Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,205	C→A	T29K (ACG→AAG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,205	0	C	A	96.5%	98.3 / NA	29	T29K (ACG→AAG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (14/14);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTC  >  NC_000913/3485960‑3486441                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             cAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTGGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTa                                                                                                                                                                                                                                         <  2:235106/251‑1 (MQ=255)    tAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCttcttc                                                                                                                                                                                                                                      >  2:285262/1‑251 (MQ=255)          aTTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAgg                                                                                                                                                                                                                                <  1:189917/251‑1 (MQ=255)                       aTGTATGCAAAGGACGTCACATTAAAGTGCAGTACAGATGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGACTGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGGGAAAAAGCgg                                                                                                                                                                                                                    >  2:343093/1‑250 (MQ=255)                         gTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGaa                                                                                                                                                                                                                  <  1:296905/250‑1 (MQ=255)                             gCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCt                                                                                                                                                                                                             <  1:139793/251‑1 (MQ=255)                                    aCGTCACATGACCGTGCAGTACAGTTGATAGCCCCTGCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGGCAGCGGCGTTATCTGGCTCGGGATAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAATCCGCAAAGGGACCCGACGCTCGAATGGTTCTTGGCTCATTTCCACATGCATAAGTACCCATCCAAGAGCAAGCTTAGTCGCCAGGGTGAAAAAGCGGAAACGCTGaactac                                                                                                                                                                                                      <  2:144659/251‑1 (MQ=255)                                            ttACCGAGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCACTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACAGCAAACAGACCCGACTCTCGAAAGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGACACGCTGTACTACATAGTTa                                                                                                                                                                                               >  2:229813/1‑250 (MQ=255)                                                                tAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTATCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTg                                                                                                                                                                           >  1:141380/1‑250 (MQ=255)                                                                                            atTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACATACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGTAAACGCTGTACTACATCGTGAAAGGCTCGGTGGCAGTGCTGATCAAAGACTAAGAGGGTAAAGAAATGAt                                                                                                                                              <  2:314681/251‑1 (MQ=255)                                                                                                cGGCAATCCAGAGAAAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGAACctc                                                                                                                                          <  1:187862/251‑1 (MQ=255)                                                                                                       ccAGACACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTAAACCAGAGGATAACCGTGTATGGTGCTGGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTTCCACTGTAATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGTTGAAAAAGCGGAAACGCTGTACGACATCGTGAAAGGATCTGTGTCAGTGCTTATCAAAGACGAAGAGTGTAAAGAAATTATCCGCTCCTATc                                                                                                                                   <  2:344262/251‑1 (MQ=255)                                                                                                             aCAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATc                                                                                                                             <  1:43009/251‑1 (MQ=255)                                                                                                                 cggcgTTATCTGGCTCTGGAAAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAggg                                                                                                                         <  1:285262/251‑1 (MQ=255)                                                                                                                            ggCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTa                                                                                                                 <  1:56295/248‑1 (MQ=255)                                                                                                                                  ggAGAAGGCTTAAAACAAAGGATAACCGCGCATGGTGCTTGGAAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCGCTCCTATCTGAATCAGGGTGATTTTATTGGCGAAc                                                                                                        <  2:275431/251‑1 (MQ=255)                                                                                                                                                                   ggTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAgcg                                                                       >  1:274246/1‑251 (MQ=255)                                                                                                                                                                                       cAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTAGTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAAcc                                                   <  2:234380/251‑1 (MQ=255)                                                                                                                                                                                        aGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCg                                                  >  1:66381/1‑251 (MQ=255)                                                                                                                                                                                        aGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATAAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGGGCGAAAACCg                                                  >  2:204801/1‑251 (MQ=255)                                                                                                                                                                                              gACTCGCGAAGGGTTCTTGTCTCATTGCCACATTCATAAGTCCCCATCCAAGAGCACGCTTATTCCCCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACAAAGAGGGTAAAGAAAGGACCCTCTCCTATCGGAATCGGGTTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTCCGTGCGAAATCCGCCtgtg                                            <  1:117365/251‑1 (MQ=255)                                                                                                                                                                                                           ttCTTGTCTCATTGCCACCTTCATACGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGACTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGACTGTGAAGTGGCTGAAAt                               >  2:152236/1‑251 (MQ=255)                                                                                                                                                                                                                 tctcATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAtt                                                                                                                    >  1:210200/1‑160 (MQ=255)                                                                                                                                                                                                                 tctcATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACGACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAATACGAAGAGGGTAAAGAAATGATCCTCCCCTATCTGAATCAGGGTGAtt                                                                                                                    <  2:210200/160‑1 (MQ=255)                                                                                                                                                                                                                     aTTGCCACCTTCATAAGTACCCATCCAAGAGCAAGCTTATTCCCCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAAGGTGATTTTATTGGCGAACTGGGCCTGTTTGCAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACaa                      >  2:217031/1‑250 (MQ=255)                                                                                                                                                                                                                           acaTTCATAAGAACCCATCCACGAGCAAGCTTTTTCCCCAGGCTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTCTTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACTTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGACTGAACTTTCGTACAACAAAttt               >  2:218329/1‑251 (MQ=255)                                                                                                                                                                                                                                    aGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTg      >  2:349117/1‑251 (MQ=255)                                                                                                                                                                                                                                      tACCCATCCCAGAGCAAGCTTATTACCCCGGGAGAAAAAGCGGACACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAACGAAATGATCCTCTCCTCTCTGAATCAGGGTGATTTTCTTGGCGAACTGGGCCTGTTTGAAGAGGGCAAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGCAGTGGCTGAAATTTCGTACagatcggaagcgggtcgtgg    >  2:201254/1‑232 (MQ=255)                                                                                                                                                                                                                                          cATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTc  >  1:122778/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             CAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTC  >  NC_000913/3485960‑3486441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑