Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,219	A→G	L258P (CTA→CCA)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,219	0	A	G	100.0%	59.5 / NA	20	L258P (CTA→CCA)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/10);  total (10/10)

CTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGG  >  NC_000913/4311984‑4312447                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                     cattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGTTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGc                                                                                                                                                                                                                      <  2:130394‑M2/178‑1 (MQ=255)    tccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCTGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCg                                                                                                                                                                                                                    <  1:191848‑M2/180‑1 (MQ=255)                        atttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTGCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAATGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACg                                                                                                                                                                                               <  1:218794‑M2/201‑1 (MQ=255)                                                          gtaagttttgaggtgaGGTAAGGGGACA‑CTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCCAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATc                                                                                                                                                             >  2:132046‑M2/17‑251 (MQ=255)                                                              gttttgacgtgaGGTAAGGGGCCA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGCCAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGatcgtcc                                                                                                                                                         >  2:13409‑M2/13‑248 (MQ=255)                                                                     cgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAAGATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCg                                                                                                                                                  <  1:207685‑M2/246‑1 (MQ=255)                                                                           gTAAGGGGACAG‑TAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTGTTTCCATATTAATATTAGCTTGTTCAATGTTGGGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGGAATTTTGTTGCCGATGCATTACGACCTAATAGCGTCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCg                                                                                                                                            <  1:140880/251‑1 (MQ=255)                                                                                          aCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGCAGCAATCAACAATTGCGCCGGTTTTTTCCATATTATTATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCt                                                                                                                               >  2:153563/1‑250 (MQ=255)                                                                                                                   ttcgtATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTTCGCCGGTTTTTTACATATTAAGATTAGCTTGTTCAATGTTGAGCTTGGCGACAGAGACAAAAAAAGCGTCCATCAAGGTTGTTTGTAATATTCTTGCCGAGGAATTACGACCTAATAACGGCGTTTCCGGGGCTTGTTAGCAAATAATATGATCTGCCAGTTTCGCTATCGGTTAGGGGTAGCTATTGTTTATACAAATAAGCTTTGcc                                                                                                      <  2:218129/246‑1 (MQ=255)                                                                                                                        aTTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTc                                                                                                <  1:77328/251‑1 (MQ=255)                                                                                                                                      ggTGAGAAGAACTCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCAGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGttt                                                                                  >  1:215279/1‑250 (MQ=255)                                                                                                                                        tGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCt                                                                                 >  1:163096/1‑250 (MQ=255)                                                                                                                                              gaaATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGCGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCg                                                                          >  2:41970/1‑251 (MQ=255)                                                                                                                                                                   tttttCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTATACGGCCGCTTTTACATCACTGGTTCGcc                                                     >  2:316105/1‑251 (MQ=255)                                                                                                                                                                          atattaatattaGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATg                                              >  1:10394/1‑251 (MQ=255)                                                                                                                                                                                                    gAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATAtcctt                    >  1:153298/1‑251 (MQ=255)                                                                                                                                                                                                        ggggCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTg                <  1:41970/250‑1 (MQ=255)                                                                                                                                                                                                           gCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTaa             <  2:288858/251‑1 (MQ=255)                                                                                                                                                                                                                   gaCAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAg     <  1:167232/251‑1 (MQ=255)                                                                                                                                                                                                                       aaaaaaGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACATAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACACCGAAGCgg  >  1:112626/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                     CTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGG  >  NC_000913/4311984‑4312447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑