breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 163 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913581,3350CA79.8% 917.3 / 268.8 453L168M (CTG→ATG) ‡nohDputative DNA‑packaging protein NohD
*NC_000913566,6510TC79.6% 1062.6 / 261.3 466intergenic (‑673/‑33)intD/renDputative integrase/protein RenD
*NC_000913566,5650AG79.5% 831.6 / 206.1 381intergenic (‑587/‑119)intD/renDputative integrase/protein RenD
*NC_000913566,5700AC79.4% 947.6 / 202.7 389intergenic (‑592/‑114)intD/renDputative integrase/protein RenD
*NC_000913581,3810AG79.3% 1000.0 / 268.8 454intergenic (+2/‑153)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,3800CG78.7% 1009.7 / 302.9 455intergenic (+1/‑154)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_0009131,951,3860CT77.5% 584.9 / 150.2 276L185L (CTC→CTTyecDisochorismatase family protein YecD
*NC_0009131,951,4120AG76.3% 505.1 / 163.9 254L6L (CTA→CTGyecEDUF72 domain‑containing protein YecE
*NC_000913581,4160AG75.8% 687.0 / 251.5 364intergenic (+37/‑118)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,2750GA75.8% 697.3 / 223.3 334V148I (GTT→ATT) nohDputative DNA‑packaging protein NohD
*NC_000913581,3940TC75.5% 669.3 / 267.2 367intergenic (+15/‑140)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,3980CG75.4% 762.1 / 290.4 366intergenic (+19/‑136)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,3970TC75.3% 676.9 / 261.4 365intergenic (+18/‑137)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,4120GT75.3% 633.5 / 267.4 366intergenic (+33/‑122)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,2900CA75.3% 572.1 / 244.2 336R153R (CGG→AGG) ‡nohDputative DNA‑packaging protein NohD
*NC_000913581,2910GA75.3% 590.4 / 215.1 337R153Q (CGG→CAG) ‡nohDputative DNA‑packaging protein NohD
*NC_000913581,3010CT75.3% 660.6 / 236.0 336I156I (ATC→ATTnohDputative DNA‑packaging protein NohD
*NC_000913581,3920TC75.2% 686.3 / 264.5 367intergenic (+13/‑142)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,4130CT75.2% 735.6 / 258.1 367intergenic (+34/‑121)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD
*NC_000913581,4280GT75.2% 655.7 / 271.0 367intergenic (+49/‑106)nohD/tfaDputative DNA‑packaging protein NohD/putative tail fiber assembly protein TfaD

Marginal new junction evidence (lowest skew 10 of 20 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 696741156 (1.570)24 (0.330) 17/442 2.3 16.9% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 120 (1.640)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 780629167 (1.680)15 (0.200) 13/446 3.1 12.4% noncoding (76/76 nt) lysT tRNA‑Lys
?NC_000913 780919 = 87 (1.180)intergenic (+1/‑149) lysW/valZ tRNA‑Lys/tRNA‑Val
* ? NC_000913 = 460616693 (0.940)10 (0.140) 10/420 3.5 14.0% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 58 (0.830)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 4542682 =142 (1.430)15 (0.160) 13/580 4.0 10.0% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 131 (1.360)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 696504167 (1.680)11 (0.120) 10/540 4.4 6.6% noncoding (1/75 nt) glnX tRNA‑Gln
?NC_000913 696940 = 161 (1.800)intergenic (‑1/+23) glnU/leuW tRNA‑Gln/tRNA‑Leu
* ? NC_000913 = 4542690164 (1.650)11 (0.110) 10/580 4.7 7.0% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 131 (1.360)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 409435077 (0.780)6 (0.070) 6/516 5.5 9.5% intergenic (‑78/+373) rhaD/rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
?NC_000913 4094719 = 48 (0.560)intergenic (‑447/+4) rhaD/rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
* ? NC_000913 = 265341976 (0.770)8 (0.090) 6/548 5.7 10.0% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 75 (0.820)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 781577 =136 (1.370)7 (0.070)
+GAAG
7/590 5.7 6.4% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 70 (0.710)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 34969336 (0.360)5 (0.050) 5/564 6.3 9.0% intergenic (+121/‑319) prpB/prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
?NC_000913 349973 = 67 (0.720)intergenic (+401/‑39) prpB/prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase