breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 144 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913581,3350CA62.9% 159.4 / 257.1 222L168M (CTG→ATG) ‡nohDputative DNA‑packaging protein NohD
*NC_000913581,3220CT62.6% 260.9 / 224.6 214A163A (GCC→GCTnohDputative DNA‑packaging protein NohD
*NC_000913581,3370GA62.3% 252.7 / 244.2 223L168L (CTG→CTA) ‡nohDputative DNA‑packaging protein NohD
*NC_000913581,3810AG60.5% 153.4 / 269.3 211intergenic (+2/‑153)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,3800CG59.7% 139.3 / 293.2 212intergenic (+1/‑154)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,2910GA56.6% 95.0 / 196.7 168R153Q (CGG→CAG) ‡nohDputative DNA‑packaging protein NohD
*NC_000913581,2750GA56.3% 127.4 / 224.7 167V148I (GTT→ATT) nohDputative DNA‑packaging protein NohD
*NC_000913581,2890AG55.5% 62.9 / 229.5 166K152K (AAA→AAGnohDputative DNA‑packaging protein NohD
*NC_000913581,2900CA55.4% 50.2 / 231.3 167R153R (CGG→AGG) ‡nohDputative DNA‑packaging protein NohD
*NC_000913581,2950TC55.3% 87.5 / 252.0 170D154D (GAT→GACnohDputative DNA‑packaging protein NohD
*NC_000913581,3010CT55.0% 103.9 / 229.0 169I156I (ATC→ATTnohDputative DNA‑packaging protein NohD
*NC_000913581,2860GC54.9% 92.6 / 243.0 166L151L (CTG→CTCnohDputative DNA‑packaging protein NohD
*NC_000913581,2500TC54.7% 73.7 / 223.7 148R139R (CGT→CGCnohDputative DNA‑packaging protein NohD
*NC_000913581,2590AG54.7% 81.6 / 211.4 151E142E (GAA→GAGnohDputative DNA‑packaging protein NohD
*NC_000913581,2530TC54.4% 31.9 / 221.4 148F140F (TTT→TTCnohDputative DNA‑packaging protein NohD
*NC_000913581,4040CG53.5% 45.0 / 288.4 174intergenic (+25/‑130)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,3940TC53.2% 20.7 / 272.3 173intergenic (+15/‑140)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,4120GT53.2% 4.8 / 268.0 174intergenic (+33/‑122)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,4130CT53.2% 52.2 / 258.0 174intergenic (+34/‑121)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,4160AG53.2% 24.6 / 268.0 173intergenic (+37/‑118)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD

Marginal new junction evidence (lowest skew 10 of 35 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4542682 =147 (1.560)10 (0.110) 8/566 6.3 7.2% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 117 (1.280)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 1207800 =33 (0.350)8 (0.080)
+A
8/582 6.4 17.2% coding (300/630 nt) ycfK protein StfP
?NC_000913 1209610 = 44 (0.470)intergenic (‑491/‑75) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 781369 =86 (0.910)13 (0.140)
+G
8/582 6.4 16.1% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521127 50 (0.530)intergenic (+1/‑46) valX/valY tRNA‑Val/tRNA‑Val
* ? NC_000913 781369 =86 (0.910)13 (0.140)
+GG
8/580 6.4 14.5% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 68 (0.720)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 69650670 (0.740)6 (0.080)
+45 bp
6/494 6.4 8.3% intergenic (‑2/+36) glnX/glnV tRNA‑Gln/tRNA‑Gln
?NC_000913 697057 = 86 (0.910)noncoding (77/77 nt) metT tRNA‑Met
* ? NC_000913 = 69645475 (0.790)10 (0.110) 7/562 6.6 12.7% noncoding (51/75 nt) glnX tRNA‑Gln
?NC_000913 696890 = 65 (0.710)noncoding (50/75 nt) glnU tRNA‑Gln
* ? NC_000913 3270072 =61 (0.650)5 (0.060)
+25 bp
5/534 7.3 6.6% intergenic (+470/+144) yhaC/rnpB uncharacterized protein YhaC/RNase P catalytic RNA component
?NC_000913 4137788 = 94 (0.990)intergenic (‑131/+144) yijF/gldA DUF1287 domain‑containing protein YijF/L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
* ? NC_000913 = 2653389105 (1.110)5 (0.050) 5/574 7.7 4.8% noncoding (67/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653716 = 94 (1.010)intergenic (‑261/‑139) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 = 128689275 (0.790)5 (0.050)
+C
5/582 7.7 6.3% coding (184/276 nt) ychS putative uncharacterized protein YchS
?NC_000913 1287544 = 75 (0.790)noncoding (79/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 78091870 (0.740)6 (0.070) 4/548 7.9 7.8% noncoding (76/76 nt) lysW tRNA‑Lys
?NC_000913 781321 = 76 (0.860)intergenic (+99/‑48) lysY/lysZ tRNA‑Lys/tRNA‑Lys