breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 50 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,661,3940GA66.7% 96.2 / 74.3 78G280D (GGC→GAC) ynfFputative selenate reductase YnfF
*NC_0009134,166,8490GA45.3% 20.5 / 72.0 53noncoding (191/1542 nt)rrsB16S ribosomal RNA
*NC_000913190,8430CA36.3% 172.6 / 10.2 124intergenic (+244/‑14)rpsB/tsf30S ribosomal subunit protein S2/protein chain elongation factor EF‑Ts
*NC_0009132,472,8170CA35.7% 141.0 / 16.4 98G22V (GGA→GTA) yfdNuncharacterized protein YfdN
*NC_0009133,269,9810AC34.8% 174.9 / 19.1 117intergenic (+379/+235)yhaC/rnpBuncharacterized protein YhaC/RNase P catalytic RNA component
*NC_0009133,911,5330CA34.1% 293.1 / 24.4 174intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009134,425,4180CA32.7% 165.2 / 10.4 101P101T (CCG→ACG) rpsF30S ribosomal subunit protein S6
*NC_0009134,427,8220CA32.2% 261.7 / 13.5 149intergenic (‑400/+257)yjfZ/ytfBDUF2686 domain‑containing protein YjfZ/cell division protein YtfB
*NC_0009132,767,4450CA32.0% 231.9 / 16.3 103intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_000913847,9550GA31.6% 196.6 / 61.4 139A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_0009131,557,4280GT30.5% 121.1 / 10.4 82S204* (TCG→TAG) ddpFputative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpF
*NC_0009131,390,6070CA29.5% 253.7 / 14.6 149intergenic (‑9/+73)mpaA/ymjCmurein tripeptide amidase A/putative uncharacterized protein YmjC
*NC_00091358,0650CA29.5% 176.2 / 21.9 105P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009133,054,8860GT29.4% 194.5 / 31.5 119Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_000913442,0250CA29.2% 184.9 / 11.2 106L159L (CTC→CTAthiItRNA uridine 4‑sulfurtransferase
*NC_0009133,054,8940GT29.2% 173.5 / 21.6 106P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_000913141,8490CA28.2% 274.2 / 25.5 143P140Q (CCG→CAG) hpthypoxanthine phosphoribosyltransferase
*NC_000913228,6890T.27.7% 217.3 / 130.7 114intergenic (+27/‑67)rrlH/rrfH23S ribosomal RNA/5S ribosomal RNA
*NC_0009133,032,3070CT27.6% 208.4 / 31.3 116T314M (ACG→ATG) uacTurate:H(+) symporter
*NC_0009134,261,0400CA27.2% 367.3 / 20.5 184Q148K (CAA→AAA) yjbMDUF2713 domain‑containing protein YjbM

Marginal new junction evidence (lowest skew 10 of 34 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4606166104 (0.930)17 (0.220) 16/410 3.2 20.9% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 56 (0.720)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 3648146109 (0.980)15 (0.170) 13/472 4.6 14.4% intergenic (+2/‑148) agrA/agrB small RNA AgrA/small regulatory RNA AgrB
?NC_000913 3648378 = 91 (1.020)intergenic (+3/‑150) agrB/arsR small regulatory RNA AgrB/DNA‑binding transcriptional repressor ArsR
* ? NC_000913 = 2521006111 (1.000)11 (0.130) 9/436 5.5 14.0% noncoding (76/76 nt) valU tRNA‑Val
?NC_000913 2521249 = 53 (0.640)intergenic (+1/‑4) valY/lysV tRNA‑Val/tRNA‑Lys
* ? NC_000913 = 2653419115 (1.030)15 (0.150) 12/538 5.6 12.1% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 112 (1.100)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 = 128732966 (0.590)9 (0.110) 8/414 5.6 15.7% intergenic (‑1/+209) tyrV/tyrT tRNA‑Tyr/tRNA‑Tyr
?NC_000913 1287624 = 50 (0.640)intergenic (‑2/+158) tyrT/purU tRNA‑Tyr/formyltetrahydrofolate deformylase
* ? NC_000913 = 128708465 (0.580)12 (0.120) 11/548 6.0 16.7% noncoding (153/171 nt) rttR small RNA RttR
?NC_000913 1287557 = 59 (0.570)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 294746762 (0.560)8 (0.100) 7/422 6.1 13.5% intergenic (+4/‑30) metZ/metW tRNA‑Met/tRNA‑Met
?NC_000913 2947688 = 58 (0.720)intergenic (+5/+69) metV/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
* ? NC_000913 = 2817942115 (1.030)11 (0.110) 10/548 6.4 10.5% intergenic (‑82/+117) argQ/argZ tRNA‑Arg/tRNA‑Arg
?NC_000913 2818492 = 81 (0.780)noncoding (58/77 nt) argV tRNA‑Arg
* ? NC_000913 = 439243786 (0.770)6 (0.070) 6/432 6.7 7.6% intergenic (+2/‑35) glyV/glyX tRNA‑Gly/tRNA‑Gly
?NC_000913 4392661 = 83 (1.010)intergenic (+3/‑231) glyY/yjeV tRNA‑Gly/uncharacterized protein YjeV
* ? NC_000913 = 2653549114 (1.020)8 (0.080) 8/556 7.2 7.1% intergenic (‑94/‑306) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
?NC_000913 2653872 = 103 (0.980)noncoding (18/304 nt) ryfA small regulatory RNA RyfA