breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 47 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,427,9920GT42.6% 115.8 / 35.0 129P245P (CCC→CCAinsH5IS5 family transposase and trans‑activator
*NC_0009133,054,8940GT40.0% 100.6 / 39.4 95P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_0009134,296,1910AC40.0% 9.5 / 20.5 30intergenic (+397/+245)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009132,433,3400GT39.7% 104.7 / 16.2 78S196Y (TCC→TAC) accDacetyl‑CoA carboxyltransferase subunit beta
*NC_0009133,911,5330CA37.1% 203.1 / 28.1 143intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009132,159,4130CT36.8% 63.5 / 24.5 57A343V (GCG→GTG) mdtCmultidrug efflux pump RND permease subunit MdtC
*NC_0009133,054,8860GT36.4% 140.4 / 33.9 100Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_0009132,477,2650AC36.0% 160.2 / 31.6 100L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009133,226,2240GT31.9% 235.3 / 17.7 135intergenic (+53/‑10)ebgC/ygjIDUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta/putative transporter YgjI
*NC_00091358,0650CA30.6% 179.1 / 14.1 108P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_00091312,1520GT30.2% 199.7 / 19.6 126intergenic (‑366/‑11)yaaI/dnaKDUF2541 domain‑containing protein YaaI/chaperone protein DnaK
*NC_0009131,823,0180CA30.1% 143.6 / 11.8 103L187L (CTC→CTAnadENH3‑dependent NAD(+) synthetase
*NC_0009134,296,1900AG30.0% 25.3 / 19.1 30intergenic (+396/+246)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009132,767,4450CA29.9% 200.4 / 21.8 120intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009132,767,4490CA29.8% 220.2 / 22.8 123intergenic (+94/‑261)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_000913866,3190CA28.7% 181.7 / 10.4 122E16* (GAG→TAG) moeAmolybdopterin molybdotransferase
*NC_0009131,097,1580CT28.6% 40.5 / 18.3 42intergenic (+329/+407)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009132,767,4440CA28.2% 205.8 / 17.5 120intergenic (+89/‑266)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009134,360,9650CA28.0% 323.6 / 23.3 150E324* (GAG→TAG) cadCDNA‑binding transcriptional activator CadC
*NC_0009131,470,6270CA28.0% 322.8 / 24.3 161pseudogene (109/3495 nt)ydbAputative outer membrane protein, N‑terminal fragment

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4606166114 (1.010)10 (0.130) 10/420 5.6 11.6% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 73 (0.920)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 128732976 (0.680)8 (0.100) 7/424 6.9 13.1% intergenic (‑1/+209) tyrV/tyrT tRNA‑Tyr/tRNA‑Tyr
?NC_000913 1287624 = 52 (0.650)intergenic (‑2/+158) tyrT/purU tRNA‑Tyr/formyltetrahydrofolate deformylase
* ? NC_000913 = 281827548 (0.430)6 (0.070) 5/440 8.2 10.9% intergenic (‑1/+198) argY/argV tRNA‑Arg/tRNA‑Arg
?NC_000913 2818551 = 63 (0.760)intergenic (‑2/+2) argV/serV tRNA‑Arg/tRNA‑Ser
* ? NC_000913 = 69674176 (0.680)4 (0.050) 4/442 8.8 7.0% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 50 (0.600)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 432583873 (0.650)4 (0.040) 4/566 10.4 5.8% intergenic (‑97/+561) yjdN/yjdM PF06983 family protein YjdN/zinc ribbon domain‑containing protein YjdM
?NC_000913 4326339 = 60 (0.560)intergenic (‑598/+60) yjdN/yjdM PF06983 family protein YjdN/zinc ribbon domain‑containing protein YjdM
* ? NC_000913 = 128708451 (0.450)4 (0.040) 3/558 11.0 6.6% noncoding (153/171 nt) rttR small RNA RttR
?NC_000913 1287557 = 65 (0.620)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 128690663 (0.560)4 (0.040) 3/558 11.0 6.1% coding (198/276 nt) ychS putative uncharacterized protein YchS
?NC_000913 1287557 = 65 (0.620)noncoding (66/85 nt) tyrT tRNA‑Tyr