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breseq version 0.39.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
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evidence | position | mutation | annotation | gene | description |
RA | 155,463 | A→T | *247K (TAA→AAA) | yadV ← | putative fimbrial chaperone YadV |
MC JC | 257,908 | Δ776 bp | insB9–[crl] | insB9, insA9, [crl] | |
RA | 557,635 | A→T | L36Q (CTG→CAG) | folD ← | bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase |
MC JC | 575,786 | Δ991 bp | IS5‑mediated | nmpC ← | putative outer membrane porin NmpC |
JC JC | 577,688 | IS3 (+) +3 bp | coding (76‑78/498 nt) | rrrD → | lysozyme |
RA | 1,048,596 | G→A | G32G (GGC→GGT) | gfcC ← | capsule biosynthesis GfcC family protein |
MC JC | 1,048,805 | Δ48,085 bp | [gfcB]–ycdU | 45 genes [gfcB], gfcA, insA4, insB4, cspH, cspG, ymcF, ymcE, gnsA, yccM, torS, torT, torR, torC, torA, torD, cbpM, cbpA, yccE, agp, yccJ, wrbA, ymdF, rutG, rutF, rutE, rutD, rutC, rutB, rutA, rutR, putA, ymdG, putP, efeO, efeB, phoH, pgaD, pgaC, pgaB, pgaA, dgcT, insF4, insE4, ycdU |
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JC JC | 1,105,376 | IS2 (–) +5 bp | intergenic (+83/‑34) | csgC → / → ymdA | curlin chaperone/uncharacterized protein YmdA |
RA | 1,196,861 | A→G | intergenic (+79/+6) | C0293 → / ← ymfD | small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD |
MC JC | 1,196,867 | Δ27,041 bp | [ymfD]–ymgJ | 37 genes [ymfD], ymfE, lit, intE, xisE, ymfH, ymfI, ymfJ, ymfK, ymfT, ymfL, ymfM, ymfN, ymfR, beeE, ymfQ, ycfK, tfaP, tfaE, pinE, mcrA, iraM, ymgK, ymgL, ycgX, bluR, bluF, ycgZ, ymgA, ariR, ymgC, pdeG, ymgF, ymgD, ymgG, ymgI, ymgJ |
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RA | 1,301,917 | C→T | Q246Y (CAG→TAT) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
RA | 1,301,919 | G→T | Q246Y (CAG→TAT) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
RA | 1,337,394 | A→G | S522G (AGC→GGC) | acnA → | aconitate hydratase 1 |
RA | 1,652,331 | T→C | intergenic (+2346/+397) | ydfD → / ← ynfP | lysis protein/protein YnfP |
JC JC | 2,016,437 | Δ2 bp :: IS186 (–) +8 bp :: Δ1 bp | coding (570‑577/687 nt) | fliH → | flagellar biosynthesis protein FliH |
RA | 2,032,423 | G→A | V219V (GTC→GTT) | yedJ ← | putative HD superfamily phosphohydrolase YedJ |
RA | 2,867,454 | 2 bp→AA | coding (97‑98/993 nt) | rpoS ← | RNA polymerase sigma factor RpoS |
MC JC | 3,110,591 | Δ24,234 bp | [yghD]–yghT | 20 genes [yghD], yghE, yqhJ, yghF, yghG, pppA, yghJ, glcA, glcB, glcG, glcF, glcE, glcD, glcC, yghO, insH9, yghQ, yghR, yghS, yghT |
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RA | 3,486,205 | C→A | T29K (ACG→AAG) | crp → | DNA‑binding transcriptional dual regulator CRP |
RA | 3,560,455 | +G | intergenic (+589/+167) | rtcR → / ← glpG | DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG |
JC JC | 3,707,484 | +GA :: IS3 (+) +4 bp | coding (219‑222/1608 nt) | dppA ← | dipeptide ABC transporter periplasmic binding protein |
JC JC | 3,720,136 | IS3 (–) +3 bp | coding (88‑90/213 nt) | cspA → | cold shock protein CspA |
JC JC | 3,890,411 | IS1 (–) +9 bp | coding (176‑184/1248 nt) | tnaB → | tryptophan:H(+) symporter TnaB |
RA | 4,235,651 | T→C | intergenic (+244/‑255) | pgi → / → yjbE | glucose‑6‑phosphate isomerase/uncharacterized protein YjbE |
MC JC | 4,235,905 | 16376 bp→52 bp | yjbE–malM | yjbE, yjbF, yjbG, yjbH, yjbT, psiE, xylE, malH, malG, malF, malE, malK, lamB, malM | |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
MC JC | 4,299,522 | Δ12,535 bp | mdtP–alsB | mdtP, mdtO, mdtN, ytcA, yjcS, alsK, alsE, alsC, alsA, alsB | |
RA | 4,312,070 | Δ1 bp | intergenic (‑28/+31) | alsB ← / ← alsR | D‑allose ABC transporter periplasmic binding protein/DNA‑binding transcriptional repressor AlsR |
RA | 4,312,151 | G→C | Q281E (CAA→GAA) | alsR ← | DNA‑binding transcriptional repressor AlsR |
RA | 4,312,215 | A→G | T259T (ACT→ACC) | alsR ← | DNA‑binding transcriptional repressor AlsR |
RA | 4,312,219 | A→G | L258P (CTA→CCA) | alsR ← | DNA‑binding transcriptional repressor AlsR |
RA | 4,312,289 | A→G | Y235H (TAT→CAT) | alsR ← | DNA‑binding transcriptional repressor AlsR |
RA | 4,312,498 | C→G | R165P (CGC→CCC) | alsR ← | DNA‑binding transcriptional repressor AlsR |
RA | 4,313,027 | T→G | intergenic (‑36/‑323) | alsR ← / → rpiB | DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B |
MC JC | 4,313,864 | Δ16,405 bp | yjdP–rdcB | 18 genes yjdP, phnP, phnO, phnN, phnM, phnL, phnK, phnJ, phnI, phnH, phnG, phnF, phnD, phnC, yjdN, yjdM, rdcA, rdcB |
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RA | 4,337,446 | C→A | D162Y (GAC→TAC) | adiY ← | DNA‑binding transcriptional activator AdiY |
RA | 4,558,907 | T→A | K207M (AAG→ATG) | iadA ← | isoaspartyl dipeptidase |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1978495 | 1979270–1978507 | 13–776 | 20 [0] | [1] 17 | insB5–insA5 | insB5,insA5 |
* | * | ÷ | NC_000913 | 2406788 | 2407179 | 392 | 44 [0] | [0] 38 | lrhA/alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA |
* | * | ÷ | NC_000913 | 3423871–3424231 | 3424426–3424238 | 8–556 | 6 [4] | [4] 5 | [rrlD] | [rrlD] |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 21 (0.630) | 21/478 | 0.6 | 100% | intergenic (+253/‑69) | ychE/insH21 | MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+151/‑484) | insH21/oppA | IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | = 1397238 | NA (NA) | 20 (0.590) | 18/486 | 0.8 | 100% | noncoding (1195/1195 nt) | IS5 | repeat region |
? | NC_000913 | = 1978494 | 0 (0.000) | intergenic (‑297/+24) | flhD/insB5 | DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB | |||||
* | ? | NC_000913 | 1978503 = | NA (NA) | 44 (1.310) | 38/484 | 0.1 | 100% | noncoding (768/768 nt) | IS1 | repeat region |
? | NC_000913 | = 2406787 | 0 (0.000) | intergenic (‑146/‑774) | lrhA/alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA | |||||
* | ? | NC_000913 | 1979271 = | 1 (0.030) | 16 (0.470) | 14/486 | 1.1 | 94.1% | intergenic (‑56/‑482) | insA5/uspC | IS1 family protein InsA/universal stress protein C |
? | NC_000913 | = 2102947 | NA (NA) | pseudogene (443/446 nt) | wbbL | interrupted rhamnosyltransferase WbbL | |||||
* | ? | NC_000913 | 2407180 = | 0 (0.000) | 38 (1.130) | 35/484 | 0.1 | 100% | intergenic (‑539/‑381) | lrhA/alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA |
? | NC_000913 | 3583428 = | NA (NA) | noncoding (1/768 nt) | IS1 | repeat region |