Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_012971	2,969,387	Δ670 bp	coding (226‑895/1494 nt)	gspE ←	type II secretion system ATPase GspE

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_012971	2969387	2970056	670	59 [0]	[0] 59	gspE	type II secretion system ATPase GspE

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_012971	= 2969386	0 (0.000)	59 (0.620)	39/586	1.1	100%	coding (896/1494 nt)	gspE	type II secretion system ATPase GspE
	?	NC_012971	2970057 =	0 (0.000)					coding (225/1494 nt)	gspE	type II secretion system ATPase GspE

GGCCTGGCGGCAGTCGGGGCACAGCGTACGGACCAGTCGCTGCGCCATCACGCCCGTCAGACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGTCTGACCGATCCCTTCAATCATGTATTCGATGGGATCTTCAACCGTGAGAATGTTGCGCGAATGGTTGTTCAGCTCCTGCAATCCGGCGTACAGCGTGGTGCTTTTGCCGGAACCTGTCGGCCCCGTCACCAGAAAGATGCCGTGCGGTTTGTGTAACAGCTGGCGCAACTGCGCAGTCAGTTCGTGACTTAAACCCAGACGCTCCAGCGTCAGGCGGGCCTGGTTTTTGTCCAGCAGTCGCAGCACCACGCGCTCGCCCCAGGCGGAAGGCATGGTGGAGACGCGCACGTCGATCG  >  NC_012971/2969091‑2969683                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                          ggCCTGGCGGCAGTCGGGGCACAGCGTACGGACCAGTCGCTGCGCCATCACGCCCGTCAGACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGcca                                                                                                                                                                                                                                                                                                        >  2:690553‑M1/1‑296 (MQ=255)     tGGCGGCAGTCGGGGNACAGCGTACGGACCAGTCNCTGCGCCATCACGCCCGTCAGACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCATTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTNTTAACCTGTGccagac                                                                                                                                                                                                                                                                                                     <  1:682975‑M1/298‑7 (MQ=255)     tGGCGGCAGTCGGGGNACAGCGTACGGACCAGTCNCTGCGCCATCACGCCCGTCAGACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTNTTAACCTGTGccagac                                                                                                                                                                                                                                                                                                     <  1:682666‑M1/298‑7 (MQ=255)     tGGCGGCAGTCGGGGCACAGCGTACGGACCAGTCGCTGCGCCATCACGCCCGTCAGACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagac                                                                                                                                                                                                                                                                                                     <  1:781757‑M1/298‑7 (MQ=255)          gcAGTCGGGGCACAGCGTACGGACCAGTCGCTGCGCCATCACGCCCGTCAGACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattc                                                                                                                                                                                                                                                                                              <  1:455352‑M1/300‑14 (MQ=255)                ggggCACAGCGTACGGACCAGTCGCTGCGCCATCACGCCCGTCAGACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgc                                                                                                                                                                                                                                                                                        <  1:757851‑M1/300‑20 (MQ=255)                        gCGTACGGACCAGTCGCTGCGCCATCACGCCCGTCAGACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaca                                                                                                                                                                                                                                                                                >  1:472738‑M1/1‑273 (MQ=255)                        gCGTACGGACCAGTCGCTGCGCCATCACGCCCGTCAGACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaa                                                                                                                                                                                                                                                                                >  2:691815‑M1/1‑273 (MQ=255)                           tACGGACCAGTCGCTGCGCCATCACGCCCGTCAGACTGGAAGAGAGCAGTAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGGATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaatt                                                                                                                                                                                                                                                                              <  2:196896‑M1/299‑30 (MQ=255)                                     tCGCTGCGCCATCACGCCCGTCAGACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAANCGCGCGCAGGCAACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtc                                                                                                                                                                                                                                                                    >  2:630598‑M1/1‑260 (MQ=255)                                                         tCAGACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaaggg                                                                                                                                                                                                                                               <  1:761038‑M1/300‑61 (MQ=255)                                                            gACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCAATACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgc                                                                                                                                                                                                                                            >  2:754834‑M1/1‑237 (MQ=255)                                                                                ggCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCAAGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagct                                                                                                                                                                                                                        >  1:36959‑M1/1‑217 (MQ=255)                                                                                       cccccATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaa                                                                                                                                                                                                                 >  1:186335‑M1/1‑210 (MQ=255)                                                                                         cccATATCCTGCAAACNCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcggccgctgttcaaattcggcttcgtcgatttgccgcagcgtanagggcgcgttcagcccccnctgcagctcctgcaaaa                                                                                                                                                                                                               >  1:687394‑M1/1‑208 (MQ=255)                                                                                            atatCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaac                                                                                                                                                                                                              <  1:462265‑M1/298‑94 (MQ=255)                                                                                                               tCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaac                                                                                                                                                                                         <  1:188012‑M1/300‑115 (MQ=255)                                                                                                                       aCCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggag                                                                                                                                                                                   <  1:642485‑M1/298‑121 (MQ=255)                                                                                                                             gtgtTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcngcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctcca                                                                                                                                                                           >  1:151998‑M1/1‑172 (MQ=255)                                                                                                                             gtgtTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctcca                                                                                                                                                                           >  1:293962‑M1/1‑172 (MQ=255)                                                                                                                                ttGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgntttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagc                                                                                                                                                                         >  2:623889‑M1/1‑169 (MQ=255)                                                                                                                                          gCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgca                                                                                                                                                              >  1:137009‑M1/1‑159 (MQ=255)                                                                                                                                          gCGTGGAAAGGACCAAGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGAGGATTTCACCGACCATCAATACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCAAGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaatccggcttcgtcgctttgccgcagcgtaaagggcgcgttccgcccacgctgcagctcctgcaaaacagagagcgaaaacagatgaacacggaggagctccagtgccgctccgca                                                                                                                                                              >  2:384005‑M1/1‑159 (MQ=255)                                                                                                                                            gTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcac                                                                                                                                                             <  2:293962‑M1/299‑143 (MQ=255)                                                                                                                                            gTGGAAAGGACCAGGTGTCAGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcac                                                                                                                                                             <  1:612160‑M1/299‑143 (MQ=255)                                                                                                                                             tGGAAAGGACCAGGTGTCCGGTAATTGAAGCCTGTACGGCGGTTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcac                                                                                                                                                             <  2:151998‑M1/298‑143 (MQ=255)                                                                                                                                              ggAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccat                                                                                                                                                          <  1:289597‑M1/300‑146 (MQ=255)                                                                                                                                                   ggACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccac                                                                                                                                                     <  1:775937‑M1/300‑151 (MQ=255)                                                                                                                                                                ggTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaacc                                                                                                                                          <  1:101872‑M1/298‑162 (MQ=255)                                                                                                                                                                ggTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCNGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaacc                                                                                                                                          <  1:157925‑M1/298‑162 (MQ=255)                                                                                                                                                                             tGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTACTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagcagctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgt                                                                                                                           <  1:114704‑M1/300‑177 (MQ=255)                                                                                                                                                                                  ggCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggc                                                                                                                      <  2:556639‑M1/300‑182 (MQ=255)                                                                                                                                                                                                    ggTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagc                                                                                                     <  1:522954‑M1/299‑199 (MQ=255)                                                                                                                                                                                                     gTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcag                                                                                                   <  2:806190‑M1/300‑201 (MQ=255)                                                                                                                                                                                                     gTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcag                                                                                                   <  1:718467‑M1/300‑201 (MQ=255)                                                                                                                                                                                                     gTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgtgagcag                                                                                                   <  1:718457‑M1/300‑201 (MQ=255)                                                                                                                                                                                                               tttCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctg                                                                                         <  1:454436‑M1/300‑211 (MQ=255)                                                                                                                                                                                                                 tCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttc                                                                                            >  1:723762‑M1/1‑88 (MQ=255)                                                                                                                                                                                                                 tCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgaacggtgttagaagtggtttc                                                                                            >  2:237296‑M1/1‑88 (MQ=255)                                                                                                                                                                                                                       aCCATCACTACATCCGGTTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCTACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacag                                                                                 <  1:272241‑M1/300‑219 (MQ=255)                                                                                                                                                                                                                       aCCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacag                                                                                 <  1:272001‑M1/300‑219 (MQ=255)                                                                                                                                                                                                                       aCCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacag                                                                                 <  2:675177‑M1/300‑219 (MQ=255)                                                                                                                                                                                                                        ccATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctaca                                                                                  >  1:540603‑M1/1‑81 (MQ=255)                                                                                                                                                                                                                          aTCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggca                                                                              <  2:691313‑M1/300‑222 (MQ=255)                                                                                                                                                                                                                          aTCACTACATCCGGGGCCTGACGCAAAATCGCGCGCAGGCCACGGGCAAAGGTCATGCCGACACGGGTGTTAACCGGTGccagaccgcattcagccgctgttcaaatttggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccatcgacgcttcgcaccatgccaccatgctaaacctacggctgaaactgtaggacaaacgcacggtgtcagcactggttccctatcctcccc                                                                                 <  2:280514‑M1/297‑219 (MQ=255)                                                                                                                                                                                                                                                      tCGCGCGCAGGCCACGGGCGAAGGTCATGCCTACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggcaccattaacgcgttctcccggcattgagg                                                  <  2:313199‑M1/300‑250 (MQ=255)                                                                                                                                                                                                                                                      tCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattctgcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcaactcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgactgctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggcaccattaacgcgttctcccggcattgagg                                                  <  2:313476‑M1/300‑250 (MQ=255)                                                                                                                                                                                                                                                        gcgcgcAGGCCACGGTCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggcaccattaacgcgttctcccggcatttaggaa                                                <  2:387020‑M1/300‑252 (MQ=255)                                                                                                                                                                                                                                                               ggCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagcctctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggcaccattaacgcgttctcccggcattgaggaatgcgcga                                         >  1:402439‑M1/1‑42 (MQ=255)                                                                                                                                                                                                                                                               ggCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggcaccattaacgcgttctcccggcattgaggaatgcgcga                                         >  2:542509‑M1/1‑42 (MQ=255)                                                                                                                                                                                                                                                               ggCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggcaccattaacgcgttctcccggcattgaggaatgcgcga                                         >  1:382282‑M1/1‑42 (MQ=255)                                                                                                                                                                                                                                                                 ccACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggcaccattaacgcgttctcccggcattgaggaatgcgcgaa                                        >  2:545807‑M1/1‑40 (MQ=255)                                                                                                                                                                                                                                                                                 tGCCGACGCGGGTGTTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggcaccattaacgcgttctcccggcattgaggaatgcgcgaacttccggcggtaaggcc                       <  1:530596‑M1/300‑277 (MQ=255)                                                                                                                                                                                                                                                                                            gtgtTAACCTGTGccanaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggcaccattaacgcgttctcccggcattgaggantgcgcgaacttccggcggnaaggcctggttttgcgc            >  1:673343‑M1/1‑13 (MQ=255)                                                                                                                                                                                                                                                                                            gtgtTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggccccattaacgcgttctcccggcattgaggaatgcgcgaacttccggcggtaaggcctggttttgcgc            >  2:441171‑M1/1‑13 (MQ=255)                                                                                                                                                                                                                                                                                            gtgtTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggcaccattaacgcgttctcccggcattgaggaatgcgcgaacttccggcggtaaggcctggttttgcgc            >  1:13248‑M1/1‑13 (MQ=255)                                                                                                                                                                                                                                                                                              gtTAACCTGTGccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaacaggctaaaccgacggctgaaactgtagggcagaagcacggtgttagcagtggtttcctgtgctacaggcaccattaacgcgttctcccggcattgaggaatgcgcgaacttccggcggtaaggactggttttgcgct           >  2:169458‑M1/1‑11 (MQ=255)                                                                                                                                                                                                                                                                                                      gtgccagaccgcattcagccgctgttcaaattcggcttcgtcgatttgccgcagcgtaaagggcgcgttcagcccccgctgcagctcctgcaaaacagagagcgacaacggatgaacatggaggatctccagcgacgcttcgcaccatgccaccaggctaaaccgacggctgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggcaccattaacgcgttctcccggcattgaggaatgcgcgaacttccggcggtaaggcctggttttgcgctggcagtacc  >  1:741173‑M1/1‑3 (MQ=255)                                                                                                                                                                                                                                                                                                      gtgccaaaccgcatccagccgctgttcaaattcggctccgtcgatttgccgcagcgtaaaggacacgctcagcacccgctgcagctcctgcaagacaaagagcgacaacggatgaacatgaagaatctacagcgacgctcagcaccataccaccaggctaaaccgacgactgaaactgtagggcagacgcacggtgttagcagtggtttcctgtgctacaggtaccattaccgcgttctcccggcattgaggaatacgcgaacttcc                                   >  2:335069‑M1/1‑3 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                          GGCCTGGCGGCAGTCGGGGCACAGCGTACGGACCAGTCGCTGCGCCATCACGCCCGTCAGACTGGAAGAGAGCAGGAAAGGCTCTACCCCCATATCCTGCAAACGCGTAATCGCCCCCACCGCCGTGTTGGTATGCAGCGTGGAAAGGACCAGGTGTCCGGTAAGTGAAGCCTGGACGGCGATTTCTGCGGTTTCGGTATCGCGGATTTCACCGACCATCACTACATCCGGGTCCTGACGCAAAATCGCGCGCAGGCCACGGGCGAAGGTCATGCCGACGCGGGTGTTAACCTGTGTCTGACCGATCCCTTCAATCATGTATTCGATGGGATCTTCAACCGTGAGAATGTTGCGCGAATGGTTGTTCAGCTCCTGCAATCCGGCGTACAGCGTGGTGCTTTTGCCGGAACCTGTCGGCCCCGTCACCAGAAAGATGCCGTGCGGTTTGTGTAACAGCTGGCGCAACTGCGCAGTCAGTTCGTGACTTAAACCCAGACGCTCCAGCGTCAGGCGGGCCTGGTTTTTGTCCAGCAGTCGCAGCACCACGCGCTCGCCCCAGGCGGAAGGCATGGTGGAGACGCGCACGTCGATCG  >  NC_012971/2969091‑2969683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑