breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 43 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,911,5330CA41.2% 147.3 / 31.2 131intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009134,427,8220CA35.9% 170.2 / 12.0 106intergenic (‑400/+257)yjfZ/ytfBDUF2686 domain‑containing protein YjfZ/cell division protein YtfB
*NC_0009132,767,4450CA32.9% 160.2 / 19.6 83intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009133,401,3450GT32.1% 189.0 / 13.3 109intergenic (‑258/+47)mreB/csrDdynamic cytoskeletal protein MreB/regulator of CsrB and CsrC decay
*NC_0009132,722,8380GT31.9% 212.9 / 15.5 116E38* (GAG→TAG) pssAphosphatidylserine synthase
*NC_0009132,990,4800CA31.7% 290.9 / 29.0 167intergenic (‑120/‑788)yqeK/ygeGprotein YqeK/TPR repeat‑containing putative chaperone YgeG
*NC_0009132,288,4180GT31.3% 220.1 / 24.7 134pseudogene (497/2525 nt)yejOadhesin‑like autotransporter YejO
*NC_000913847,9550GA31.3% 148.5 / 46.7 115A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_0009132,427,7160CA30.9% 208.7 / 16.3 111E26* (GAG→TAG) argTlysine/arginine/ornithine ABC transporter periplasmic binding protein
*NC_0009132,073,6130GT30.2% 160.3 / 16.3 86R692L (CGG→CTG) fluself recognizing antigen 43 (Ag43) autotransporter
*NC_00091358,0650CA30.0% 159.5 / 15.3 100P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009131,922,8150CA30.0% 144.8 / 21.3 100G52V (GGA→GTA) pphAphosphoprotein phosphatase 1
*NC_000913141,8490CA29.5% 254.1 / 17.0 140P140Q (CCG→CAG) hpthypoxanthine phosphoribosyltransferase
*NC_0009134,360,9650CA29.5% 353.2 / 20.5 173E324* (GAG→TAG) cadCDNA‑binding transcriptional activator CadC
*NC_0009134,296,2680TC29.0% 39.1 / 12.9 31intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009133,054,8860GT28.9% 224.4 / 30.6 129Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_0009131,779,5830GT28.7% 207.5 / 13.9 115intergenic (‑282/‑34)ydiP/ydiQputative DNA‑binding transcriptional regulator YdiP/putative electron transfer flavoprotein subunit YdiQ
*NC_0009133,054,8940GT28.6% 192.3 / 23.2 120P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_0009132,876,7150GT28.3% 236.0 / 16.2 127P45P (CCG→CCTiapalkaline phosphatase isozyme conversion protein
*NC_0009131,502,7720GA28.3% 189.2 / 37.0 120A106T (GCG→ACG) ydcLDUF3313 domain‑containing lipoprotein YdcL

Marginal new junction evidence (lowest skew 10 of 29 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4057551102 (1.020)26 (0.260) 21/590 3.5 20.3% coding (484/1410 nt) glnA glutamine synthetase
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 4057547 =95 (0.950)25 (0.250) 18/590 4.1 20.8% coding (488/1410 nt) glnA glutamine synthetase
?NC_000913 4498181 = NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 4542682 =133 (1.330)17 (0.180) 16/572 4.4 12.2% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 116 (1.200)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 3186096 =NA (NA)18 (0.180) 16/588 4.5 13.2% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4353014 118 (1.190)coding (107/174 nt) ghoT toxin of the GhoTS toxin‑antitoxin system
* ? NC_000913 = 3187426NA (NA)15 (0.150) 13/588 5.3 11.1% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 4353010 = 120 (1.210)coding (103/174 nt) ghoT toxin of the GhoTS toxin‑antitoxin system
* ? NC_000913 = 69674168 (0.680)11 (0.150) 8/434 5.4 15.3% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 72 (0.980)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 460616690 (0.900)8 (0.110) 7/412 5.5 12.5% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 49 (0.700)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 78128273 (0.730)12 (0.120) 12/574 5.5 14.5% intergenic (+60/‑87) lysY/lysZ tRNA‑Lys/tRNA‑Lys
?NC_000913 781771 = 70 (0.720)intergenic (+119/‑314) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 294746772 (0.720)8 (0.110) 7/424 5.6 15.4% intergenic (+4/‑30) metZ/metW tRNA‑Met/tRNA‑Met
?NC_000913 2947688 = 36 (0.500)intergenic (+5/+69) metV/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
* ? NC_000913 = 275399184 (0.840)13 (0.130) 11/590 5.9 13.4% intergenic (+45/+17) bamE/yfjF outer membrane protein assembly factor BamE/putative component of the Rsx system
?NC_000913 = 4503515 NA (NA)noncoding (1/1426 nt) IS4 repeat region