATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAc                                                                                                   <  2:498986/236‑1 (MQ=255)                                                                                                                                                          ggccctcccgatttCAGGCTTGTGGTGTTACCTGTCCGGGGGGAATGCTCGATCCGTTATCCATGGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCTCCAACG‑ACCAGA‑AACATCTGAA‑CATTAGGATTACCGCCGGCCCGACGCGTGAACCGCGCG‑AGCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTa                                                                                          <  1:214249/238‑1 (MQ=255)                                                                                                                                                               gggg‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGt                                                                                    <  1:186661/249‑1 (MQ=255)                                                                                                                                                                        cAGGCTTGTGGTGGTATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATGGTGGATATGGCGGTAGC‑GC‑TTTTTTCGCCCGTAAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGttt                                                                             <  1:200733/250‑1 (MQ=255)                                                                                                                                                                         aGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCTTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTCCCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCa                                                                           <  1:327898/251‑1 (MQ=255)                                                                                                                                                                                       tccGGTCCTGGGAGAATGCCGGACCCTTTAACCATTTTGGATAGGGGTGTGGC‑GC‑TTTTTTCGCCCTCCAACG‑ACCGGA‑AACATCTGAC‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCAc                                                             <  2:399300/248‑1 (MQ=255)                                                                                                                                                                                       atCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTTCCCCCCCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCCGCCCGACGCGTGGACCGCCCG‑ATCC‑GGTGCGTTATATTTCTAATCACAACCCCGGCAAGATGGGTTTTGCTATCGCCGCCCCCGCTGCCCGTCGTGGGGCGGACGGCAC‑GC‑CGGGATCAGGTCCGGGTTTACTACCGAC‑GCCAcc                                                            >  1:448859/1‑251 (MQ=255)                                                                                                                                                                                        ccgttCCTGGGCGAATGCTCAATCCGTTACCCATGGTGGATATGTTGGTAGC‑CC‑ATTTTTCGCCCTTCAACG‑ACCTGA‑AACATCTAAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCTAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCg                                                           <  1:378021/247‑1 (MQ=255)                                                                                                                                                                                             ccTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTTGCCCGTCCACGCACC‑GC‑AACATCTTAA‑CCTTATGACTACCGCCGGCCCGACCCGGGAACCACTCG‑ATCC‑CGGGCGTTATATCTCCAATCACAACCCCGGCAAGAAGGGTTTTTCTATTGCCCCCCCCGCCCCCCCTCGTGGCGCGAACGTCAC‑GC‑CGGGATCAGGGCCGGTTTCACTACCGAC‑GCCCCCG‑ttttt                                                     >  1:427410/1‑249 (MQ=255)                                                                                                                                                                                              cggggCGAATGCCCGATCCTGTAACCATTGGNGATATGGCGG‑AGCCGA‑ATTTTCCGCCCGCCACCG‑ACCTGA‑ACCATCTGAA‑CTTTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGtt                                                    <  2:232682/249‑1 (MQ=255)                                                                                                                                                                                                ccgaatcggcgcGATCCGTTAACCTTTGTGGTTAGGGGGGTAGC‑GT‑TTTTTTCGCCCGCCAACG‑ACCTGA‑AACAGCTGAC‑CTTTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTTTCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTa                                                   <  2:316621/239‑1 (MQ=255)                                                                                                                                                                                                 ggCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑CTTTTTTGCCCGTCAAAG‑ACCCGA‑AACATCTGAA‑CATTATGATTACCCCCGGCCCGCCGCGTGAACCGCTCG‑ATCC‑CGGGCGTTATATCTCTAATCACAACCCCGGCAAGATGGGGTTTGCTATTCCCGCCCCCGCTGCCCGCCGTGGCGCGGACGCCAC‑GC‑TGGGATCCGGGCCGGGTTCACTAcccccgcccccgcttttttt                                                    >  2:101900/1‑232 (MQ=255)                                                                                                                                                                                                    gAATGCTCGATCCGTTAACCATTGGGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑CCCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACgt                                              <  1:394313/251‑1 (MQ=255)                                                                                                                                                                                                       tGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTTGCCCCTCCACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCCACCCGTGAACCCCCCG‑ATCC‑CGTGCGGTATATCCCTAATCACAACTCCCGCAAGATGGGGTTTTCTATCGCCGCCGCCGCTGCCCGTCCTGGCGCGAACGGCAC‑GC‑TGGGATCAGGGCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGtt                                           >  1:186825/1‑251 (MQ=255)                                                                                                                                                                                                         cTCGATCCGTTAACCATTGTGGATATGGCGGTAAC‑GC‑ATTTTTTGCCCGTCAACA‑ACCTGA‑AAAATCTTAA‑CATTATTATTACCGCCGGCCCGACGCGTGAACCGCACGCATC‑‑GGGGCGGTATATATCTAATCAAAACTCCGGCAAGATGGGGTTTGCTATCCCCGCCCCCGCTGCCCGTCGTGGCGCGCACGTCAC‑GC‑TGGGTTCAGGGCCGGGTTTACTACCCAC‑GCCACCG‑TTTGTTAAAAGTGTtgg                                         >  1:478510/1‑250 (MQ=255)                                                                                                                                                                                                            gATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGTtgatgt                                      <  1:88912/251‑1 (MQ=255)                                                                                                                                                                                                             acccgtaaccctttggggatgtgtcGGTAGC‑GC‑ATTTGTCGCCCGCCCACC‑ACC‑GAGAACATCTAAA‑CATGATGATTACCGCCGGCCCGACGCGTGAACCGCGCG‑ATCC‑TGTGCGTTATATCTCTATTCACAGCTCCGGCATGAGGGGTTTTGCTCTCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCTC‑GC‑TGGTATCAGGTCCTGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGTtgatgtg                                     <  2:132577/227‑1 (MQ=255)                                                                                                                                                                                                             aatttttAACCTTTGGGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGCCAACG‑CCCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAGGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGTtgatgtg                                     <  2:75301/246‑1 (MQ=255)                                                                                                                                                                                                                  ttAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTTGCCCGTCAACC‑ACCTGA‑AACAACCTAA‑CAATATGATTACCGCCGGCCCGACCCGGGGACCGCCCG‑AACC‑GGGGCGGTATATCTCTTATCACCGCCCCGGCAAAATGGGGTTTGCTATCCCCGCCCCCCCCGCCCCTCGTGGCGCGGACCGCAC‑CCCTGG‑ATCAGGGCCGGGTTCACTACCCAC‑GCCCCCGGTTTGTTaaagaggtggtgtgggatc                                 >  1:75301/1‑235 (MQ=255)                                                                                                                                                                                                                    aaCCATTGTGGATAGGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAC‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGtt                                           <  1:263258/238‑1 (MQ=255)                                                                                                                                                                                                                             ggaaatGGCGGTAGC‑GA‑ATTGTTCGCCCGTCAACT‑CCCTGA‑AACATCTGAA‑CTTTATGTTTCCCGCCGGCACGACGCGTGAACCTCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGTTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGTTGATGTGATGACCGCGCTGGaaa                     <  1:494402/247‑1 (MQ=255)                                                                                                                                                                                                                                 atGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGa                 <  1:71930/251‑1 (MQ=255)                                                                                                                                                                                                                                 atGGCGGAAGC‑CC‑ATCTTTCGCCCGCCAACG‑CCCTGA‑AACATCTGGA‑AAATAAGATTTCCAACGGTCCGGAGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCCCAGCTACGGCAAGCTGGACTTTGCTCTCGCCGCCGCCGCGACCCGTACTGGCGCGGACGT‑ACTGC‑TGGGAGCAGGTCCCGGTTCGCTAACGACCGCC‑CCA‑GTTGCTATACGCGTTGATGTGATGGACACGCTGGAAATgg                  >  2:286346/1‑250 (MQ=255)                                                                                                                                                                                                                                  gggCGGTAGC‑GC‑ATTTTCCGCCCCGCAACG‑ACCTGA‑AACATCGGAC‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGaa                <  2:176193/250‑1 (MQ=255)                                                                                                                                                                                                                                       gTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTAA‑AACATCTGAA‑AATTAGGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAgccgc           <  1:235972/251‑1 (MQ=255)                                                                                                                                                                                                                                         aGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAgccgcc          <  1:318706/250‑1 (MQ=255)                                                                                                                                                                                                                                         aGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGccgccg         >  1:418436/1‑251 (MQ=255)                                                                                                                                                                                                                                          gc‑gc‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGGGTTGATGTGATGACCGCGCTGGAAATGGAAGccgccgg        >  1:471884/1‑250 (MQ=255)                                                                                                                                                                                                                                                   ttccGCCCGTCAACG‑CCCTAA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGc  <  1:333754/247‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                GAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCC‑ATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGG‑TTTGCTCATCTGGGGGCCAGACAGTGG‑CAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGC‑GC‑ATTTTTCGCCCGTCAACG‑ACCTGA‑AACATCTGAA‑CATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCG‑ATCC‑GGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAC‑GC‑TGGTATCAGGTCCGGTTTCACTACCGAC‑GCCACCG‑TTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGC  >  NC_000913/3813032‑3813518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑