breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 44 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,509,5860AC50.0% 18.2 / 207.0 176pseudogene (108/315 nt)insOIS911B regulator fragment
*NC_0009134,509,4870AG44.0% 52.9 / 213.2 168pseudogene (9/315 nt)insOIS911B regulator fragment
*NC_0009134,509,4160TC43.4% 67.0 / 210.3 173pseudogene (408/471 nt)insOIS911B regulator fragment
*NC_0009134,509,4280AG43.2% 74.5 / 196.4 169pseudogene (420/471 nt)insOIS911B regulator fragment
*NC_0009134,509,5440GA43.1% 87.2 / 205.7 175pseudogene (66/315 nt)insOIS911B regulator fragment
*NC_0009134,509,5370GA42.9% 72.5 / 204.6 175pseudogene (59/315 nt)insOIS911B regulator fragment
*NC_0009134,037,4960AT42.4% 109.7 / 234.7 151intergenic (+161/‑23)alaT/rrlAtRNA‑Ala/23S ribosomal RNA
*NC_0009134,509,4630TG42.2% 61.9 / 225.1 174pseudogene (455/471 nt)insOIS911B regulator fragment
*NC_0009134,509,5240CT42.1% 91.9 / 205.6 178pseudogene (46/315 nt)insOIS911B regulator fragment
*NC_0009134,509,5560TC41.2% 87.9 / 195.1 170pseudogene (78/315 nt)insOIS911B regulator fragment
*NC_0009134,509,5620TC41.0% 86.4 / 195.3 173pseudogene (84/315 nt)insOIS911B regulator fragment
*NC_0009134,509,4781.C40.9% 110.6 / inf 171pseudogene (470/471 nt)insOIS911B regulator fragment
*NC_0009134,509,5920GA38.4% 139.7 / 174.9 172pseudogene (114/315 nt)insOIS911B regulator fragment
*NC_0009132,246,7790CA36.3% 130.8 / 12.8 102intergenic (‑10/+284)cirA/lysPiron‑catecholate outer membrane transporter CirA/lysine:H(+) symporter
*NC_0009134,509,6230GA35.6% 164.7 / 147.1 160pseudogene (145/315 nt)insOIS911B regulator fragment
*NC_0009132,990,4800CA33.1% 186.8 / 16.5 124intergenic (‑120/‑788)yqeK/ygeGprotein YqeK/TPR repeat‑containing putative chaperone YgeG
*NC_0009134,360,9650CA32.6% 254.4 / 24.6 144E324* (GAG→TAG) cadCDNA‑binding transcriptional activator CadC
*NC_0009133,054,8940GT31.8% 101.0 / 13.4 66P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_0009134,509,6280AG31.3% 186.9 / 123.4 150pseudogene (150/315 nt)insOIS911B regulator fragment
*NC_0009134,509,6300CT31.1% 203.6 / 115.9 151pseudogene (152/315 nt)insOIS911B regulator fragment

Marginal new junction evidence (lowest skew 10 of 28 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 409435071 (0.830)11 (0.150) 11/508 3.9 16.7% intergenic (‑78/+373) rhaD/rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
?NC_000913 4094719 = 49 (0.670)intergenic (‑447/+4) rhaD/rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
* ? NC_000913 = 460616685 (0.990)7 (0.120) 7/412 4.2 11.0% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 54 (0.900)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 69646956 (0.650)18 (0.210)
+TAGAAT
12/578 4.2 26.3% noncoding (36/75 nt) glnX tRNA‑Gln
?NC_000913 696802 = 47 (0.550)noncoding (29/75 nt) glnW tRNA‑Gln
* ? NC_000913 = 294746765 (0.760)7 (0.110) 7/424 4.4 12.7% intergenic (+4/‑30) metZ/metW tRNA‑Met/tRNA‑Met
?NC_000913 2947688 = 50 (0.810)intergenic (+5/+69) metV/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
* ? NC_000913 = 128690648 (0.560)14 (0.180) 9/550 4.8 25.1% coding (198/276 nt) ychS putative uncharacterized protein YchS
?NC_000913 1287557 = 39 (0.490)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 78122256 (0.650)8 (0.130) 6/438 4.9 16.9% noncoding (76/76 nt) lysY tRNA‑Lys
?NC_000913 781445 = 37 (0.580)intergenic (+1/‑132) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
* ? NC_000913 = 281827548 (0.560)5 (0.080) 5/432 5.2 11.3% intergenic (‑1/+198) argY/argV tRNA‑Arg/tRNA‑Arg
?NC_000913 2818551 = 43 (0.690)intergenic (‑2/+2) argV/serV tRNA‑Arg/tRNA‑Ser
* ? NC_000913 = 265341971 (0.830)10 (0.130) 7/540 5.4 13.6% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 62 (0.790)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 781369 =45 (0.530)10 (0.120)
+GG
8/586 5.4 14.8% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 71 (0.830)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 128708459 (0.690)7 (0.090) 7/550 5.5 13.0% noncoding (153/171 nt) rttR small RNA RttR
?NC_000913 1287557 = 39 (0.490)noncoding (66/85 nt) tyrT tRNA‑Tyr