breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 37 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913847,9550GA38.0% 144.7 / 79.2 143A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_00091358,0650CA35.7% 120.6 / 16.1 84P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009133,741,8710GT33.9% 184.1 / 17.0 118S221Y (TCC→TAC) plaRDNA‑binding transcriptional repressor PlaR
*NC_0009131,611,5070GT32.6% 117.2 / 10.9 86L116L (CTC→CTAdgcFputative diguanylate cyclase DgcF
*NC_0009133,911,5330CA32.4% 220.1 / 13.8 136intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009132,465,0160CA32.1% 178.2 / 13.7 106intergenic (‑9/‑285)mlaA/yfdCintermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA/inner membrane protein YfdC
*NC_0009131,466,4380TG31.8% 98.4 / 41.4 63pseudogene (1047/2518 nt)ydbAputative outer membrane protein, N‑terminal fragment
*NC_0009132,767,4450CA30.9% 181.8 / 14.3 81intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009132,477,2650AC30.0% 164.1 / 16.1 90L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009131,351,4630CA29.7% 137.7 / 11.0 91E100* (GAG→TAG) ycjDDUF559 domain‑containing protein YcjD
*NC_0009134,074,5720CA29.5% 195.7 / 11.5 112P278P (CCC→CCAyihV6‑deoxy‑6‑sulfofructose kinase
*NC_0009132,326,7130CA29.4% 183.1 / 12.6 102P202Q (CCG→CAG) atoBacetyl‑CoA acetyltransferase
*NC_0009131,466,4320AG28.6% 119.3 / 36.5 63pseudogene (1041/2518 nt)ydbAputative outer membrane protein, N‑terminal fragment
*NC_0009132,990,4800CA28.4% 263.9 / 15.6 148intergenic (‑120/‑788)yqeK/ygeGprotein YqeK/TPR repeat‑containing putative chaperone YgeG
*NC_0009134,261,0400CA27.6% 257.9 / 11.1 127Q148K (CAA→AAA) yjbMDUF2713 domain‑containing protein YjbM
*NC_0009133,768,4350CA27.5% 328.9 / 13.5 160Q87K (CAG→AAG) yibGtetratricopeptide‑like domain‑containing protein YibG
*NC_0009132,270,1380CA27.4% 219.8 / 11.7 113Q54K (CAG→AAG) mepSpeptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
*NC_0009131,097,1180CA27.1% 69.2 / 40.5 59intergenic (+289/+447)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009131,466,4180AG27.0% 135.3 / 33.4 63pseudogene (1027/2518 nt)ydbAputative outer membrane protein, N‑terminal fragment
*NC_0009132,178,1560CA26.7% 200.9 / 14.4 101E137* (GAG→TAG) fbaBfructose‑bisphosphate aldolase class I

Marginal new junction evidence (lowest skew 10 of 28 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 128708464 (0.710)13 (0.160) 9/546 5.3 17.8% noncoding (153/171 nt) rttR small RNA RttR
?NC_000913 1287557 = 60 (0.720)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 128690657 (0.630)10 (0.120) 9/546 5.3 15.0% coding (198/276 nt) ychS putative uncharacterized protein YchS
?NC_000913 1287557 = 60 (0.720)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 3764827NA (NA)10 (0.120) 9/562 5.4 72.3% coding (2645/4134 nt) rhsA rhs element protein RhsA
?NC_000913 3765008 = 4 (0.040)coding (2826/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 73223971 (0.790)10 (0.120) 9/562 5.4 21.7% coding (2657/4194 nt) rhsC rhs element protein RhsC
?NC_000913 3765020 = 4 (0.050)coding (2838/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 294746747 (0.520)6 (0.090) 5/420 5.6 14.7% intergenic (+4/‑30) metZ/metW tRNA‑Met/tRNA‑Met
?NC_000913 2947688 = 36 (0.560)intergenic (+5/+69) metV/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
* ? NC_000913 = 69674161 (0.680)5 (0.080) 5/430 5.7 8.7% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 60 (0.910)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 3621836NA (NA)9 (0.100) 8/562 5.8 82.4% coding (2645/4236 nt) rhsB rhs element protein RhsB
?NC_000913 3622017 = 2 (0.020)coding (2826/4236 nt) rhsB rhs element protein RhsB
* ? NC_000913 = 73223971 (0.790)9 (0.100) 8/562 5.8 20.4% coding (2657/4194 nt) rhsC rhs element protein RhsC
?NC_000913 3622029 = 2 (0.020)coding (2838/4236 nt) rhsB rhs element protein RhsB
* ? NC_000913 4542682 =159 (1.770)9 (0.100) 7/568 6.2 6.1% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 122 (1.400)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 251811672 (0.800)5 (0.080) 4/424 6.2 8.8% noncoding (1/76 nt) alaX tRNA‑Ala
?NC_000913 2518232 = 52 (0.800)intergenic (‑1/‑235) alaW/yfeC tRNA‑Ala/DNA‑binding transcriptional dual regulator YfeC