Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_012971	2,969,387	Δ670 bp	coding (226‑895/1494 nt)	gspE ←	type II secretion system ATPase GspE

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_012971	2969387	2970056	670	59 [0]	[0] 59	gspE	type II secretion system ATPase GspE

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_012971	= 2969386	0 (0.000)	59 (0.620)	39/586	1.1	100%	coding (896/1494 nt)	gspE	type II secretion system ATPase GspE
	?	NC_012971	2970057 =	0 (0.000)					coding (225/1494 nt)	gspE	type II secretion system ATPase GspE

TTGATACGCGAGACCAGCAGCGAGGCCAGCTTGCGACCGGGGCGCAACATTTCATGTAATGTGCCGTCAACACGAAAACGGATCACCAGACTCTTTTCAAACGTCTCGATGTGGATATCCGAAGCGCCTTCTTTAATCGCCTCTGCCAGCATGGCGTTGATCAGTTTGATGATCGGCGCATCGTCGTCACTTTCCAGCAGATCTTCCGTTTCCGGCAGTTCTTCTGCGAGGGTAAAAAAGTCCTCGGCAGAACCGAGATCTTCCATCAGTTGGCGAGCCTCGGAAGAGTCCCGCTGCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAGGAATGCGCGAACTTCCGGCGGTAAGGCCTGGTTTTGCGCTGGCAGTACC  >  NC_012971/2969761‑2970353                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                         ggcctggcggcagtcggggcacagcgtacggaccagtcgctgcgccatcacgcccgtcagactggaagagagcaggaaaggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCa                                                                                                                                                                                                                                                                                                        >  2:690553‑M2/297‑299 (MQ=255)     tggcggcagtcggggnacagcgtacggaccagtcnctgcgccatcacgcccgtcagactggaagagagcaggaaaggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcattacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtnttaacctgtgCCAGAc                                                                                                                                                                                                                                                                                                     <  1:682975‑M2/6‑1 (MQ=255)     tggcggcagtcggggnacagcgtacggaccagtcnctgcgccatcacgcccgtcagactggaagagagcaggaaaggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtnttaacctgtgCCAGAc                                                                                                                                                                                                                                                                                                     <  1:682666‑M2/6‑1 (MQ=255)     tggcggcagtcggggcacagcgtacggaccagtcgctgcgccatcacgcccgtcagactggaagagagcaggaaaggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGAc                                                                                                                                                                                                                                                                                                     <  1:781757‑M2/6‑1 (MQ=255)          gcagtcggggcacagcgtacggaccagtcgctgcgccatcacgcccgtcagactggaagagagcaggaaaggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTc                                                                                                                                                                                                                                                                                              <  1:455352‑M2/13‑1 (MQ=255)                ggggcacagcgtacggaccagtcgctgcgccatcacgcccgtcagactggaagagagcaggaaaggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGc                                                                                                                                                                                                                                                                                        <  1:757851‑M2/19‑1 (MQ=255)                        gcgtacggaccagtcgctgcgccatcacgcccgtcagactggaagagagcaggaaaggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTtcaca                                                                                                                                                                                                                                                                                >  1:472738‑M2/274‑298 (MQ=255)                        gcgtacggaccagtcgctgcgccatcacgcccgtcagactggaagagagcaggaaaggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCaaa                                                                                                                                                                                                                                                                                >  2:691815‑M2/274‑300 (MQ=255)                           tacggaccagtcgctgcgccatcacgcccgtcagactggaagagagcagtaaaggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcgggatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAAtt                                                                                                                                                                                                                                                                              <  2:196896‑M2/29‑1 (MQ=255)                                     tcgctgcgccatcacgcccgtcagactggaagagagcaggaaaggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaancgcgcgcaggcaacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTtcgtc                                                                                                                                                                                                                                                                    >  2:630598‑M2/261‑299 (MQ=255)                                                         tcagactggaagagagcaggaaaggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAggg                                                                                                                                                                                                                                               <  1:761038‑M2/60‑1 (MQ=255)                                                            gactggaagagagcaggaaaggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcaatacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGgcgc                                                                                                                                                                                                                                            >  2:754834‑M2/238‑300 (MQ=255)                                                                                ggctctacccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccaagggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCt                                                                                                                                                                                                                        >  1:36959‑M2/218‑300 (MQ=255)                                                                                       cccccatatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCaa                                                                                                                                                                                                                 >  1:186335‑M2/211‑300 (MQ=255)                                                                                         cccatatcctgcaaacncgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCGGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTANAGGGCGCGTTCAGCCCCCNCTGCAGCTCCTGCaaaa                                                                                                                                                                                                               >  1:687394‑M2/209‑300 (MQ=255)                                                                                            atatcctgcaaacgcgtaatcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAAc                                                                                                                                                                                                              <  1:462265‑M2/93‑1 (MQ=255)                                                                                                               tcgcccccaccgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAAc                                                                                                                                                                                         <  1:188012‑M2/114‑1 (MQ=255)                                                                                                                       accgccgtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATggag                                                                                                                                                                                   <  1:642485‑M2/120‑1 (MQ=255)                                                                                                                             gtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCNGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCa                                                                                                                                                                           >  1:151998‑M2/173‑300 (MQ=255)                                                                                                                             gtgttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCa                                                                                                                                                                           >  1:293962‑M2/173‑300 (MQ=255)                                                                                                                                ttggtatgcagcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGNTTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGc                                                                                                                                                                         >  2:623889‑M2/170‑299 (MQ=255)                                                                                                                                          gcgtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCa                                                                                                                                                              >  1:137009‑M2/160‑300 (MQ=255)                                                                                                                                          gcgtggaaaggaccaagtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgaggatttcaccgaccatcaatacatccgggtcctgacgcaaaatcgcgcgcaggccaagggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATCCGGCTTCGTCGCTTTGCCGCAGCGTAAAGGGCGCGTTCCGCCCACGCTGCAGCTCCTGCAAAACAGAGAGCGAAAACAGATGAACACGGAGGAGCTCCAGTGCCGCTCCGCa                                                                                                                                                              >  2:384005‑M2/160‑300 (MQ=255)                                                                                                                                            gtggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCAc                                                                                                                                                             <  2:293962‑M2/142‑1 (MQ=255)                                                                                                                                            gtggaaaggaccaggtgtcaggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCAc                                                                                                                                                             <  1:612160‑M2/142‑1 (MQ=255)                                                                                                                                             tggaaaggaccaggtgtccggtaattgaagcctgtacggcggtttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCAc                                                                                                                                                             <  2:151998‑M2/142‑1 (MQ=255)                                                                                                                                              ggaaaggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCAt                                                                                                                                                          <  1:289597‑M2/145‑1 (MQ=255)                                                                                                                                                   ggaccaggtgtccggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGccac                                                                                                                                                     <  1:775937‑M2/150‑1 (MQ=255)                                                                                                                                                                ggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAAcc                                                                                                                                          <  1:101872‑M2/161‑1 (MQ=255)                                                                                                                                                                ggtaagtgaagcctggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtccngacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAAcc                                                                                                                                          <  1:157925‑M2/161‑1 (MQ=255)                                                                                                                                                                             tggacggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtactgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCAGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGt                                                                                                                           <  1:114704‑M2/176‑1 (MQ=255)                                                                                                                                                                                  ggcgatttctgcggtttcggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGc                                                                                                                      <  2:556639‑M2/181‑1 (MQ=255)                                                                                                                                                                                                    ggtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGc                                                                                                     <  1:522954‑M2/198‑1 (MQ=255)                                                                                                                                                                                                     gtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAg                                                                                                   <  2:806190‑M2/200‑1 (MQ=255)                                                                                                                                                                                                     gtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAg                                                                                                   <  1:718467‑M2/200‑1 (MQ=255)                                                                                                                                                                                                     gtatcgcggatttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTGAGCAg                                                                                                   <  1:718457‑M2/200‑1 (MQ=255)                                                                                                                                                                                                               tttcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCtg                                                                                         <  1:454436‑M2/210‑1 (MQ=255)                                                                                                                                                                                                                 tcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTc                                                                                            >  1:723762‑M2/89‑295 (MQ=255)                                                                                                                                                                                                                 tcaccgaccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGAACGGTGTTAGAAGTGGTTTc                                                                                            >  2:237296‑M2/89‑295 (MQ=255)                                                                                                                                                                                                                       accatcactacatccggttcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgcctacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAg                                                                                 <  1:272241‑M2/218‑1 (MQ=255)                                                                                                                                                                                                                       accatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAg                                                                                 <  1:272001‑M2/218‑1 (MQ=255)                                                                                                                                                                                                                       accatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAg                                                                                 <  2:675177‑M2/218‑1 (MQ=255)                                                                                                                                                                                                                        ccatcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACa                                                                                  >  1:540603‑M2/82‑298 (MQ=255)                                                                                                                                                                                                                          atcactacatccgggtcctgacgcaaaatcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCa                                                                              <  2:691313‑M2/221‑1 (MQ=255)                                                                                                                                                                                                                          atcactacatccggggcctgacgcaaaatcgcgcgcaggccacgggcaaaggtcatgccgacacgggtgttaaccggtgCCAGACCGCATTCAGCCGCTGTTCAAATTTGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCATCGACGCTTCGCACCATGCCACCATGCTAAACCTACGGCTGAAACTGTAGGACAAACGCACGGTGTCAGCActggttccctatcctcccc                                                                                 <  2:280514‑M2/218‑10 (MQ=255)                                                                                                                                                                                                                                                      tcgcgcgcaggccacgggcgaaggtcatgcctacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAgg                                                  <  2:313199‑M2/249‑1 (MQ=255)                                                                                                                                                                                                                                                      tcgcgcgcaggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCTGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAACTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACTGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAgg                                                  <  2:313476‑M2/249‑1 (MQ=255)                                                                                                                                                                                                                                                        gcgcgcaggccacggtcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTTAGGaa                                                <  2:387020‑M2/251‑1 (MQ=255)                                                                                                                                                                                                                                                               ggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCTCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAGGAATGCGCGa                                         >  1:402439‑M2/43‑300 (MQ=255)                                                                                                                                                                                                                                                               ggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAGGAATGCGCGa                                         >  2:542509‑M2/43‑300 (MQ=255)                                                                                                                                                                                                                                                               ggccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAGGAATGCGCGa                                         >  1:382282‑M2/43‑300 (MQ=255)                                                                                                                                                                                                                                                                 ccacgggcgaaggtcatgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAGGAATGCGCGaa                                        >  2:545807‑M2/41‑299 (MQ=255)                                                                                                                                                                                                                                                                                 tgccgacgcgggtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAGGAATGCGCGAACTTCCGGCGGTAAGGcc                       <  1:530596‑M2/276‑1 (MQ=255)                                                                                                                                                                                                                                                                                            gtgttaacctgtgCCANACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAGGANTGCGCGAACTTCCGGCGGNAAGGCCTGGTTTTgcgc            >  1:673343‑M2/14‑300 (MQ=255)                                                                                                                                                                                                                                                                                            gtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCCCCATTAACGCGTTCTCCCGGCATTGAGGAATGCGCGAACTTCCGGCGGTAAGGCCTGGTTTTgcgc            >  2:441171‑M2/14‑300 (MQ=255)                                                                                                                                                                                                                                                                                            gtgttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAGGAATGCGCGAACTTCCGGCGGTAAGGCCTGGTTTTgcgc            >  1:13248‑M2/14‑300 (MQ=255)                                                                                                                                                                                                                                                                                              gttaacctgtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCAACAGGCTAAACCGACGGCTGAAACTGTAGGGCAGAAGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAGGAATGCGCGAACTTCCGGCGGTAAGGACTGGTTTTGCGCt           >  2:169458‑M2/12‑299 (MQ=255)                                                                                                                                                                                                                                                                                                      gtgCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAGGAATGCGCGAACTTCCGGCGGTAAGGCCTGGTTTTGCGCTGGCAGTAcc  >  1:741173‑M2/4‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      gtgCCAAACCGCATCCAGCCGCTGTTCAAATTCGGCTCCGTCGATTTGCCGCAGCGTAAAGGACACGCTCAGCACCCGCTGCAGCTCCTGCAAGACAAAGAGCGACAACGGATGAACATGAAGAATCTACAGCGACGCTCAGCACCATACCACCAGGCTAAACCGACGACTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGTACCATTACCGCGTTCTCCCGGCATTGAGGAATACGCGAACTTcc                                   >  2:335069‑M2/4‑267 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                         TTGATACGCGAGACCAGCAGCGAGGCCAGCTTGCGACCGGGGCGCAACATTTCATGTAATGTGCCGTCAACACGAAAACGGATCACCAGACTCTTTTCAAACGTCTCGATGTGGATATCCGAAGCGCCTTCTTTAATCGCCTCTGCCAGCATGGCGTTGATCAGTTTGATGATCGGCGCATCGTCGTCACTTTCCAGCAGATCTTCCGTTTCCGGCAGTTCTTCTGCGAGGGTAAAAAAGTCCTCGGCAGAACCGAGATCTTCCATCAGTTGGCGAGCCTCGGAAGAGTCCCGCTGCCAGACCGCATTCAGCCGCTGTTCAAATTCGGCTTCGTCGATTTGCCGCAGCGTAAAGGGCGCGTTCAGCCCCCGCTGCAGCTCCTGCAAAACAGAGAGCGACAACGGATGAACATGGAGGATCTCCAGCGACGCTTCGCACCATGCCACCAGGCTAAACCGACGGCTGAAACTGTAGGGCAGACGCACGGTGTTAGCAGTGGTTTCCTGTGCTACAGGCACCATTAACGCGTTCTCCCGGCATTGAGGAATGCGCGAACTTCCGGCGGTAAGGCCTGGTTTTGCGCTGGCAGTACC  >  NC_012971/2969761‑2970353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑