breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 51 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,037,2750CT64.3% 69.7 / 120.4 113noncoding (16/76 nt)alaTtRNA‑Ala
*NC_0009134,037,4960AT49.7% 55.3 / inf 175intergenic (+161/‑23)alaT/rrlAtRNA‑Ala/23S ribosomal RNA
*NC_0009133,054,8940GT41.1% 103.3 / 37.0 90P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_000913190,8430CA39.4% 115.0 / 12.8 99intergenic (+244/‑14)rpsB/tsf30S ribosomal subunit protein S2/protein chain elongation factor EF‑Ts
*NC_0009132,767,4450CA39.2% 170.3 / 27.7 108intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009131,097,1580CT36.4% 23.9 / 35.3 45intergenic (+329/+407)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009132,767,4440CA36.1% 188.0 / 14.7 108intergenic (+89/‑266)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009132,767,4430CA35.5% 188.6 / 17.6 108intergenic (+88/‑267)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009132,477,2650AC35.2% 148.3 / 33.9 91L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009133,054,8860GT35.2% 130.8 / 31.6 91Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_0009134,296,2680TC35.1% 32.8 / 33.2 38intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009131,611,5070GT34.5% 179.0 / 11.9 116L116L (CTC→CTAdgcFputative diguanylate cyclase DgcF
*NC_0009133,054,8880AC34.4% 145.8 / 12.6 92V186G (GTA→GGA) ygfBPF03695 family protein YgfB
*NC_0009131,631,9030GT31.4% 192.6 / 12.1 105S128Y (TCC→TAC) ydfJputative transporter YdfJ
*NC_0009134,136,4670AC31.4% 111.1 / 20.1 73E120D (GAA→GACyijEcystine exporter
*NC_00091358,0650CA31.4% 187.7 / 14.0 102P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_000913161,5590GA30.9% 125.8 / 12.4 68S158L (TCG→TTG) thpRRNA 2',3'‑cyclic phosphodiesterase
*NC_0009134,249,8100CA30.8% 171.5 / 11.3 91P86Q (CCG→CAG) malMmaltose regulon periplasmic protein
*NC_0009132,895,3490GT30.5% 173.4 / 17.5 82P144Q (CCG→CAG) ygcQputative flavoprotein
*NC_0009132,767,4490CA29.9% 216.6 / 20.3 117intergenic (+94/‑261)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP

Marginal new junction evidence (lowest skew 10 of 23 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3186096 =NA (NA)17 (0.160) 15/598 6.0 13.9% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 3403633 105 (0.960)coding (150/975 nt) yhdH acrylyl‑CoA reductase
* ? NC_000913 2996361 =NA (NA)12 (0.110) 12/598 6.9 10.1% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 3403629 = 107 (0.980)coding (146/975 nt) yhdH acrylyl‑CoA reductase
* ? NC_000913 2818197 =45 (0.410)5 (0.070)
+114 bp
5/370 6.9 15.1% intergenic (‑62/+1) argZ/argY tRNA‑Arg/tRNA‑Arg
?NC_000913 = 2818216 46 (0.420)noncoding (59/77 nt) argY tRNA‑Arg
* ? NC_000913 1186387 =87 (0.800)13 (0.120) 12/596 6.9 13.0% coding (544/1227 nt) pepT peptidase T
?NC_000913 = 1186391 88 (0.810)coding (548/1227 nt) pepT peptidase T
* ? NC_000913 = 69674159 (0.540)6 (0.070) 6/442 7.5 11.5% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 49 (0.610)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 381260 =NA (NA)9 (0.080) 9/596 8.0 5.0% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 572067 170 (1.560)coding (614/798 nt) ybcM putative DNA‑binding transcriptional regulator
* ? NC_000913 = 78122260 (0.550)5 (0.060) 5/446 8.1 8.1% noncoding (76/76 nt) lysY tRNA‑Lys
?NC_000913 781653 = 68 (0.840)intergenic (+1/‑432) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 2996361 =NA (NA)8 (0.070) 8/598 8.4 8.8% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 3396891 = 83 (0.760)coding (905/1470 nt) rng RNase G
* ? NC_000913 = 382590NA (NA)9 (0.080) 8/598 8.4 5.0% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 572063 = 170 (1.560)coding (610/798 nt) ybcM putative DNA‑binding transcriptional regulator
* ? NC_000913 = 128708468 (0.620)7 (0.070) 7/558 8.5 8.9% noncoding (153/171 nt) rttR small RNA RttR
?NC_000913 1287557 = 80 (0.790)noncoding (66/85 nt) tyrT tRNA‑Tyr