Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	56.9 / NA	20	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/11);  total (9/11)

GCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCAC  >  NC_000913/1337166‑1337638                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     gCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggtgcggt                                                                                                                                                                                                                                <  1:276291/251‑1 (MQ=255)       ggCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCTCCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTATCCTTCGGTGCGGTGCTGTc                                                                                                                                                                                                                          <  1:263058/251‑1 (MQ=255)          ttGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCACGCGGCAACCTTGGGTCAAAGCGTCGCTGGAAACGGGTTCGAAAGTCGTGTCTGATTATCTGGCAACAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCTCCTGTCTTGGTAACTCTATGCCGCTGCCCGATCCTATCGAAAAGGCAATCACAAAAGGCGATTTAACCGTCGGTGCGGCGCTGtatgg                                                                                                                                                                                                                       >  2:241748/1‑247 (MQ=255)           tGCTGGCGAAAAAAGCCGTAACTCTGGGCCTAAAGCGGCAACCCTGGGTCAAAGCGTAGCTGGAACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACAACGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATAAGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAAGGGCCATCAAAAAAGGCGATTTACACGTCGGTGCGGTGCTGTCCGGc                                                                                                                                                                                                                      >  2:27882/1‑251 (MQ=255)               ggCGAAAAAAGCCGTAGCTCTGCGCCTCAAGCGTCAACCATGGGTCAAAGCGTCGCTGGCACCGGGGTGGAAAGTCGTTTCTGATTATCTGGCGAAAGCGAAACTGTCTCCGTATCGCGACGAACTGGGGTTTATCCTTGTGGGATACGCTTGTGCCCTCTTTATTGGTAGCGCTGGGCCGCTGTGCGATCCTATCGAAACCGCAAGCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCa                                                                                                                                                                                                                     <  1:235702/248‑1 (MQ=255)                                          cAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccatcc                                                                                                                                                                                         <  1:74181/249‑1 (MQ=255)                                            aGCGGCCACCATGGGTCAAAGCGTCGCTGGCAACGGGTTCGAAAGTCGTATCTGGTTATCCTGCAAAGGCGAAACTGCCACCGTCTCTCGACGGACTGGGGTTTAACCTTGGGGGATTCGGTTGTACCCCCTGTATTGGTAACTCTGGGCCGCTGCCCGAACCGCTCGAAACGGCAATCAAAAAAGGCGATTTAGCCGTGGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGTTGTATCCATCCGCTg                                                                                                                                                                                     <  2:128601/251‑1 (MQ=255)                                                           tCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActgg                                                                                                                                                                      >  1:272334/1‑251 (MQ=255)                                                                                                          gCAAAAGCGAAACTGACACCGTATCTCTACGATCTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTTCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGTCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAatat                                                                                                                       <  1:114149/251‑1 (MQ=255)                                                                                                                    aaCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAAc                                                                                                             <  1:198897/251‑1 (MQ=255)                                                                                                                              gTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGGGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTg                                                                                                   >  1:52821/1‑251 (MQ=255)                                                                                                                                     gacgaaatgtgttatacccTTGTGGGTTACGGTTGTACCACCTGAATTGGTAACTCTGGGCGGCTGCCCGATCCTATCGAAACCGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGTTGCTGTCCGGCAACCGTAACTTTGAAGTCCGTATCCATCCTCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTTGTTGCCTATGCGCTGGCGGTAAATATGAATCTCAACCGGGCTTCTGAGCCTAt                                                                                            <  2:32577/234‑1 (MQ=255)                                                                                                                                                                                            ctGGGCCGCTGCCCGATCCTATCGAACCGGCAATCAAAAAAGGCGCTTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGAAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGACTATCAACCTGGCTTCTGAGCCTATCGGACATGATCGCAACGGCGCAACGGTTTATCTGAAAGATATCTGGCCATCGGcc                                     >  2:237452/1‑250 (MQ=255)                                                                                                                                                                                             tGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGcac                                    >  1:84171/1‑251 (MQ=255)                                                                                                                                                                                               ggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACaa                                  <  1:4078/251‑1 (MQ=255)                                                                                                                                                                                                gCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCCTGATCGCAAAGGCGATCCGGGTTATCTGAAAGATATCTGGCCATCGGcac                                    >  2:71934/1‑248 (MQ=255)                                                                                                                                                                                                gCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGcac                                    <  1:71934/248‑1 (MQ=255)                                                                                                                                                                                                           aTCCTATCGAAACGGCCATCAAACCAGGCGATTTAACCGTCGGTGCGGTGCTTTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTCAAACTAAAAGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAAAAGGGATATCAACCAGGCTTCTGAGGCTATCCGCCCTGTGAGCAAAAGCGAAACGGGTTTTCTGAAAGATATCTGGCCATGAGCACAAGACATTGCCCgg                      >  2:289460/1‑250 (MQ=255)                                                                                                                                                                                                                              cAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTTTCGGCCAt                                                                                     >  2:138/1‑169 (MQ=255)                                                                                                                                                                                                                              cAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCAt                                                                                     <  1:138/169‑1 (MQ=255)                                                                                                                                                                                                                               aaaaaagGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCcac  <  2:52821/251‑1 (MQ=255)                                                                                                                                                                                                                                 aaaagGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTCACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCcac  >  1:179948/1‑249 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     GCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCAC  >  NC_000913/1337166‑1337638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑