Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 3,731,420 IS1 (–) +9 bp coding (290‑298/993 nt) xylF → xylose ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3583428 =NA (NA)36 (1.240) 34/484 0.1 89.6% noncoding (1/768 nt) IS1 repeat region
?NC_000913 3731420 = 4 (0.140)coding (290/993 nt) xylF xylose ABC transporter periplasmic binding protein
* ? NC_000913 = 3584195NA (NA)53 (1.830) 49/484 0.0 92.7% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 3731428 4 (0.140)coding (298/993 nt) xylF xylose ABC transporter periplasmic binding protein

CCCTTTGACGTGGTGATATGGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGTGATTTCTCTTCATTCTTAAACGAAATCATGGACAGTTGTTCATAATAATCAAAAGGAAACAAAAATCATTGTAAACCATGCCATCTTTTTAGAGATTACCAGCAGAAAATTCCTTGTTCGAAATTTAGCAGTGCAAGCTATGGAAAAACTTACAACGGAACTACACTCCTTA  >  NC_000913/3583950‑3584436
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 
cccTTAGACGTGGTGATATGGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCGGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACa                                                                                                                                                                                                                                               <  1:331159/250‑1 (MQ=14)
       aCGTGGTGATATGGATGGCGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGt                                                                                                                                                                                                                                       <  1:159445/251‑1 (MQ=21)
           ggtgATATGGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATGACCGACATTAAAAGTAAc                                                                                                                                                                                                                                    <  1:98077/250‑1 (MQ=37)
              gATATGGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTTGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGAc                                                                                                                                                                                                                                                >  1:81136/1‑235 (MQ=14)
              gATATGGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGGCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTACCtttt                                                                                                                                                                                                                                >  2:273067/1‑251 (MQ=25)
              gATAGGGATGACGGAAGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTTGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGAc                                                                                                                                                                                                                                                <  2:81136/235‑1 (MQ=9)
                 agggagtaCGGAGGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTg                                                                                                                                                                                                                             <  2:121137/244‑1 (MQ=255)
                           gagggctggcAGCTGTAGGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGAGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCAGGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAgaga                                                                                                                                                                                                                   <  2:123145/248‑1 (MQ=255)
                                  ggccgctttagaaaTCCCGCCTGAAGGGAAAGCTGTACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATAtt                                                                                                                                                                                                            <  2:315155/239‑1 (MQ=255)
                                                      cTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATATGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTc                                                                                                                                                                                          >  1:165402/1‑249 (MQ=255)
                                                                 ccTGCACGTAATCAGCAAGCGATATACGCCGCGAATTGAGCGGCATAACCAGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGCCAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTACCTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTAAGTTCGTTTAGTg                                                                                                                                                                             >  2:42365/2‑251 (MQ=255)
                                                                        gTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGGGATTtctc                                                                                                                                                                      >  2:5983/1‑251 (MQ=255)
                                                                             cAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGACATCGGGCATTATCTGAACATAAAACACTATCAATAAGGTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGTAATTAGAGATACTATTGGCACAGAAAATATATTCAGTTCGTTTAGGGATTTCTCTTCAt                                                                                                                                                                 >  2:272996/1‑251 (MQ=255)
                                                                                        ataCGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGTGATTTCTCTTCATTCTTAAACGa                                                                                                                                                       >  1:10710/1‑250 (MQ=255)
                                                                                                cGAATTGAACGGCAAAACATGTA‑TCTGAGGCAGCTCCTGGCACGGTTGGGACGGAATACGCGGTCGTTCTAAAAATCGGTTGATCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTGTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGTGATTTCTCTTCATTCTTAAACGAAATCATGGa                                                                                                                                              <  2:181885/251‑1 (MQ=21)
                                                                                                  aaTTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTTATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGTGATTTCTCTTCATTCTTAAACGAAATCATGGACa                                                                                                                                            <  1:241699/251‑1 (MQ=255)
                                                                                                        gcgggctAACCTGAAGTC‑GAGGCAGCACCCGGCACGTCTGGGACGGAAGTCGTTGTCGATCACAAATTCGGTGGAGCAGCATGACAAAGTCATCTGGAATTATCTGAAAAAAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGCAAATATATTCAGTTCGTTTAGTGATTTCTCTTCATTCTTAAACGAAATCATGGCCAgttgtt                                                                                                                                      <  2:110424/245‑1 (MQ=21)
                                                                                                                                       ggCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGTGATTTCTCTTCATTCTTAAACGAAATCATGGACAGTTGTTCATAATAATCAAAAGGAAACAAAAATCAt                                                                                                         <  1:5983/250‑1 (MQ=255)
                                                                                                                                          ccGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGTGATTTCTCTTCATTCTTAAACGAAATCATGGACAGTTGTTCATAATAATCAAAAGGAAACAAAAATCATTGTa                                                                                                     <  1:273067/250‑1 (MQ=255)
                                                                                                                                                                                      tGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGTGATTTCTCTTCATTCTTAAACGAAATCATGGACAGTTGTTCATAATAATCAAAAGGAAACAAAAATCATTGTAAACCATGCCATCTTTTTAGAGATTACCAGCAGAAAATTCCTTGt                                                         >  1:87848/1‑251 (MQ=255)
                                                                                                                                                                                                                                               tCATTACCGCCATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGTGATTTCTCTTCATTCTTAAACGAAATCATGGACAGTTGTTCATAATAATCAAAAGGAAACAAAAATAATTGTAAACCATGCCATCTTTTTAGAGATTACCAGCAGAAAATTCCTTGTTCGAAATTTAGCAGTGCAAGCTATGGAAAAACTTACAACGAAACTACACTCCTTa  >  2:105787/1‑249 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 
CCCTTTGACGTGGTGATATGGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAA‑TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGACATTAAAAGTAACTTTTTTGAAATTAGAGATAATATTGGCACAGAAAATATATTCAGTTCGTTTAGTGATTTCTCTTCATTCTTAAACGAAATCATGGACAGTTGTTCATAATAATCAAAAGGAAACAAAAATCATTGTAAACCATGCCATCTTTTTAGAGATTACCAGCAGAAAATTCCTTGTTCGAAATTTAGCAGTGCAAGCTATGGAAAAACTTACAACGGAACTACACTCCTTA  >  NC_000913/3583950‑3584436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.