breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 34 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,911,5330CA36.6% 175.5 / 27.4 123intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009131,427,9920GT35.4% 132.0 / 15.8 113P245P (CCC→CCAinsH5IS5 family transposase and trans‑activator
*NC_0009132,073,6130GT35.3% 123.5 / 19.2 86R692L (CGG→CTG) fluself recognizing antigen 43 (Ag43) autotransporter
*NC_0009133,054,8940GT34.5% 136.1 / 26.8 88P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_00091358,0650CA33.0% 150.5 / 10.8 88P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009133,054,8860GT32.2% 147.1 / 27.0 88Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_0009132,472,8170CA32.0% 132.2 / 13.2 78G22V (GGA→GTA) yfdNuncharacterized protein YfdN
*NC_0009134,136,4670AC31.8% 105.8 / 18.6 63E120D (GAA→GACyijEcystine exporter
*NC_0009131,334,9660AC31.2% 248.8 / 34.8 160intergenic (+137/‑182)cysB/ymiADNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA
*NC_0009132,767,4450CA31.0% 246.7 / 10.9 113intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009131,922,8150CA30.5% 147.1 / 23.8 105G52V (GGA→GTA) pphAphosphoprotein phosphatase 1
*NC_0009132,288,4180GT30.2% 225.6 / 32.1 139pseudogene (497/2525 nt)yejOadhesin‑like autotransporter YejO
*NC_0009132,477,2650AC28.6% 188.6 / 24.2 98L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009133,543,2200AC28.4% 175.9 / 10.0 109L18F (TTA→TTCrpnArecombination‑promoting nuclease RpnA
*NC_0009132,465,0160CA28.1% 218.5 / 10.7 121intergenic (‑9/‑285)mlaA/yfdCintermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA/inner membrane protein YfdC
*NC_0009131,579,9730CA27.7% 267.9 / 14.0 137G273V (GGA→GTA) ydeMputative anaerobic sulfatase maturation enzyme YdeM
*NC_0009134,296,1910AC27.3% 37.2 / 28.6 44intergenic (+397/+245)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_000913847,9550GA27.2% 227.4 / 48.5 136A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_000913161,5490AC27.2% 159.2 / 11.4 81R161R (CGT→CGGthpRRNA 2',3'‑cyclic phosphodiesterase
*NC_0009132,457,1770TG26.8% 95.5 / 10.4 57G661G (GGA→GGCfadJ3‑hydroxyacyl‑CoA dehydrogenase FadJ

Marginal new junction evidence (lowest skew 10 of 15 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 273955 =NA (NA)17 (0.170) 15/598 5.2 13.4% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 569148 110 (1.130)coding (247/1527 nt) ybcK putative recombinase YbcK
* ? NC_000913 = 78122250 (0.510)11 (0.150) 8/446 5.8 21.5% noncoding (76/76 nt) lysY tRNA‑Lys
?NC_000913 781653 = 43 (0.590)intergenic (+1/‑432) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 275149NA (NA)12 (0.120) 11/598 6.3 9.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 569145 = 113 (1.160)coding (244/1527 nt) ybcK putative recombinase YbcK
* ? NC_000913 = 3648146118 (1.210)7 (0.090) 7/482 6.7 7.2% intergenic (+2/‑148) agrA/agrB small RNA AgrA/small regulatory RNA AgrB
?NC_000913 3648378 = 85 (1.080)intergenic (+3/‑150) agrB/arsR small regulatory RNA AgrB/DNA‑binding transcriptional repressor ArsR
* ? NC_000913 = 78132260 (0.610)7 (0.080) 7/520 7.1 10.4% intergenic (+100/‑47) lysY/lysZ tRNA‑Lys/tRNA‑Lys
?NC_000913 781811 = 68 (0.800)intergenic (+159/‑274) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 4606166101 (1.030)5 (0.070) 4/420 7.4 7.2% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 57 (0.830)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 78144649 (0.500)3 (0.040) 3/442 8.3 7.0% intergenic (+2/‑131) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
?NC_000913 781655 = 43 (0.600)intergenic (+3/‑430) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 184226 =99 (1.020)5 (0.050) 5/596 8.8 4.8% intergenic (‑131/+31) yaeH/yaeI DUF3461 domain‑containing protein YaeH/phosphodiesterase YaeI
?NC_000913 270541 = NA (NA)noncoding (1/1221 nt) IS30 repeat region
* ? NC_000913 = 460628675 (0.770)3 (0.040) 3/504 9.1 4.0% noncoding (1/87 nt) leuP tRNA‑Leu
?NC_000913 4606402 = 79 (0.960)intergenic (‑1/+267) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 265339088 (0.900)5 (0.050)
+A
4/596 9.4 5.4% noncoding (66/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653718 = 87 (0.890)intergenic (‑263/‑137) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA