breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 155,463 A→T *247K (TAA→AAA)  yadV ← putative fimbrial chaperone YadV
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
MC JC 575,786 Δ991 bp IS5‑mediated nmpC ← putative outer membrane porin NmpC
JC JC 577,688 IS3 (+) +3 bp coding (76‑78/498 nt) rrrD → lysozyme
RA 1,048,596 G→A G32G (GGC→GGT gfcC ← capsule biosynthesis GfcC family protein
MC JC 1,048,805 Δ48,085 bp [gfcB]ycdU 45 genes
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,196,861 A→G intergenic (+79/+6) C0293 → / ← ymfD small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD
MC JC 1,196,867 Δ27,041 bp [ymfD]ymgJ 37 genes
RA 1,301,917 C→T Q246Y (CAG→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,301,919 G→T Q246Y (CAG→TAT oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
JC JC 2,016,437 Δ2 bp :: IS186 (–) +8 bp :: Δ1 bp coding (570‑577/687 nt) fliH → flagellar biosynthesis protein FliH
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,867,454 2 bp→AA coding (97‑98/993 nt) rpoS ← RNA polymerase sigma factor RpoS
MC JC 3,110,591 Δ24,234 bp [yghD]yghT 20 genes
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
JC JC 3,707,484 +GA :: IS3 (+) +4 bp coding (219‑222/1608 nt) dppA ← dipeptide ABC transporter periplasmic binding protein
JC JC 3,720,136 IS3 (–) +3 bp coding (88‑90/213 nt) cspA → cold shock protein CspA
JC JC 3,890,411 IS1 (–) +9 bp coding (176‑184/1248 nt) tnaB → tryptophan:H(+) symporter TnaB
RA 4,235,651 T→C intergenic (+244/‑255) pgi → / → yjbE glucose‑6‑phosphate isomerase/uncharacterized protein YjbE
MC JC 4,235,905 16376 bp→52 bp yjbEmalM yjbE, yjbF, yjbG, yjbH, yjbT, psiE, xylE, malH, malG, malF, malE, malK, lamB, malM
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
MC JC 4,299,522 Δ12,535 bp mdtPalsB mdtP, mdtO, mdtN, ytcA, yjcS, alsK, alsE, alsC, alsA, alsB
RA 4,312,070 Δ1 bp intergenic (‑28/+31) alsB ← / ← alsR D‑allose ABC transporter periplasmic binding protein/DNA‑binding transcriptional repressor AlsR
RA 4,312,151 G→C Q281E (CAA→GAA)  alsR ← DNA‑binding transcriptional repressor AlsR
RA 4,312,215 A→G T259T (ACT→ACC alsR ← DNA‑binding transcriptional repressor AlsR
RA 4,312,219 A→G L258P (CTA→CCA)  alsR ← DNA‑binding transcriptional repressor AlsR
RA 4,312,289 A→G Y235H (TAT→CAT)  alsR ← DNA‑binding transcriptional repressor AlsR
RA 4,312,498 C→G R165P (CGC→CCC)  alsR ← DNA‑binding transcriptional repressor AlsR
RA 4,313,027 T→G intergenic (‑36/‑323) alsR ← / → rpiB DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B
MC JC 4,313,864 Δ16,405 bp yjdPrdcB 18 genes
RA 4,337,446 C→A D162Y (GAC→TAC)  adiY ← DNA‑binding transcriptional activator AdiY
RA 4,558,907 T→A K207M (AAG→ATG)  iadA ← isoaspartyl dipeptidase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978495 1979270–1978507 13–776 20 [0] [1] 17 insB5–insA5 insB5,insA5
* * ÷ NC_000913 2406788 2407179 392 44 [0] [0] 38 lrhA/alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
* * ÷ NC_000913 3423871–3424231 3424426–3424238 8–556 6 [4] [4] 5 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)21 (0.630) 21/478 0.6 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)20 (0.590) 18/486 0.8 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1978503 =NA (NA)44 (1.310) 38/484 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 2406787 0 (0.000)intergenic (‑146/‑774) lrhA/alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
* ? NC_000913 1979271 =1 (0.030)16 (0.470) 14/486 1.1 94.1% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 2407180 =0 (0.000)38 (1.130) 35/484 0.1 100% intergenic (‑539/‑381) lrhA/alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
?NC_000913 3583428 = NA (NA)noncoding (1/768 nt) IS1 repeat region