| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 161,549 | 0 | A | C | 27.2% | 159.2 / 11.4 | 81 | R161R (CGT→CGG) | thpR | RNA 2',3'‑cyclic phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base A (13/46); new base C (22/0); total (35/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.97e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑ACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAG > NC_000913/161270‑161848 | ggCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGGGTGTGAGTCCACAGGACAAA‑GAGGAGGGGTa > 1:285828/1‑300 (MQ=255) tttGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGTTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGTAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGTAGGCGTAAAg < 1:509621/300‑1 (MQ=255) tttGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAg < 1:246694/300‑1 (MQ=255) ttaGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGTTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGACCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAggg < 2:655095/298‑1 (MQ=255) tGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAggg < 1:579598/300‑1 (MQ=255) aTTCCCAGGTGTGTGGTTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTc < 1:425326/300‑1 (MQ=255) aTTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGTGTGTGTGTCCCC‑AGGCAAAAGAGGAGGAGTCAAggggggaacc > 1:83303/1‑293 (MQ=255) aGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAANTCGCGACCAGCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTTCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCAc < 1:568127/300‑1 (MQ=255) aGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGGAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCAc < 1:163167/300‑1 (MQ=255) gtgtgtGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTCACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGAGTGTGCGTCCAC‑AGGAAAACAAGGAGGGATAAAGGGTggaccccgcacaca > 1:302263/1‑287 (MQ=255) gggTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGAGTGTGTGTCCCC‑GGGAAAAAAAGGAGGCATGAAGGGTGGACTCCATCACCGCACACg > 1:509847/1‑300 (MQ=255) gTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑c < 1:609753/300‑1 (MQ=255) tATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTGGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑cc < 1:141454/300‑1 (MQ=255) ttGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCTCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAAc < 2:275568/300‑1 (MQ=255) tGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTNGCGCGCTGTAGAGGGGGAGAAAATTCNATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAAcc < 2:528042/300‑1 (MQ=255) tGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAGTCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAAcc < 2:702962/300‑1 (MQ=255) tGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGCTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAAcc < 1:713632/300‑1 (MQ=255) gtgtTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCCC‑AGGCAAACAAGGAGGAGTAAAGGGTGAACCCCATCACCGCACACAA‑CCAGTT‑C‑AAACCTg > 1:456416/1‑300 (MQ=255) gtgtTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATNGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGAGGGTGCGTCCAC‑AGGAAAACAAAGGGGAGTAAAGGGTGGACTCCGttcccacatccaaacaattcaaaacct > 2:677495/1‑274 (MQ=255) gtCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAACGTTTTAGCGGCGTGTAGAGTGTTCGTCCCC‑AGGAAAAAGAAGAGGCGTCAAGGGTGAACTCCATCACCACAGACGA‑ACAggtcaaaaccggcaga > 1:341094/1‑285 (MQ=255) aTACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTTTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GTGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑Acc < 2:394739/300‑1 (MQ=255) gTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGCGTGTGCGTCCAC‑GGGCAAACAAAGAGGCGTAAAAGGTGAAATCCGTCACCGCATACAA‑CCCGTT‑C‑AAACCttgtggggggggtgttacctaatcgcctg > 2:433949/1‑272 (MQ=255) tCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGt < 1:709944/300‑1 (MQ=255) cGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGGGCGTGTGCGTCCCC‑GGGCAAAAAAAGAAGCGTAAAAGGGGGACTCCGTCCCCACACACAA‑ACAATT‑A‑AAAACCAGCGGCGGGGGTGTC‑CACGCCCCGCTGGCGTc > 1:396907/1‑300 (MQ=255) ttGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGNGAAAATTCCATTGCGACAATCCTTGTNATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTCACATGTGAGTCCCC‑AGGCAAACAAAGAGGAGTAAAGGGTGAAATCCCTCACCTCATCCAA‑CCAGTC‑G‑CAAACTTGAGGGGGGGTTGTT‑ACCGCCTCCCTGGTGTCGCGCaacaa > 2:511090/1‑297 (MQ=255) ttGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGGCAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGTGTCCAC‑AGGAAAACGAGGGGGAGTAAAGGGTGAACTCCATCCCCACACACGA‑ACAATT‑C‑AAACCCGGCGGGGGGGTTGTC‑CCCACCCCGCTTGCGTctcgtcacaa > 1:55750/1‑291 (MQ=255) cacaACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGGGATCACATCTGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGTAGAAAATTCCATTTCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCATGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGt < 1:697978/300‑1 (MQ=255) caccctgccATCGCACCCGTATTCGTGCAGTGTAGCGTTAATTCGCTACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTaa < 2:711580/292‑1 (MQ=255) cgttCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATAtg < 2:66715/297‑1 (MQ=255) cgttCATCGCACCCGTAGTCGGGCAGTGTGGCGTTAATGCGCGAACATCGGGGGTGATCACATCGGCTAAAAAAAGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGTAGAAAATTCCATTTCGACAATCCTTGTTATTGCGTTAACGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GTGCAAACGAGGAGGCGTAGAGGGGGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGAGTCGCGCAATAAGGTAATAtg < 2:242436/297‑1 (MQ=255) cattCATCGCACCCATATTCGTGCAGTTTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACATTTTCAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTGTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAatagg < 2:214962/297‑3 (MQ=255) cagTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACAGCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGNGGGGTAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGGTTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATAtg < 1:526548/298‑1 (MQ=255) cGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATAtg < 1:263177/300‑1 (MQ=255) gTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGAc < 1:166565/300‑1 (MQ=255) gtgtAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGTGTGTGTGTCCAC‑GGGAAAAAAAGGAGGAGTAAAGGGTGAAACCCATCCCCGCACACAA‑ACAGTT‑C‑AAACCCGGAGGAGGGGTTGTT‑ACCACCTCGCTGGCGTCGCGAAATGAGGGAAAATGTGGATGAAACGGACAATTGCtttta > 1:437577/1‑298 (MQ=255) gtgtAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGTGTCCCC‑GGGCAAACGAGGAGGAGTAAAAGGTGAAATCCATCACCGCATCCGA‑CCCATT‑C‑AAACCTTGCGGCGGGGTTGTC‑CCCACCCCGCTTGCGTCACGCAACAAGGTAATCTGTGGATGAAAAAGACGAttttttttg > 1:633211/1‑293 (MQ=255) gtgtAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCCC‑GGGCAAACAAGGAGGCGTCAAGGGTGAACCCCGTCCCCGCATCCGA‑CCCATT‑C‑AAACCCTGCGGAGGGGTTGTC‑CCCACCCCGCTGGTGTCGCGCAATAAAGTAATACGTTGATGAAACAGACGATTGCtttta > 1:464168/1‑298 (MQ=255) gtgtAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCCC‑AGGCAAACAAGGAGGCGTAAAGAGTGAACTCCGTCCCCCCCCCCAC‑CCAATG‑A‑ACACCTTGCGGCGGGATTGTC‑CCCACCTCCCTGGCGTTTCGCAACAAGGTAATATGTGGGTGAAACAGACAATTTCTTTgc > 2:467403/1‑299 (MQ=255) gtgtAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGAATGTGCGTCCCC‑AGGCAAACAAGGAGGCGTAAAGGGTGAACCCCGTCACCACCCACAA‑CCCATT‑A‑CAACCTGGCGGCGGGGTTGTT‑CCCCCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAAAGGACGGTTGttttta > 1:5236/1‑298 (MQ=255) gACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑c < 2:5231/300‑1 (MQ=255) ggTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGTGTCCCC‑AGGAAAACGAGGAGGCGTAAAGGGTGAACCCCGTCCCCACCTCCAA‑CCAATT‑C‑AAACCTGGCGGTGGGAGTGTT‑CCCACCTCGCTGGTGTTGCGCAATAAGGTAATACGTGGATGCAACGGACAATTGCTTTTGAAACAACCGCTGCGGG‑TAGACtgtg > 1:23359/1‑300 (MQ=255) tgtgATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGaa < 1:581739/300‑1 (MQ=255) tgtgATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGAATGTTCGTCCCC‑GGGCAAAAGAGGAGGCATAAAGGGTGGACCCCCTCCCCACATACAA‑CCCATN‑C‑AAACCTTGCGGCGGGATTTTC‑CCCTCCTCGCTGGCGTTTTGCAATAAGGTGATATGTGGATGAAAAGAACAATTTTTTTGAAAAAAAACACTTCGGG‑TAGCCTGTGaa > 1:530784/1‑300 (MQ=255) gtgATCACATCGGCGAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAAc < 1:746709/300‑1 (MQ=255) gATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGTGTCCCC‑AGGAAAACAAGGAGGAATAAAAGGTGGACTCCGTCCCCGCACCCAA‑ACAATT‑C‑ANACCCGGAGGTGGGATTGTC‑CCCACCTCGCTGGGGTTGCGAAATAAGGGAATATGTGGATGAAACAGACGATTGCTTTTAAAAAAACCGCTGCGGG‑AAGACTGTGAAcag > 1:547405/1‑298 (MQ=255) gATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGANTGTGTTTCCCC‑AGGGAAAAAAGGAGGNGTAAAAGGTGAACTCCATCACCACACACGA‑CCCATT‑ACAAACCTGG‑GGAGGGGTTGTC‑CCCCCCTCTCCTGTGTTGCGCCACGAGGTAATATGTGGAGGCAAAAGAAGATTTCTTTTCAAACAACCAATGCGGG‑CAGCCtgtggaagg > 2:508447/1‑295 (MQ=255) cacaTCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGc < 1:209661/300‑1 (MQ=255) cGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATAtt < 1:474768/300‑1 (MQ=255) tAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGTTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGc < 1:748754/300‑1 (MQ=255) tAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGc < 1:257683/300‑1 (MQ=255) tAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGc < 2:602087/300‑1 (MQ=255) gAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCCC‑AGGCAAACAAGGAGGAGTAAAGGGTGAACCCCATCCCCGCATCCAA‑ACAATT‑C‑AAACCCGGCGGCGGGGTTGTC‑CCCGCCTCGCTGGTGTCGCGCAACAAGGTAATATGTGGATGAAAAAGACAACTTCTTTTCAAACAACCACCGCGGG‑CAGACTGTGAACGGAGCATACTCTCCAACTGGATTCAGCCACGTgg > 1:690052/1‑300 (MQ=255) gAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCCC‑AGGAAAACAAGGAGGAATAAAAGGTGAACTCCCTCACCAACCCCAA‑CCAATT‑C‑AAACCCTGCGGAGGGATTGTCCACC‑CCTCACCTGCGTTGCGCAATAAGGTAATATGTTGATGAAAAAGACGATTTCTTTTAAAACCACCCCTTCGGG‑CAGCCCTTTAACGGAGCatcttttccaacctgattcagccacgtgg > 1:741222/1‑273 (MQ=255) cTGTAGAGGGGGAGAAAATTCCATTGCGACANTCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGTGTTCCC‑GGGAAAACGAGGGGGCGTAAAGGGTGGACTCCGTCCNCGCATACGA‑ACAATT‑A‑AAACCTTGCGGAGGGATTGTC‑ACCGCCTCGCTGGCGTTGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGATGCGGG‑AAGCCTTTGAACGGAGCATCTTCTCCCGCTGGATTCCGCCGCGTGGTGGCTGACGGATCCCt > 2:536025/1‑300 (MQ=255) cTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCCC‑AGGCAAAAGAGGAGGAGTAAAAGGTGAACCCCGTCACCGCACACGA‑ACAGTTGA‑AAACC‑GGAGGCGGGGGTGTC‑CCCGCCCCGCTGGCGTCGAGCCACAAGGTAATATGTGGATGAAACGGACGATTTCTTTTAAAAAAACCACTGCGGG‑CAGCCTGTGAACAGAGCATATTTGCCAGCTGGATTAAGCCGCGTGGCGGTTGACGCATCCCt > 1:489943/1‑300 (MQ=255) cTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCCC‑AAGCAAACAAGGAGGAGTAAAAGGTGAACCCCTTCACCACACCCAA‑CCAGTT‑A‑AAACCTGGTGGAGGGATTTTC‑ACCTCCTCACCGGCGTCGCGCAATAAGGTAATATGTGGATGAAACAGACGATTGCTTTTAAAACCACCGCTTCTGG‑CAGCCTTTGAACGGAGCATATTCTCCAACTGGATTCAGCCGCGTTGCGGCTTACGccccccc > 1:510770/1‑294 (MQ=255) cTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGCGTGTGCGTCCCC‑GGGAAAACAAGGGGGAGTAAAGGGTGGACCCCGTCACCACACACAAACCA‑TT‑A‑AAACCTTGCGGCGGGGTTTTC‑ACCGCCTCGCTTGCGTCGCGCAACAAGGTAACATGTGGATGAAACGGACGATTGCTTTTAAAACCACCACTGCGGG‑CAACCTGTGAACGGAGCATATTCTCCAACTGGATTAAGCCGCGTGGCGGCTGACGCATCCCt > 1:411684/1‑300 (MQ=255) cTGTAGAGGGGGAGAAAATTCCATTGCGACAATCATTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGGACTCCGCCACCATATACGA‑CCCGCT‑A‑AAACCTGGGGGTAGGGTTGTC‑CCCGCCCCTTTGGCGTTTCGCAATGAGGTAATATGTGGAGGAAACGGACGATTGCTTTTAAAACAACCACTGCGGGTGAACC‑GTGAACAGAGCATATTTTCCAGCCGGATTAAGCCTTGTGGAGGTTTACGCATcccc > 2:699850/1‑299 (MQ=255) agagTGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCNCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATNAAGCCGCGTGGCGGCTGACGCATCCCTA‑acc < 2:562868/300‑1 (MQ=255) agagGGGGAGAAAATTCCATTGCGACAATCCTTTTTATTGCTTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑acc < 2:21965/300‑1 (MQ=255) agagGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTNAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑acc < 1:551833/300‑1 (MQ=255) agagGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑acc < 1:200462/300‑1 (MQ=255) agagGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑acc < 2:428115/300‑1 (MQ=255) agagGGGGAAAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCATCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGTAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑acc < 2:313276/300‑1 (MQ=255) agGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑accac < 1:53536/300‑1 (MQ=255) gagaAAATGCCATTGCAACAATCCTTTTTACTGCGGTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTTGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑accacaccac < 2:137131/300‑1 (MQ=255) aatgccATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑ACCACACCACAcgcg < 2:216007/296‑1 (MQ=255) aCAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAAGCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑accctgtctcttatacacatctcgcag < 1:425869/300‑25 (MQ=255) aTCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGGGTGTGCGTCCCC‑GGGAAAAAAAGGAGGCGTAAAAGGTGAACTCCGTCCCCGCATCCAA‑CCAATTCA‑AAACCTG‑CGGCGGGGTTTTC‑CCCACCCCGCTGGTGTTGTGAAATGAGGGAGTATGTGGATGAAACAGACGATTGTTTTTCAAAAAACCGCTTTGGG‑CCGCCTTTTGACGGAGCCTCTTTGCCCGCTTGGTTCAGCCACGTGGCGGTTGACGCCCCCCCC‑ACCCCACCACACTCGAACGCAGTCAttttt > 2:421770/1‑297 (MQ=255) cTTGTTATTGCGTTAGCGTCTAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑AGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAATGGACGATTGCTTTGAAAACAACCACTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑ACCACACCACACGTGAACGCAGCCATTGTCCgg < 2:339267/300‑1 (MQ=255) ttGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTTTTTGTCCAC‑AGGAAAACGAGGAGGAGTAAAAGGTGAACCCCATCCCCCAATACAA‑CCAGTT‑G‑AAACCCAACGGCGGGGTTNTC‑ACCACCTCGCCGGCGTCGCGCAATNAGGTAATCTGTGGAGGAAACAGACAATTTCCTTTCAAACAACCGCTGCGGG‑CAGTATGTGAACGGGGCATATTTGCCAACTTGAGTAAGACGCGTGGCGGTTGACGAATCCCTC‑AACACACCAAAcgggagcggagacagtgtcctgg > 2:521576/1‑279 (MQ=255) gTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTTTGCGTCCCC‑AGGAAAACAAGGAGGCGTAAAAGGTGAACTCCGTCACCACACCCGA‑CCAGTT‑A‑AAAACCGGAGGCGGGGTTTTC‑CCCACCCCGCTTGGGTCGCGCCACAAGGTCATATGTGGATGGAACGGACGATTGCTTTTAAAACAACCACCGCGGG‑CAGCCTTTGAACGGAGCCTCTTCTCCCGCTTGATTCAGCCACATGGCGGCTTACACCTCCCTCCACC‑CACCACACACGAACGCaaaccttttccagagt > 1:439636/1‑285 (MQ=255) ttAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGTAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑ACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCg < 2:745570/300‑1 (MQ=255) ttAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑ACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCg < 1:191228/300‑1 (MQ=255) aGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑ACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGt < 2:599430/300‑1 (MQ=255) aGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑ACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGt < 1:761833/300‑1 (MQ=255) gcgTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑ACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGAc > 1:342479/1‑300 (MQ=255) cgTGTAGCGTGTGGGTCCAC‑GGACAAACGAGGAGGCGTAAAGGGTGAACTCCGTCAACGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑AACGCCTCGCTGGTGTCGCGCAATAAGGTCATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCAAGCTGGATTAAGCCGCGTGGGGGCTGACGCCTCCCTA‑ACCACACCACACGCGAACGCAGCCGTTGTCCGGCGCCATCAAGGGTGGGTGTGAAACCAGGTTGACg > 2:273276/1‑300 (MQ=255) cgTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑ACCACACCACACGCGAACGCAGCAATTGTCCGGCGTCATCAAGCGTGAGTGTGAAAACAGGTTGACg > 2:71595/1‑300 (MQ=255) tgCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑ACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCgg > 2:767304/1‑300 (MQ=255) gTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACAA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑AACGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTAGCGGCTGACGCATCCCTA‑ACCACACCACACGCGGACGCAGCCATTGTCCGGTGTCATCAAGCGTGAGTGTGCAACCAGGTTGACGAAtccgtctggtta > 2:199296/1‑294 (MQ=255) aC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑ACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAag < 1:643021/300‑1 (MQ=255) | GGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCAC‑GGGCAAACGAGGAGGCGTAAAGGGTGAACTCCGTCACCGCATACGA‑CCAGTT‑A‑AAACCTGGCGGCGGGATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGG‑CAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTA‑ACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAG > NC_000913/161270‑161848 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |