breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 45 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,208,1320GA71.2% 209.9 / 154.5 179intergenic (‑600/‑15)purH/rrsEbifunctional AICAR transformylase/IMP cyclohydrolase/16S ribosomal RNA
*NC_0009133,943,4520GA50.0% ‑3.9 / 83.7 50noncoding (18/76 nt)gltUtRNA‑Glu
*NC_0009131,097,1180CA46.7% 10.5 / 51.4 45intergenic (+289/+447)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009132,477,2650AC40.8% 133.2 / 33.9 98L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009133,943,6340AG40.4% 101.1 / 185.0 149intergenic (+124/‑70)gltU/rrlCtRNA‑Glu/23S ribosomal RNA
*NC_0009133,054,8860GT38.3% 153.9 / 39.8 108Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_00091358,0650CA35.0% 159.9 / 18.6 97P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009131,334,9660AC34.4% 224.6 / 36.7 163intergenic (+137/‑182)cysB/ymiADNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA
*NC_0009131,097,1580CT34.2% 29.3 / 29.8 38intergenic (+329/+407)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009133,054,8940GT30.4% 150.1 / 29.9 103P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_0009133,911,5330CA30.1% 284.0 / 22.7 153intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009131,502,7720GA29.8% 146.3 / 28.0 94A106T (GCG→ACG) ydcLDUF3313 domain‑containing lipoprotein YdcL
*NC_0009131,502,7700TC29.5% 156.9 / 28.7 95I105T (ATA→ACA) ydcLDUF3313 domain‑containing lipoprotein YdcL
*NC_0009133,741,8710GT28.9% 252.7 / 28.4 135S221Y (TCC→TAC) plaRDNA‑binding transcriptional repressor PlaR
*NC_0009134,209,7910GA28.6% 310.3 / 235.4 252noncoding (18/76 nt)gltVtRNA‑Glu
*NC_0009132,767,4490CA27.8% 296.6 / 12.6 133intergenic (+94/‑261)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009131,922,8150CA27.4% 170.1 / 29.2 106G52V (GGA→GTA) pphAphosphoprotein phosphatase 1
*NC_000913161,5590GA27.0% 152.8 / 12.3 74S158L (TCG→TTG) thpRRNA 2',3'‑cyclic phosphodiesterase
*NC_0009134,360,9650CA26.9% 364.3 / 31.1 175E324* (GAG→TAG) cadCDNA‑binding transcriptional activator CadC
*NC_000913847,9550GA26.5% 215.5 / 35.6 123A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 460616693 (0.900)8 (0.110) 8/420 5.2 10.0% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 79 (1.090)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 2357730122 (1.190)7 (0.080) 7/518 6.6 6.4% coding (827/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357926 = 98 (1.100)intergenic (+123/+116) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 = 128732972 (0.700)4 (0.050) 4/424 7.0 8.7% intergenic (‑1/+209) tyrV/tyrT tRNA‑Tyr/tRNA‑Tyr
?NC_000913 1287624 = 33 (0.450)intergenic (‑2/+158) tyrT/purU tRNA‑Tyr/formyltetrahydrofolate deformylase
* ? NC_000913 = 69674180 (0.780)4 (0.050) 4/442 7.2 6.1% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 64 (0.840)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 128690654 (0.530)8 (0.080) 5/558 7.9 13.9% coding (198/276 nt) ychS putative uncharacterized protein YchS
?NC_000913 1287557 = 49 (0.510)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 230450048 (0.470)3 (0.030) 3/506 8.5 6.4% intergenic (+107/+608) eco/mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase
?NC_000913 2305066 = 47 (0.540)intergenic (+673/+42) eco/mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase
* ? NC_000913 = 287803473 (0.710)4 (0.040) 3/540 8.9 5.2% intergenic (+416/+535) iap/cas2 alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
?NC_000913 2878281 = 80 (0.860)intergenic (+663/+288) iap/cas2 alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
* ? NC_000913 = 265352099 (0.960)4 (0.040)
+TTATTATCGACAC
3/572 9.2 4.1% intergenic (‑65/‑335) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
?NC_000913 2653856 = 96 (0.930)noncoding (2/304 nt) ryfA small regulatory RNA RyfA
* ? NC_000913 = 696433118 (1.150)3 (0.030)
+C
3/596 9.4 2.8% noncoding (72/75 nt) glnX tRNA‑Gln
?NC_000913 696870 = 91 (0.880)noncoding (70/75 nt) glnU tRNA‑Gln