breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 67 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913190,8430CA42.3% 146.8 / 16.8 130intergenic (+244/‑14)rpsB/tsf30S ribosomal subunit protein S2/protein chain elongation factor EF‑Ts
*NC_0009132,493,4320GC42.1% 16.8 / 50.7 38intergenic (‑178/+335)frc/yfdXformyl‑CoA transferase/protein YfdX
*NC_0009132,477,2650AC39.4% 182.2 / 48.5 127L122* (TTA→TGA) ‡dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009132,472,8170CA36.5% 153.0 / 11.8 96G22V (GGA→GTA) yfdNuncharacterized protein YfdN
*NC_0009131,427,9920GT35.2% 213.4 / 21.6 159P245P (CCC→CCAinsH5IS5 family transposase and trans‑activator
*NC_0009131,375,7230CA34.9% 167.5 / 14.4 106S254Y (TCC→TAC) ycjQD‑guloside 3‑dehydrogenase
*NC_00091358,0650CA34.6% 223.8 / 24.9 129P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_000913320,8450CA34.0% 126.4 / 16.4 97R207R (CGG→AGG) rclRDNA‑binding transcriptional activator RclR
*NC_000913442,4710CA33.8% 210.1 / 13.8 139S308Y (TCC→TAC) thiItRNA uridine 4‑sulfurtransferase
*NC_0009132,288,4180GT33.7% 277.8 / 28.3 166pseudogene (497/2525 nt)yejOadhesin‑like autotransporter YejO
*NC_0009133,741,8710GT33.1% 263.8 / 29.7 157S221Y (TCC→TAC) plaRDNA‑binding transcriptional repressor PlaR
*NC_0009131,922,8150CA31.6% 241.5 / 19.2 139G52V (GGA→GTA) pphAphosphoprotein phosphatase 1
*NC_0009132,767,4450CA31.4% 240.7 / 27.3 121intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009132,990,4800CA30.5% 324.4 / 11.3 190intergenic (‑120/‑788)yqeK/ygeGprotein YqeK/TPR repeat‑containing putative chaperone YgeG
*NC_0009131,351,4630CA30.2% 217.2 / 18.7 139E100* (GAG→TAG) ycjDDUF559 domain‑containing protein YcjD
*NC_0009132,477,2640TC30.2% 253.4 / 10.7 127L122L (TTA→TTG) ‡dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009132,722,8380GT29.7% 276.3 / 12.3 145E38* (GAG→TAG) pssAphosphatidylserine synthase
*NC_0009133,115,1050GT29.6% 206.8 / 12.6 108L1336L (CTC→CTAyghJputative lipoprotein YghJ
*NC_0009131,756,3530CA29.3% 259.6 / 14.1 150S219Y (TCC→TAC) pykFpyruvate kinase 1
*NC_0009133,911,5330CA29.1% 301.8 / 21.5 175intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase

Marginal new junction evidence (lowest skew 10 of 35 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 780629125 (1.010)27 (0.290) 21/436 3.1 23.1% noncoding (76/76 nt) lysT tRNA‑Lys
?NC_000913 780919 = 87 (0.950)intergenic (+1/‑149) lysW/valZ tRNA‑Lys/tRNA‑Val
* ? NC_000913 = 128732973 (0.590)11 (0.130) 11/414 5.3 17.3% intergenic (‑1/+209) tyrV/tyrT tRNA‑Tyr/tRNA‑Tyr
?NC_000913 1287624 = 54 (0.620)intergenic (‑2/+158) tyrT/purU tRNA‑Tyr/formyltetrahydrofolate deformylase
* ? NC_000913 = 3648146140 (1.130)14 (0.140) 12/472 5.7 10.8% intergenic (+2/‑148) agrA/agrB small RNA AgrA/small regulatory RNA AgrB
?NC_000913 3648378 = 118 (1.190)intergenic (+3/‑150) agrB/arsR small regulatory RNA AgrB/DNA‑binding transcriptional repressor ArsR
* ? NC_000913 = 4542690189 (1.530)16 (0.130) 14/570 6.2 9.0% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 142 (1.180)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 460616697 (0.780)9 (0.100) 8/410 6.4 11.1% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 77 (0.890)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 4542682 =176 (1.420)13 (0.110) 13/570 6.5 7.7% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 143 (1.190)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 2653419111 (0.900)13 (0.110) 11/538 6.9 10.6% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 117 (1.030)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 = 294746793 (0.750)8 (0.090) 7/422 7.0 10.5% intergenic (+4/‑30) metZ/metW tRNA‑Met/tRNA‑Met
?NC_000913 2947688 = 69 (0.780)intergenic (+5/+69) metV/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
* ? NC_000913 = 251811695 (0.770)7 (0.080) 7/426 7.0 9.9% noncoding (1/76 nt) alaX tRNA‑Ala
?NC_000913 2518232 = 58 (0.650)intergenic (‑1/‑235) alaW/yfeC tRNA‑Ala/DNA‑binding transcriptional dual regulator YfeC
* ? NC_000913 = 78101688 (0.710)13 (0.120) 9/514 7.3 13.2% intergenic (+98/‑52) lysW/valZ tRNA‑Lys/tRNA‑Val
?NC_000913 781809 = 94 (0.870)intergenic (+157/‑276) lysQ/nadA tRNA‑Lys/quinolinate synthase