breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 47 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,477,2650AC34.7% 194.5 / 46.6 121L122* (TTA→TGA) ‡dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009132,288,4180GT33.6% 172.4 / 24.3 107pseudogene (497/2525 nt)yejOadhesin‑like autotransporter YejO
*NC_0009131,375,7230CA33.6% 165.3 / 13.1 113S254Y (TCC→TAC) ycjQD‑guloside 3‑dehydrogenase
*NC_0009132,767,4450CA32.4% 204.0 / 17.0 109intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009133,911,5330CA31.7% 214.1 / 29.7 142intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009134,136,4670AC31.5% 80.9 / 18.5 54E120D (GAA→GACyijEcystine exporter
*NC_0009131,087,6280AC31.2% 212.5 / 11.1 110D73E (GAT→GAGpgaCpoly‑N‑acetyl‑D‑glucosamine synthase subunit PgaC
*NC_0009133,054,8940GT30.8% 157.9 / 36.5 107P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_00091358,0650CA29.1% 160.4 / 11.8 103P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009131,087,5770AC28.2% 174.3 / 16.4 110V90V (GTT→GTGpgaCpoly‑N‑acetyl‑D‑glucosamine synthase subunit PgaC
*NC_0009131,334,9660AC27.9% 262.4 / 16.4 158intergenic (+137/‑182)cysB/ymiADNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA
*NC_000913141,8490CA27.7% 226.0 / 11.6 119P140Q (CCG→CAG) hpthypoxanthine phosphoribosyltransferase
*NC_000913847,9550GA27.2% 213.7 / 51.6 147A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_0009134,171,5570CA27.1% 156.5 / 60.9 96intergenic (+13/‑80)rrlB/rrfB23S ribosomal RNA/5S ribosomal RNA
*NC_0009131,922,8150CA26.7% 213.4 / 12.5 131G52V (GGA→GTA) pphAphosphoprotein phosphatase 1
*NC_0009132,477,2580GA26.6% 250.6 / 20.3 124D124D (GAC→GATdsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009133,903,0150CA26.6% 122.7 / 23.9 64G229G (GGG→GGTbglB6‑phospho‑beta‑glucosidase B
*NC_0009133,269,9430GT26.3% 120.0 / 11.8 81intergenic (+341/+273)yhaC/rnpBuncharacterized protein YhaC/RNase P catalytic RNA component
*NC_000913147,8020CA26.3% 209.0 / 10.7 118R279R (CGG→AGG) rpnCrecombination‑promoting nuclease RpnC
*NC_0009132,477,2640TC26.1% 248.6 / 13.3 120L122L (TTA→TTG) ‡dsdCDNA‑binding transcriptional dual regulator DsdC

Marginal new junction evidence (lowest skew 10 of 17 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3648146112 (1.020)9 (0.100) 9/482 6.8 9.4% intergenic (+2/‑148) agrA/agrB small RNA AgrA/small regulatory RNA AgrB
?NC_000913 3648378 = 83 (0.930)intergenic (+3/‑150) agrB/arsR small regulatory RNA AgrB/DNA‑binding transcriptional repressor ArsR
* ? NC_000913 = 128708466 (0.600)9 (0.090) 8/558 8.2 12.9% noncoding (153/171 nt) rttR small RNA RttR
?NC_000913 1287557 = 60 (0.580)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 128690672 (0.650)10 (0.100) 8/558 8.2 13.6% coding (198/276 nt) ychS putative uncharacterized protein YchS
?NC_000913 1287557 = 60 (0.580)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 128726283 (0.750)7 (0.070) 7/556 8.6 8.7% noncoding (67/85 nt) tyrV tRNA‑Tyr
?NC_000913 1287382 = 69 (0.670)intergenic (‑54/+156) tyrV/tyrT tRNA‑Tyr/tRNA‑Tyr
* ? NC_000913 1096961 =96 (0.870)4 (0.050) 4/438 8.7 5.7% intergenic (+132/+604) ycdU/serX uncharacterized protein YcdU/tRNA‑Ser
?NC_000913 = 1097503 61 (0.760)intergenic (+674/+62) ycdU/serX uncharacterized protein YcdU/tRNA‑Ser
* ? NC_000913 = 2357664124 (1.130)7 (0.070) 7/576 8.8 6.0% coding (761/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357848 = 100 (0.940)intergenic (+45/+194) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 = 439243782 (0.740)3 (0.040) 3/442 9.4 4.2% intergenic (+2/‑35) glyV/glyX tRNA‑Gly/tRNA‑Gly
?NC_000913 4392661 = 77 (0.950)intergenic (+3/‑231) glyY/yjeV tRNA‑Gly/uncharacterized protein YjeV
* ? NC_000913 = 69683068 (0.620)3 (0.040) 3/444 9.5 5.6% noncoding (1/75 nt) glnW tRNA‑Gln
?NC_000913 696940 = 50 (0.610)intergenic (‑1/+23) glnU/leuW tRNA‑Gln/tRNA‑Leu
* ? NC_000913 = 78132274 (0.670)6 (0.060) 4/520 9.8 8.3% intergenic (+100/‑47) lysY/lysZ tRNA‑Lys/tRNA‑Lys
?NC_000913 781811 = 68 (0.710)intergenic (+159/‑274) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 409435076 (0.690)3 (0.030) 3/516 10.5 4.5% intergenic (‑78/+373) rhaD/rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
?NC_000913 4094719 = 63 (0.660)intergenic (‑447/+4) rhaD/rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase